富氢水对草地早熟禾耐盐性的影响以及与抗氧化酶活性的关系

doi:10.11733/j.issn.1007-0435.2021.07.008

草地学报 ›› 2021, Vol. 29 ›› Issue (7): 1436-1445.DOI: 10.11733/j.issn.1007-0435.2021.07.008

富氢水对草地早熟禾耐盐性的影响以及与抗氧化酶活性的关系

张韦钰, 王春勇, 杜红梅

上海交通大学设计学院, 上海 200240

收稿日期:2020-11-21 修回日期:2021-03-16 出版日期:2021-07-15 发布日期:2021-07-31
通讯作者: 杜红梅,E-mail:hmdu@sjtu.edu.cn
作者简介:张韦钰(1995-),女,安徽省阜阳人,硕士研究生,主要从事园林植物抗逆生理方面的研究,E-mail:zhangweiyu@sjtu.edu.cn
基金资助:
上海交通大学氢科学中心资助

Effects of Hydrogen-rich Water on Salt-Tolerance of Kentucky Bluegrass and Antioxidant Enzymes Activity

ZHANG Wei-yu, WANG Chun-yong, DU Hong-mei

School of Design, Shanghai Jiaotong University, Shanghai 200240, China

Received:2020-11-21 Revised:2021-03-16 Online:2021-07-15 Published:2021-07-31

摘要/Abstract

摘要： 本研究以草地早熟禾（Poa pratensis）‘Midnight II’为材料，采用50%的富氢水（Hydrogen-rich water，HRW）叶片喷施及灌根的方法，分析HRW处理对200 mM NaCl胁迫条件下草地早熟禾叶片干物质含量、相对含水量、叶绿素含量、电解率外渗（Electrolyte leakage，EL）及抗氧化酶活性的影响。结果表明：HRW处理显著增加了盐胁迫条件下细胞的持水能力，提高了叶片的叶绿素含量，降低了EL值；通过分析0 d，5 d和20 d盐胁迫条件下草地早熟禾叶片活性氧（Reactive oxygen species，ROS）和丙二醛含量，抗氧化酶活性以及抗氧化酶基因表达发现，在盐胁迫，尤其是长时间盐胁迫条件下，HRW处理显著降低了ROS含量，增加细胞膜稳定性，同时提高了长时间盐胁迫条件下部分抗氧化酶的活性，且诱导了抗氧化酶基因CAT2，APX1，MR的下调表达。研究结果表明，在HRW对草地早熟禾耐盐性的影响中，ROS的含量变化可能发挥关键作用。研究结果对草坪草耐盐生理以及氢气生物学的研究具有一定的指导意义。

关键词: 草地早熟禾, 富氢水, 盐胁迫, 活性氧, 抗氧化酶

Abstract: In this study,the influences of hydrogen-rich water (HRW) treatment on the dry matter content,relative water content,chlorophyll contents, electrolyte leakage (EL) and antioxidant enzymes activity of Kentucky bluegrass (Poa pratensis) ‘Midnight II’ were examined by spraying 50% HRW on leaves and roots. The results showed that HRW treatment significantly increased water holding capacity of cells and chlorophyll contents of leaves and decreased EL under salt stress. By analyzing reactive oxygen species (ROS) and malondialdehyde contents,antioxidant enzyme activities and antioxidant enzyme gene expression under 0 d,5 d and 20 d of salt stress in Kentucky bluegrass,it was found that under salt stress,especially under long-time of salt stress,ROS contents were decreased,and cell membrane stability increased. Meanwhile,HRW treatment increased the activities of some antioxidant enzymes under long-time of salt stress. In addition,HRW induced down-regulated expression of antioxidant enzyme genes CAT2,APX1 and MR. Research indicates that the changes of ROS content might play a key role in the effects of HRW on salt-tolerance of Kentucky bluegrass. The results had guiding significance for the research of turfgrass salt-tolerance physiology and hydrogen biology in the future.

