草地早熟禾BBML基因的克隆及表达模式分析

doi:10.11733/j.issn.1007-0435.2024.02.006

草地学报 ›› 2024, Vol. 32 ›› Issue (2): 396-408.DOI: 10.11733/j.issn.1007-0435.2024.02.006

草地早熟禾BBML基因的克隆及表达模式分析

余江弟, 李玉珠, 苗佳敏, 丁菲菲, 白小明, 牛奎举

甘肃农业大学草业学院/草业生态系统教育部重点实验室/中-美草地畜牧业可持续发展研究中心, 甘肃兰州 730070

收稿日期:2023-10-30 修回日期:2023-12-07 出版日期:2024-02-15 发布日期:2024-03-06
通讯作者: 李玉珠,E-mail:liyz@gsau.edu.cn
作者简介:余江弟(1999-),女,汉族,甘肃天水人,硕士研究生,主要从事草类植物分子育种方面研究,E-mail:2605257509@qq.com
基金资助:
草业生态系统教育部重点实验室（甘肃农业大学）2022年开放课题（KLGE202217）；甘肃农业大学科技创新基金（青年导师扶持基金）（GAU-QDFC-2021-02）；草种创新与草地农业生态系统全国重点实验室开放基金课题（SKLHIGA202301）资助

Cloning and Expression Pattern Analysis of BBML Gene in Poa pratensis(Kentucky Bluegrass)

YU Jiang-di, LI Yu-zhu, MIAO Jia-min, DING Fei-fei, BAI Xiao-ming, NIU Kui-ju

College of Pratacultural Science, Gansu Agricultural University;Key Laboratory of Grassland Ecosystem, Ministry of Education;Sino-U. S. Center for Grassland Ecosystem Sustainability, Lanzhou, Gansu Province 730070, China

Received:2023-10-30 Revised:2023-12-07 Online:2024-02-15 Published:2024-03-06

摘要/Abstract

摘要： 为探究婴儿潮（BABY BOOM，BBM）基因诱导草地早熟禾（Poa pratensis）体胚发生的潜在功能，本研究采用同源克隆方法获得PpBBML（PpBBM-like）基因，并对其进行生信分析、亚细胞定位和表达模式分析。结果表明：PpBBML编码的蛋白具BBM特有的bbm-1基序且定位于细胞核，并与小麦（Triticum aestivum） BBM亲缘关系最近。PpBBML的表达水平存在组织特异性，表达量为花药>茎基>幼叶>根茎>老叶>不定根。6-BA，NAA （除0 h外），MeJA和EBR （除4 h外）处理后的PpBBML表达量均高于CK；GA₃处理后的PpBBML表达量均低于CK。在仅添加生长素的愈伤组织诱导培养基上，其表达量在接种第2 d最高。综上所述，PpBBML是AP2亚家族的转录因子基因，其表达量在幼嫩组织中最高且受到不同激素的诱导，并在愈伤组织形成初期表达量急剧上升，因此，该基因可能具有诱导草地早熟禾胚性愈伤组织发生的潜力。

关键词: 草地早熟禾, BBM基因, 基因克隆, 生物信息学分析, 表达模式分析

Abstract: To explore the potential function of the BABY BOOM (BBM) gene in inducing somatic embryogenesis in Poa pratensis. The PpBBML (PpBBM-like) gene was obtained through homologous cloning and analyzed using bioinformatics,subcellular localization,and expression pattern analysis. The results showed that the protein encoded by PpBBML had the unique bbm-1 motif of BBM and was located in the nucleus,and was closely related to wheat (Triticum aestivum) BBM. The expression level of the PpBBML gene exhibits tissue specificity,with the highest expression in anthers,followed by stem base,young leaves,rhizomes,old leaves,and adventitious roots. The expression levels of PpBBML after treatment with 6-BA,NAA (except 0 h),MeJA,and EBR (except 4 h) were higher than those of the control;however,the expression of PpBBML after GA₃ treatment was lower than that of the control. In callus induction medium supplemented only with auxin,the expression of PpBBML peaked on the second day of inoculation. In summary,PpBBML is a transcription factor gene of the AP2 subfamily.Its expression is the highest in young tissues,is induced by different hormones,and increases sharply in the early stage of callus formation. Therefore,this gene may have the potential to induce embryonic callus formation in Poa pratensis.

Key words: Kentucky Bluegrass, BBM gene, Gene cloning, Bioinformatics analysis, Expression pattern analysis

中图分类号:

S688.4

余江弟, 李玉珠, 苗佳敏, 丁菲菲, 白小明, 牛奎举. 草地早熟禾BBML基因的克隆及表达模式分析[J]. 草地学报, 2024, 32(2): 396-408.

YU Jiang-di, LI Yu-zhu, MIAO Jia-min, DING Fei-fei, BAI Xiao-ming, NIU Kui-ju. Cloning and Expression Pattern Analysis of BBML Gene in Poa pratensis(Kentucky Bluegrass)[J]. Acta Agrestia Sinica, 2024, 32(2): 396-408.

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草地早熟禾BBML基因的克隆及表达模式分析

Cloning and Expression Pattern Analysis of BBML Gene in Poa pratensis(Kentucky Bluegrass)

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