日本结缕草ZjTCP20基因生物信息学及表达模式分析

doi:10.11733/j.issn.1007-0435.2023.02.003

草地学报 ›› 2023, Vol. 31 ›› Issue (2): 321-329.DOI: 10.11733/j.issn.1007-0435.2023.02.003

• 研究论文 • 上一篇

日本结缕草ZjTCP20基因生物信息学及表达模式分析

杨卓雄, 董笛, 韩烈保¹, 晁跃辉

北京林业大学, 北京 100083

收稿日期:2022-07-01 修回日期:2022-09-28 发布日期:2023-02-28
通讯作者: 韩烈保,E-mail:hanliebao@163.com;晁跃辉,E-mail:chaoyuehui@163.com
作者简介:杨卓雄(1997-),女,汉族,宁夏石嘴山人,硕士研究生,主要从事草坪草分子生物技术研究,E-mail:1794123154@qq.com
基金资助:
国家自然科学基金项目(31971770)资助

Bioinformatics and Expression Pattern Analysis of ZjTCP20 Gene in Zoysia japonica

YANG Zhuo-xiong, DONG Di, HAN Lie-bao¹, CHAO Yue-hui

Beijing Forestry University, Beijing 100083, China

Received:2022-07-01 Revised:2022-09-28 Published:2023-02-28

摘要/Abstract

摘要： TCP是高等植物所特有的一类转录因子，参与调控植物的生长发育、衰老及逆境应答等多个生物学过程。本研究从日本结缕草(Zoysia japonica)中克隆出ZjTCP20基因，并对该基因进行生物信息学分析、自激活检测、表达模式分析等来探究该基因特性。ZjTCP20基因完整编码区为624 bp，编码208个氨基酸。ZjTCP20蛋白无信号肽，具有疏水性，系统发育进化树结果显示其与玉米(Zea mays) ZmTCP20亲缘关系最近。亚细胞定位显示该蛋白定位于叶绿体、细胞质和细胞核上。ZjTCP20基因表达模式结果显示，该基因在幼嫩叶片中大量表达，随植物发育表达量逐渐减少，但茉莉酸甲酯(MeJA)等3个激素对基因的表达量没有显著影响。酵母自激活试验显示ZjTCP20具备自激活活性。本研究为进一步研究ZjTCP20基因提供了科学依据。

关键词: 日本结缕草, TCP20基因, 生物信息学, 表达模式分析

Abstract: TCP is a unique transcription factor in higher plants,paticipating in the regulation of biological processes such as,plant growth and development,aging,stress response,et al.. In this study we cloned ZjTCP20 gene from Zoysia japonica,investigated its characters through bioinformatics analysis,self-activation detection and expression pattern analysis. The complete coding region of ZjTCP20 gene was 624 bp,encoded 208 amino acids. ZjTCP20 protein was hydrophobic without signal peptide,and the phylogenetic tree analysis showed it closely similarity to ZmTCP20 protein of Zea mays. Subcellular localization revealed that the protein was resided in the chloroplast,cytoplasm and nucleus. Expression pattern of ZjTCP20 gene showed that it was highly expressed in young leaves and decreased expression with development,but three hormones including abscisic acid (ABA),methyl jasmonate (MeJA) and salicylic acid (SA) didn't have significant effects on its expression. Yeast self-activation experiment revealed that ZjTCP20 had an autoactivation activity. The study provides a scientific basis for further studies of the ZjTCP20 gene in Zoysia japonica.

Key words: Zoysia japonica, TCP20 gene, Bioinformatics, Analysis of expression patterns

中图分类号:

S688.4

杨卓雄, 董笛, 韩烈保, 晁跃辉. 日本结缕草ZjTCP20基因生物信息学及表达模式分析[J]. 草地学报, 2023, 31(2): 321-329.

YANG Zhuo-xiong, DONG Di, HAN Lie-bao, CHAO Yue-hui. Bioinformatics and Expression Pattern Analysis of ZjTCP20 Gene in Zoysia japonica[J]. Acta Agrestia Sinica, 2023, 31(2): 321-329.

