关键词: 抗坏血酸, 种子引发, 盐胁迫, 燕麦, 抗氧化性能

Abstract: In order to investigate the effect of ascorbic acid （AsA） priming on the mitochondrial antioxidant capacity of oat seedlings under salt stress, oat （Avena sativa） seeds were used as materials for this experiment, which was primed with different concentrations of AsA （0, 1, 2, 4 mmol·L^-1） for 24 h, and the germination test was carried out under different concentrations （0, 75, 150 mmol·L^-1） of NaCl stress, then the changes in antioxidant enzyme activities and the level of lipid peroxidation were measured in the oat seedlings germinated for 10th days. The results showed that priming with AsA increased the activities of antioxidant enzymes in mitochondria of oat seedlings under salt stress comparing with CK, and this effect was closely related to the concentration of AsA and salt. In this experiment, priming with 1 mmol·L^-1 AsA was the optimal treatment for oat seedling to improve the antioxidant performance in mitochondria under 75 mmol·L^-1 NaCl stress, and the activities of superoxide dismutase （SOD）, catalase （CAT）, ascorbate peroxidase （APX）, monodehydroascorbate reductase （MDHAR）, dehydroascorbate reductase （DHAR） and glutathione reductase （GR） reached the maximum, which were 7.50 U·mg^-1,0.30 U·min^-1·g^-1,1.09 U·min^-1·g^-1,0.20 U·min^-1·mg^-1,0.12 U ·min^-1·mg^-1,1.13 U·min^-1·g^-1, respectively, but the contents of H₂O₂ and malondialdehyde （MDA） were also the lowest level of 253.99 mmol·g^-1 and 0.02 μmol·mg^-1, respectively.

Key words: Ascorbic acid, Seed priming, Salt stress, Oat, Oxidation resistance