Key words: Kentucky bluegrass, Hydrogen-rich water, Salt stress, Reactive oxygen species, Antioxidant enzymes

中图分类号:

S812.4

张韦钰, 王春勇, 杜红梅. 富氢水对草地早熟禾耐盐性的影响以及与抗氧化酶活性的关系[J]. 草地学报, 2021, 29(7): 1436-1445.

ZHANG Wei-yu, WANG Chun-yong, DU Hong-mei. Effects of Hydrogen-rich Water on Salt-Tolerance of Kentucky Bluegrass and Antioxidant Enzymes Activity[J]. Acta Agrestia Sinica, 2021, 29(7): 1436-1445.

0
/ / 推荐

导出引用管理器 EndNote|Ris|BibTeX

链接本文: https://manu40.magtech.com.cn/Jweb_cdxb/CN/10.11733/j.issn.1007-0435.2021.07.008

https://manu40.magtech.com.cn/Jweb_cdxb/CN/Y2021/V29/I7/1436

参考文献

[1] Tang X,Mu X,Shao H,et al. Global plant-responding mechanisms to salt stress:Physiological and molecular levels and implications in biotechnology[J]. Critical Reviews in Biotechnology,2015,35(4):425-437
[2] Hassani A,Azapagic A,Shokri N. Predicting long-term dynamics of soil salinity and sodicity on a global scale[J]. Proceedings of the National Academy of Sciences of the United States of America,2021,117(52):33017-33027
[3] Hanin M,Ebel C,Ngom M,et al. New insights on plant salt tolerance mechanisms and their potential use for breeding[J]. Frontiers in Plant Science,2016,7(29):1787
[4] Mittler R. Oxidative stress,antioxidants and stress tolerance[J]. Trends in Plant Science,2002,7(9):405-410
[5] Apel K,Hirt H. Reactive oxygen species:Metabolism,oxidative stress,and signal transduction[J]. Annual Review of Plant Biology,2004,7(55):373-399
[6] 孙吉雄. 草坪学(第二版)[M]. 北京:中国农业出版社,2004:86-87
[7] 张强,刘宁芳,向佐湘,等. 盐碱胁迫对草地早熟禾生长和生理代谢的影响[J]. 草业学报,2017,26(12):67-76
[8] Koch M J,Huang,B,Bonos S A. Salinity tolerance of Kentucky bluegrass cultivars and selections using an overhead irrigated screening technique[J]. Crop Science,2011,51(6):2846-2857
[9] 沈文飚,苏久厂,孙学军. 氢气植物学效应的研究进展[J]. 南京农业大学学报,2018,41(3):392-401
[10] Xie Y,Mao Y,Lai D,et al. H₂ Enhances Arabidopsis salt tolerance by manipulating ZAT10/12-mediated antioxidant defence and controlling sodium exclusion[J]. PLoS ONE,2012,7(11):e49800
[11] 赵苹艺,胡玉龙,李雪华,等. 不同浓度H₂对NaCl胁迫下甘薯苗生理指标的影响[J]. 湖北农业科学,2016,55(12):3013-3017
[12] Zeng J,Zhang M,Sun X. Molecular hydrogen is involved in phytohormone signaling and stress responses in plants[J]. PLoS ONE,2013,8(8):e71038
[13] Xu S,Zhu S,Jiang Y,et al. Hydrogen-rich water alleviates salt stress in rice during seed germination[J]. Plant and Soil,2013,370(1-2):47-57
[14] Wei X,Chen J,Chen H,et al. Hydrogen-rich water ameliorates the toxicity induced by Ca(NO₃)₂ excess through enhancing antioxidant capacities and re-establishing nitrate homeostasis in Brassica campestris spp. chinensis L. seedlings[J]. Acta Physiologiae Plantarum,2021,43(3):50-62
[15] 田婧芸,杨利艳,王创云,等. 外源氢气对玉米幼苗耐盐性的影响[J]. 湖南师范大学自然科学学报,2018,41(6):23-30
[16] 张海那,范海延,于洋,等. 氢气调节植物生长发育和提高植物抗逆性的研究进展[J]. 西北植物学报,2017,37(2):402-407
[17] 张燕,朱慧森,白永超,等. 3个居群野生草地早熟禾耐盐性比较研究[J]. 草地学报,2018,26(5):1215-1222
