草地学报

草地学报 ›› 2019, Vol. 27 ›› Issue (6): 1786-1792.DOI: 10.11733/j.issn.1007-0435.2019.06.040

• 研究专报 • 上一篇    

脱落酸对混合苏打盐碱胁迫苜蓿幼苗营养器官矿质元素含量的影响

李波1, 邬婷婷1, 方志坚1, 李红2, 杨曌2, 林浩1   

  1. 1. 齐齐哈尔大学生命科学与农林学院, 抗性基因工程与寒地生物多样性保护黑龙江省重点实验室, 黑龙江 齐齐哈尔 161006;
    2. 黑龙江省农业科学院畜牧兽医分院, 黑龙江 齐齐哈尔 161005
  • 收稿日期:2019-08-16 修回日期:2019-09-17 出版日期:2019-12-15 发布日期:2019-12-31
  • 作者简介:李波(1962-),女,辽宁鞍山人,教授,主要从事细胞生物学研究,E-mail:libo1962@163.com
  • 基金资助:
    黑龙江省应用技术研究与开发计划重大项目（GA15B105-5）；黑龙江省省属高等学校基本科研业务费科研项目（135209267，YSTSXK201886）；齐齐哈尔市科技计划一般项目（NYGG-201916）共同资助

Effects of ABA on Mineral Elements in Vegetative Organs of Alfalfa Seedlings under Mixed Soda Salt-alkaline Stress

LI Bo1, WU Ting-ting1, FANG Zhi-jian1, LI Hong2, YANG Zhao2, LIN Hao1   

  1. 1. College of Agriculture, Forestry and Life Sciences, Qiqihar University, Heilongjiang Provincial Key Laboratory of Resistance Gene Engineering and Protection of Biodiversity in Cold Areas, Qiqihar, Heilongjiang Province 161006, China;
    2. Institute of Animal Husbandry and Veterinary of Heilongjiang Academy of Agricultural Sciences, Qiqihar, Heilongjiang Province 161005, China
  • Received:2019-08-16 Revised:2019-09-17 Online:2019-12-15 Published:2019-12-31

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摘要： 为了探究外源脱落酸（Abscisic acid，ABA）对混合苏打盐碱胁迫下紫花苜蓿（Medicago sativa L.）幼苗营养器官离子含量的影响，本试验以WL343HQ苜蓿种子为试验材料，对苜蓿幼苗进行150 mmol·L-1混合苏打盐碱胁迫和25，50，75和100 μmol·L-1ABA缓解处理，采用原子吸收法测定ABA缓解混合苏打盐碱胁迫下苜蓿幼苗根、茎和叶中钠（Na+）、钾（K+）、钙（Ca2+）和镁（Mg2+）离子含量的变化。结果表明：混合苏打盐碱胁迫导致苜蓿幼苗各器官的Na+，K+，Ca2+，Mg2+含量产生不同的变化；其中根中Na+和Ca2+含量增加，K+和Mg2+含量降低，茎中Na+，K+，Mg2+含量增加，Ca2+含量降低；叶中Na+和Mg2+含量增加，K+和Ca2+含量降低。经不同浓度的ABA处理的混合苏打盐碱胁迫后苜蓿幼苗各器官中，根中Na+含量、根和叶K+含量、根和叶Ca2+含量、根、茎和叶Mg2+含量均随ABA浓度增加而增加，茎和叶Na+含量随着ABA浓度的升高而下降，茎中K+和Ca2+含量随着ABA浓度的升高无明显变化。各器官中的Na+/K+，Na+/Ca2+和Na+/Mg2+比值均随着ABA浓度的升高而下降。可见，混合苏打盐碱胁迫影响苜蓿幼苗对Na+，K+，Ca2+，Mg2+在根、茎、叶营养器官的分布，与苜蓿幼苗的抗盐碱有一定的相关性。

关键词: 苜蓿, 脱落酸, 混合苏打盐碱胁迫, 营养器官, 矿质元素

Abstract: In order to investigate the effect of exogenous abscisic acid on the ionic content of vegetative organs of alfalfa (Medicago sativa L.) seedlings with mixed soda salt-alkaline,WL343HQ alfalfa seed was used as the test material,and the seedlings were treated with 150 mmol·L-1 mixed soda salt-alkaline stress and 25,50,75 and 100 μmol·L-1 ABA. The changes of sodium (Na+),potassium (K+),calcium (Ca2+) and magnesium (Mg2+) ions in roots,stems and leaves of alfalfa seedlings were studied by atomic absorption spectrometry. The results showed that the contents of Na+,K+,Ca2+ and Mg2+ in different organs of alfalfa seedlings were different under mixed soda saline-alkali stress. The content of Na+ and Ca2+ in roots increased,the contents of K+ and Mg2+ decreased,and the contents of Na+,K+ and Mg2+ in stems increased,Ca2+ content decreased;Na+ and Mg2+ levels in leaves increased,and K+ and Ca2+ levels decreased. Root Na+ content,root and leaf K+ content,root and leaf Ca2+ content,root,stem and leaf Mg2+ content increased with the increase of ABA concentration in different organs of alfalfa seedlings treated with different concentrations of ABA. The content of Na+ in stems and leaves decreased with the increase of ABA concentration. The contents of K+ and Ca2+ in stems didn't change significantly with the increase of ABA concentration. The ratios of Na+/K+,Na+/Ca2+ and Na+/Mg2+ in each organ decreased with the increase of ABA concentration. It can be seen that the mixed soda saline-alkali stress affects the distribution of Na+,K+,Ca2+ and Mg2+ in the vegetative organs of roots,stems and leaves of alfalfa seedlings,and has certain correlation with the salt-tolerance of alfalfa seedlings.

Key words: Alfalfa, Abscisic acid(ABA), Mixed soda salt-alkaline stress, Vegetative organs, Mineral elements

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