0
/ / 推荐

导出引用管理器 EndNote|Ris|BibTeX

链接本文: https://manu40.magtech.com.cn/Jweb_cdxb/CN/10.11733/j.issn.1007-0435.2023.02.003

https://manu40.magtech.com.cn/Jweb_cdxb/CN/Y2023/V31/I2/321

参考文献

[1] MA J,WANG Q,SUN R,et al. Genome-wide Identification and Expression Analysis of TCP Transcription Factors in Gossypium Raimondii[J]. Scientific Reports,2014,4(1):1-10
[2] CUBAS P,LAUTER N,DOEBLEY J,et al. The TCP Aomain:A Motif Found in Proteins Regulating Plant Growth and Development[J]. The Plant Journal,1999,18(2):215-222
[3] NAVAUD O,DABOS P,CARNUS E,et al. TCP Transcription Factors Predate the Emergence of Land Plants[J]. Journal of Molecular Evolution,2007,65(1):23-33
[4] AGUILAR-MARTÍNEZ J A,POZA-CARRIÓN C,CUBAS P. Arabidopsis BRANCHED1 Acts as an Integrator of Branching Signals within Axillary Buds[J]. The Plant Cell,2007,19(2):458-472
[5] 周涛,彭辉,喻望晨,等. 千穗谷TCP基因家族生物信息学分析[J]. 草地学报,2022,30(4):867-878
[6] CRAWFORD B C W,NATH U,CARPENTER R,et al. CINCINNATA Controls Both Cell Differentiation and Growth in Petal Lobes and Leaves of Antirrhinum[J]. Plant Physiology,2004,135(1):244-253
[7] AGUILAR-MARTíNEZ J A,SINHA N J. Analysis of the Role of Arabidopsis Class I TCP Genes AtTCP7,AtTCP8,AtTCP22,and AtTCP23 in Leaf Development[J]. Frontiers in Plant Science,2013(4):406
[8] KIEFFER M,MASTER V,WAITES R,et al. TCP14 and TCP15 Affect Internode Length and Leaf Shape in Arabidopsis[J]. The Plant Journal,2011,68(1):147-158
[9] GUO Z,FUJIOKA S,BLANCAFLOR E B,et al. TCP1 Modulates Brassinosteroid Biosynthesis by Regulating the Expression of the Key Biosynthetic Gene DWARF4 in Arabidopsis Thaliana[J]. Plant Cell,2010,22(4):1161-1173
[10] BALSEMAO-PIRES E,ANDRADE L R,SACHETTO-MARTINS G. Functional Study of TCP23 in Arabidopsis Thaliana During Plant Development[J]. Plant Physiology and Biochemistry,2013,67:120-125
[11] FANG Y,ZHENG Y,LU W,et al. Roles of MiR319-regulated TCPs in Plant Development and Response to Abiotic Stress[J]. The Crop Journal,2021,9(1):17-28
[12] JABBOUR F,NADOT S,DAMERVAL C. Evolution of Floral Symmetry:a State of the Art[J]. Comptes Rendus Biologies,2009,332(2-3):219-231
[13] POZA-CARRION C,AGUILAR-MARTINEZ J A,CUBAS P. Role of TCP Gene BRANCHED1 in the Control of Shoot Branching in Arabidopsis[J]. Plant Signaling & Behavior,2007,2(6):551-552
[14] 安新艳,楼盼盼,郝娟. 植物TCP转录因子的研究进展[J]. 安徽农业学,2020,48(15):20-23,27
[15] KROUK G,LACOMBE B,BIELACH A,et al. Nitrate-regulated Auxin Transport by NRT1.1 Defines a Mechanism for Nutrient Sensing in Plants[J]. Development Cell,2010,18(6):927-937