[18] 徐冰,韩烈保,姚娜,等. 草地早熟禾转CMO-BADH双基因和转CMO基因耐盐性分析[J]. 草地学报,2008,16(4):353-358
[19] 安勐颍,孙珊珊,濮阳雪华,等. 外源亚精胺调控草地早熟禾幼苗耐盐性的研究[J]. 草业学报,2014,23(6):207-216
[20] Zhang K,Lyu W,Gao Y,et al. Choline-mediated lipid reprogramming as a dominant salt tolerance mechanism in grass species lacking glycine betaine[J]. Plant Cell Physiol,2021,61(12):2018-2030
[21] Arghavani M,Kafi M,Babalar M,et al. Improvement of salt tolerance in Kentucky bluegrass by trinexapac-ethyl[J]. HortScience,2012,47(8):1163-1170
[22] Cui W,Gao C,Fang P,et al. Alleviation of cadmium toxicity in Medicago sativa by hydrogen-rich water[J]. Journal of Hazardous Materials,2013,260(15):715-724
[23] Barrs H,Weatherley P. Re-Examination of the relative turgidity technique for estimating water deficits in leaves[J]. Australian Journal of Biological Sciences,1962,15(3):413-428
[24] Blum A,Ebercon A. Cell membrane stability as a measure of drought and heat tolerance in wheat[J]. Crop Science,1981,21(1):43-47
[25] Arnon D I. Copper enzymes in isolated chloroplasts.Polyphenoloxidase in Beta vulgaris[J]. Plant Physiology,1949,24(1):1-15
[26] Velikova V,Yordanov I,Edreva A. Oxidative stress and some antioxidant systems in acid rain-treated bean plants protective role of exogenous polyamines[J]. Plant Science,2000,151(1):59-66
[27] Elstner E F,Heupel A. Inhibition of nitrite formation from hydroxylammoniumchloride:A simple assay for superoxide dismutase[J]. Analytical Biochemistry,1976,70(2):616-620
[28] Zhang J,Kirkham M B. Antioxidant responses to drought in sunflower and sorghum seedlings[J]. New Phytologist,1996,132(3):361-373
[29] Du H,Zhou P,Huang B. Antioxidant enzymatic activities and gene expression associated with heat tolerance in a cool-season perennial grass species[J]. Environmental and Experimental Botany,2013,87(3):159-166
[30] Duncan R R,Carrow R N. Turfgrass molecular genetic improvement for abiotic/edaphic stress resisstance[J]. Advances in agronomy,1999,67(08):233-305
[31] Munns R,Tester M. Mechanisms of salinity tolerance[J]. Annual Review of Plant Biology,2008,59(7):651-681
[32] 汪霞. 多花黑麦草耐盐性评价及相关抗氧化酶基因表达分析[D]. 雅安:四川农业大学,2015:2-3
[33] 刘燕,杨伟,马晖玲,等. 盐胁迫对6种草地早熟禾幼苗生理特性的影响[J]. 甘肃农业大学学报,2019,54(5):140-150
[34] Puyang X,An M,Han L,et al. Protective effect of spermidine on salt stress induced oxidative damage in two Kentucky bluegrass (Poa pratensis L.) cultivars[J]. Ecotoxicology and Environmental Safety,2015,6(117):96-106
[35] 宋韵琼,李朝阳,谢平林,等. 富氢水施用时期和施用方法对小苍兰开花的影响及其生理机制[J]. 上海交通大学学报(农业科学版),2017,35(3):10-16
[36] 江宜龙. 富氢水缓解冻害和盐胁迫对水稻幼苗氧化伤害及种子萌发的抑制[D]. 南京:南京农业大学,2014:34-35
[37] Ohsawa I M,Ishikawa K,Takahashi M,et al. Hydrogen acts as a therapeutic antioxidant by selectively reducing cytotoxic oxygen radicals[J]. Nature Medicine.2007,13(6):688-694
[38] Ohta S. Molecular hydrogen as a preventive and therapeutic medical gas:Initiation,development and potential of hydrogen medicine[J]. Pharmacology and Therapeutics,2014,144(1):1-11

