黑果枸杞GRF转录因子家族鉴定与生物信息学分析

doi:10.11733/j.issn.1007-0435.2022.06.011

草地学报 ›› 2022, Vol. 30 ›› Issue (6): 1403-1412.DOI: 10.11733/j.issn.1007-0435.2022.06.011

• 研究论文 • 上一篇

黑果枸杞GRF转录因子家族鉴定与生物信息学分析

杜雨, 刘筠, 胡晓桐, 贾西贝, 柳迪, 马彦军

甘肃农业大学林学院, 甘肃兰州 730070

收稿日期:2021-11-11 修回日期:2022-01-27 发布日期:2022-07-05
通讯作者: 马彦军,E-mail:lxysys01@126.com
作者简介:杜雨(1994-),女,汉族,河南周口人,硕士研究生,主要从事植物种质资源调查收集、保存与研究,E-mail:Duyu0126@126.com
基金资助:
甘肃省自然科学基金（20JR10RA530）；国家自然科学基金（31760244，31560215）资助

Identification and Bioinformatic Analysis of GRF Transcription Factor Family in Lycium ruthenicum Murr.

DU Yu, LIU Yun, HU Xiao-tong, JIA Xi-bei, LIU Di, MA Yan-jun

College of Fovestry, Gansu Agricultural University, Lanzhou, Gansu Province 730070, China

Received:2021-11-11 Revised:2022-01-27 Published:2022-07-05

摘要/Abstract

摘要： 为探究黑果枸杞（Lycium ruthenicum） GRF基因家族成员在盐胁迫下的耐盐分子机制，采用生物信息学方法，对黑果枸杞转录组数据库进行分析，并对GRF家族成员的理化性质、保守结构域、进化树、基因家族表达谱进行分析。结果表明，通过转录组数据共筛选出14个GRF家族成员，这些蛋白编码氨基酸的个数为136~585 aa，等电点为5.58~9.33，分子量为14.566 97~62.986 65 kDa。保守基序表明，多数黑果枸杞GRF的N端具有QLQ和WRC保守结构域。系统进化分析发现，14个LrGRFs被分为7个亚族，位于同一亚族的GRF基序组成相似，部分黑果枸杞GRF与番茄GRF具有相对应的进化关系。表达谱表明，不同组织的LrGRFs在不同盐胁迫下均有响应，其中LrGRF6，LrGRF10和LrGRF12在根中高表达，而LrGRF2，LrGRF6和LrGRF10在叶中表现为高表达，这表明其可能在黑果枸杞中发挥着更加重要的调控作用。结果为进一步深入揭示LrGRFs在盐胁迫环境下的分子机制奠定了理论基础。

关键词: 黑果枸杞, NaCl胁迫, GRF基因家族, 生物信息学, 进化树, 表达谱分析

Abstract: In order to explore the molecular mechanismes of salt tolerance of Lycium ruthenicum GRF gene family members under salt stress,a bioinformatic method was used to analyze the transcriptome database of Lycium ruthenicum. At the same time,we analyzed the physical and chemical properties conserved domains,evolutionary trees,and expression profiles of GRF family members. The results showed that a total of 14 GRF family members were screened in transcriptome data,and the number of amino acids encoded proteins was 136~585 aa,with an isoelectric point of 5.58~9.33 and molecular weight of 14.566 97~62.986 65 kDa. Analysis of conservative motifs found that the most N-terminal of Lycium ruthenicum GRF had QLQ and WRC conserved domains. Phylogenetic analysis found that 14 LrGRFs were divided into 7 subfamilies,and the GRF motif composition in the same subfamily was the similar. Some Lycium ruthenicum GRF and Solanum lycopersicum had corresponding evolutionary relationship. Expression profiles showed that LrGRFs in different tissues responded to different salt stresses. Among them,LrGRF6,LrGRF10 and LrGRF12 were highly expressed in roots,while LrGRF2, LrGRF6 and LrGRF10 were highly expressed in leaves,which indicated that they may play a more important regulatory role in Lycium ruthenicum. The results laid a theoretical foundation for further revealing the molecular mechanism of LrGRFs under salt stress

Key words: Lycium ruthenicum, NaCl stress, GRF gene family, Bioinformatics, Evolutionary tree, Expression analysis

中图分类号:

S722.3+6

杜雨, 刘筠, 胡晓桐, 贾西贝, 柳迪, 马彦军. 黑果枸杞GRF转录因子家族鉴定与生物信息学分析[J]. 草地学报, 2022, 30(6): 1403-1412.

DU Yu, LIU Yun, HU Xiao-tong, JIA Xi-bei, LIU Di, MA Yan-jun. Identification and Bioinformatic Analysis of GRF Transcription Factor Family in Lycium ruthenicum Murr.[J]. Acta Agrestia Sinica, 2022, 30(6): 1403-1412.

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黑果枸杞GRF转录因子家族鉴定与生物信息学分析

Identification and Bioinformatic Analysis of GRF Transcription Factor Family in Lycium ruthenicum Murr.

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