[16] DANISMAN S,WAL F,DHONDT S,et al. Arabidopsis Class I and ClassⅡTCP Transcription Factors Regulate Jasmonic Acid Metabolism and Leaf Development Antagonistically[J]. Plant Physiology,2012,159(4):1511-1523
[17] GUAN P,WANG R,NACRY P,et al. Nitrate Foraging by Arabidopsis Roots is Mediated by the Transcription Factor TCP20 Through the Systemic Signaling Pathway[J]. Proceedings of the National Academy of Sciences,2014,111(42):15267-15272
[18] 马媛,杨卓雄,董笛,等. 日本结缕草ZjHY5的克隆、亚细胞定位及转录自激活检测[J]. 草地学报,2022,30(3):560-567
[19] 郑荣佳,孔维一,冯嘉欣,等. 结缕草属植物表型多样性分析及优异种质筛选[EB/OL]. https://kns.cnki.net/kcms/detail/detail.aspx?dbcode=CJFD&dbname=CJFDLAST2022&filename=CDXU202209012&uniplatform=NZKPT&v=xve8KgKxj-FOj4UFprEySAK31oAC49kh-zKwlSb0_40xS8oExRqlbBUQ2wT1QRTa,2022-07-22/2022-08-10
[20] HE P,WANG H,YAN Y,et al. Development and Application of a Multiplex Fluorescent PCR for Shigella Detection and Species Identification[J]. Journal of Fluorescence,2022,32(2):707-713
[21] 杨卓雄,韩烈保,晁跃辉,等. 日本结缕草ZjTCP4基因的克隆、转录自激活检测及表达模式分析[J]. 基因组学与应用生物学,2022,41(4):834-842
[22] GASTEIGER E,GATTIKER A,HOOGLAND C,et al. ExPASy:the Proteomics Server for in-depth Protein Knowledge and Analysis[J]. Nucleic acids research,2003,31(13):3784-3788
[23] KELLEY L A,STERNBERG M J. Protein Structure Prediction on the Web:a Case Study Using the Phyre Server[J]. Nature Protocols,2009,4(3):363-371
[24] GARCION C,BéVEN L,FOISSAC X. Comparison of Current Methods for Signal Peptide Prediction in Phytoplasmas[J]. Frontiers in Microbiology,2021(12):661524
[25] 董笛,张宇昕,刘畅,等. 日本结缕草ZjNOL基因的克隆及转录自激活检[J]. 中国草地学报,2019,41(3):15-21
[26] BAILEY T L,WILLIAMS N,MISLEH C,et al. MEME:Discovering and Analyzing DNA and Protein Sequence Motifs[J]. Nucleic Acids Research,2006(34):W369-W373
[27] LETUNIC I,BORK P. Interactive Tree Of Life (iTOL) v5:An Online Tool for Phylogenetic Tree Display and Annotation[J]. Nucleic Acids Research,2021(49):W293-W296
[28] KOKKIRALA V R,YONGGANG P,ABBAGANI S,et al. Subcellular Localization of Proteins of Oryza Sativa L. in the Model Tobacco and Tomato Plants[J]. Plant Signaling & Behavior,2010,5(11):1336-1341
[29] 位志坤,许自成,贾国涛,等. bHLH转录因子家族调控植物非生物逆境胁迫响应的研究进展[EB/OL]. https://kns.cnki.net/kcms/detail/detail.aspx?dbcode=CAPJ&dbname=CAPJLAST&filename=FZZW20220613006&uniplatform=NZKPT&v=zovg2PWufFkNdVr4f_eEZXIDG-#L_AezrzKNO9ulX8tUCYPjZaP9_dXILlJioA,2022-06-15/2022-08-10
[30] 周丽霞,赵志浩,杨蒙迪,等. 油棕TCP转录因子家族生物信息学鉴定及基因表达分析[J]. 分子植物育种,2022,20(17):5637-5648
[31] 冯建英,李立芹,李佳皓，等. 马铃薯TCP家族转录因子鉴定与表达模式分析[J]. 基因组学与应用生物学,2021,40(Z2):2756-2764
[32] 兰婧秋,秦跟基. ClassⅡTCP转录因子的主要功能和分子调控机制[J]. 中国科学:生命科学,2021,51(11):1542-1557
[33] 郭一冉. 分子系统发育分析的生物信息学方法[J]. 山西农经,2016(12):103-104