富氢水对草地早熟禾耐盐性的影响以及与抗氧化酶活性的关系

Effects of Hydrogen-rich Water on Salt-Tolerance of Kentucky Bluegrass and Antioxidant Enzymes Activity

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	左郎, 王树森, 马迎梅, 温苏雅勒图, 白刚, 郁鑫明. 火炬树浸提液对两种草坪草种子萌发的影响[J]. 草地学报, 2021, 29(9): 1927-1933.
[2]	王宝强, 赵颖, 朱晓林, 王旺田, 魏小红. 盐碱胁迫对藜麦幼苗叶片光合特性及抗氧化系统的影响[J]. 草地学报, 2021, 29(8): 1689-1696.
[3]	金一锋, 陈阳, 齐欣, 高岩松, 金忠民, 赵清峰, 王琦. 草地早熟禾NRT1/PTR FAMILY 8.3基因的克隆及表达分析[J]. 草地学报, 2021, 29(7): 1397-1405.
[4]	朱琨, 郑桂华, 刘丽杰, 周璐, 李珊珊, 范震宇, 钟玥, 尼尼. 草地早熟禾叶片表皮解剖结构与抗白粉病性的研究[J]. 草地学报, 2021, 29(7): 1430-1435.
[5]	朱琨, 刘丽杰, 周璐, 李珊珊, 范震宇, 吴纪一, 尼尼. 外源激素对染白粉菌草地早熟禾叶片膜透性及表面结构的影响[J]. 草地学报, 2021, 29(6): 1210-1216.
[6]	朱瑞婷, 牛奎举, 张娣君, 杨阳, 宋云, 马晖玲. 外源2,4-表油菜素内酯对镉胁迫下草地早熟禾叶绿素代谢的影响[J]. 草地学报, 2021, 29(4): 635-643.
[7]	夏方山, 王聪聪, 李红玉, 刘曼, 郑川, 张耀丹, 范华芳. 硒引发对紫花苜蓿种子抗氧化性能的影响[J]. 草地学报, 2021, 29(3): 472-477.
[8]	刘备, 宋玉梅, 孙铭, 毛培胜. 燕麦劣变种子吸胀过程中线粒体AsA-GSH循环的生理响应[J]. 草地学报, 2021, 29(2): 211-219.
[9]	张玉霞, 丛百明, 王显国, 张庆昕, 杜晓艳, 田永雷. 苜蓿抗寒性与根系抗氧化酶活性相关性分析[J]. 草地学报, 2021, 29(2): 244-249.
[10]	潘平新, 倪强, 马瑞, 杨永义, 马彦军. 不同盐分处理对黑果枸杞种子萌发和幼苗生长的影响[J]. 草地学报, 2021, 29(2): 342-348.
[11]	张银翠, 姚拓, 赵桂琴, 徐茜, 王振龙, 包康, 撖冬荣. 耐盐促生菌筛选鉴定及对盐胁迫燕麦生长的影响[J]. 草地学报, 2021, 29(12): 2645-2652.
[12]	万婷, 段钧译, 李蒙, 陈智勇. 南荻MlNAC2基因差异表达与耐盐生理响应的相关性分析[J]. 草地学报, 2021, 29(12): 2685-2693.
[13]	叶晓倩, 刘瑞曦, 冯金慧, 邓仕明, 李吉涛, 邓志军. 车前种子黏液对其劣变和萌发抗逆性的影响[J]. 草地学报, 2021, 29(12): 2728-2732.
[14]	王菲, 谭怡, 吕亚茹, 苏文欣, 姜宛彤, 刘宇乐, 严俊鑫. 水杨酸和赤霉素预处理对NaCl胁迫下神香草种子萌发的影响[J]. 草地学报, 2021, 29(12): 2862-2870.
[15]	余淑艳, 周燕飞, 黄薇, 王星, 高雪芹, 伏兵哲. 干旱、盐及旱盐复合胁迫对沙芦草光合和生理特性的影响[J]. 草地学报, 2021, 29(11): 2399-2406.