日本结缕草ZjTCP20基因生物信息学及表达模式分析

Bioinformatics and Expression Pattern Analysis of ZjTCP20 Gene in Zoysia japonica

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	冯冠楠, 安聪, 丁鋆嘉, 张夏香, 庄黎丽. 狗尾草GASA基因家族鉴定及其在不同逆境下表达模式分析[J]. 草地学报, 2022, 30(6): 1379-1387.
[2]	杜雨, 刘筠, 胡晓桐, 贾西贝, 柳迪, 马彦军. 黑果枸杞GRF转录因子家族鉴定与生物信息学分析[J]. 草地学报, 2022, 30(6): 1403-1412.
[3]	李莉萍, 李旭漉, 张晓捷, 谷丽丽, 张博. 紫花苜蓿atpA基因的克隆及表达模式分析[J]. 草地学报, 2022, 30(6): 1413-1421.
[4]	马媛, 杨卓雄, 董笛, 李舒文, 晁跃辉, 许立新. 日本结缕草ZjHY5的克隆、亚细胞定位及转录自激活检测[J]. 草地学报, 2022, 30(3): 560-567.
[5]	吴婧, 范菠菠, 张学峰, 宋金微, 房永雨, 于卓, 闫秀秀, 赵彦, 马艳红. 蒙古冰草ERF转录因子生物信息学及其表达分析[J]. 草地学报, 2022, 30(11): 2910-2921.
[6]	易丹, 王博, 段慧荣, 李毅, 王丽蓉. 白刺14-3-3基因家族的鉴定及表达分析[J]. 草地学报, 2021, 29(3): 443-456.
[7]	方志红, 刘建宁, 张燕, 郭璞, 池惠武, 吴欣明, 贾会丽, 李莲芬, 王运琦, 石永红. 白羊草NAC转录因子家族生物信息学分析[J]. 草地学报, 2020, 28(5): 1266-1274.
[8]	梁雨舟, 张艳, 侯洁茹, 彭燕, 张新全. 白三叶HD-Zip类转录因子TrATHB-12的克隆及表达分析[J]. 草地学报, 2020, 28(2): 319-327.
[9]	滕珂, 檀鹏辉, 范希峰, 岳跃森, 姜红岩, 武菊英. 日本结缕草衰老叶片全长cDNA文库构建及酵母单杂交筛选验证[J]. 草地学报, 2019, 27(6): 1486-1493.
[10]	李春艳, 董洁, 钟华, 董宽虎. 白羊草叶片和根系干旱胁迫下microRNAs差异表达分析[J]. 草地学报, 2019, 27(3): 539-546.
[11]	肖晓琳, 王凯, 张兵, 刘建秀. 沟叶结缕草蛋白质二硫键异构酶(ZmPDIs)基因家族耐盐功能分析[J]. 草地学报, 2018, 26(5): 1181-1189.
[12]	吴榕, 郑国华, 郝玉兰. 白三叶化感作用相关基因CHS的克隆及生物信息学分析[J]. 草地学报, 2017, 25(1): 100-106.
[13]	敖特根白音, 高立杰, 张树华, 云锦凤, 郎明林, 杨学举. 蒙古冰草肌动蛋白基因(MwACT2)克隆与表达分析[J]. 草地学报, 2016, 24(1): 129-136.
[14]	陈卫卫, 唐然, 吴昱煜, 李菲, 张向前, 解新明. 象草PAL基因克隆及其蛋白质结构与功能预测[J]. 草地学报, 2016, 24(1): 137-145.
[15]	张丽君, 刘龙龙, 乔治军, 马名川, 周建萍, 范银燕, 崔林. 燕麦核不育雄蕊转录组测序及分析[J]. , 2014, 22(2): 352-358.