新麦草独脚金内酯合成相关基因CCD7的克隆及表达分析

doi:10.11733/j.issn.1007-0435.2025.02.006

草地学报 ›› 2025, Vol. 33 ›› Issue (2): 382-390.DOI: 10.11733/j.issn.1007-0435.2025.02.006

• 研究论文 • 上一篇

新麦草独脚金内酯合成相关基因CCD7的克隆及表达分析

艾芊¹, 云岚^1,2, 任晓敏¹, 姚娜¹

1. 内蒙古农业大学草业学院, 内蒙古呼和浩特 010018;
2. 草地资源教育部重点实验室, 内蒙古呼和浩特 010011

收稿日期:2024-03-25 修回日期:2024-05-20 发布日期:2025-03-01
通讯作者: 云岚,E-mail:yunlan@imau.edu.cn
作者简介:艾芊（1999-）,男,汉族,云南昆明人,博士研究生,主要从事牧草种质资源与遗传育种研究,E-mail:1620814079@qq.com
基金资助:
内蒙古自治区自然科学基金重点项目（2023ZD07）;国家自然科学基金面上项目（32371762）;内蒙古自治区种业创新重大示范工程“揭榜挂帅”项目（2022-JBGS00400303）资助

Cloning and Expression Analysis of CCD7 Gene Related to Strigolactone Synthesis inPsathyrostachys juncea

AI Qian¹, YUN Lan^1,2, REN Xiao-min¹, YAO Na¹

1. College of Grassland Resource and Environment, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia 010018, China;
2. Key Laboratory of Grassland Resources of Education Ministry, Hohhot, Inner Mongolia 010011, China

Received:2024-03-25 Revised:2024-05-20 Published:2025-03-01

摘要/Abstract

摘要： 植物CCD7基因参与合成独脚金内酯。为研究新麦草[Psathyrostachys juncea （Fisch.）Nevski]CCD7基因的功能,本研究以多分蘖型新麦草和少分蘖型新麦草的分蘖节为材料,通过RNA-seq及荧光定量PCR分析CCD7基因在新麦草中的表达量,通过构建pcambia1300-cYFP-CCD7过表达载体,分析新麦草CCD7基因在烟草叶片中的亚细胞定位；通过生物信息学分析预测CCD7基因的基本功能。结果显示,新麦草CCD7基因序列克隆全长为1638 bp,亚细胞定位新麦草CCD7基因在烟草叶片细胞的叶绿体中表达；表达量分析表明新麦草的分蘖数量与该基因的表达量呈现负相关关系。预测新麦草CCD7保守结构域的范围是第13到第544个氨基酸,该基因在9个近缘物种中功能保守,编码蛋白性质相近,新麦草CCD7蛋白的丝氨酸磷酸化位点最有可能是蛋白发挥功能的磷酸化位点。

关键词: 新麦草, CCD7, 过表达载体, 亚细胞定位, 基因克隆

Abstract: Plant CCD7 gene is involved in the synthesis of strigolactones. In order to study the function of CCD7 gene in Psathyrostachys juncea, the tillering nodes of multi-tillering Psathyrostachys juncea and less-tillering P. juncea were used as materials. The expression of CCD7 gene in P. juncea was analyzed by RNA-seq and fluorescence quantitative PCR. The subcellular localization of CCD7 gene in tobacco leaves was analyzed by constructing pcambia 1300-cYFP-CCD7 overexpression vector. The basic functions of CCD7 gene were predicted by bioinformatics analysis.The results showed that the full length of P. juncea CCD7 gene sequence was 1 638 bp, and the subcellular localization of P. junceaCCD7 gene test found it was expressed in the chloroplast of tobacco leaf cells. The expression analysis showed that the tiller number of P. juncea was negatively correlated with the high expression of the gene. It was predicted that the conserved domain of CCD7 in P. juncea ranged from 13 to 544 amino acids. The gene was conserved in 9 closely related species, and the protein properties were similar. The serine phosphorylation site of CCD7 protein in P. juncea was most likely to be the phosphorylation site of protein function.

Key words: Psathyrostachys juncea, CCD7, Over expression vector, subcellular localization, Gene cloning

中图分类号:

S543.9

艾芊, 云岚, 任晓敏, 姚娜. 新麦草独脚金内酯合成相关基因CCD7的克隆及表达分析[J]. 草地学报, 2025, 33(2): 382-390.

AI Qian, YUN Lan, REN Xiao-min, YAO Na. Cloning and Expression Analysis of CCD7 Gene Related to Strigolactone Synthesis inPsathyrostachys juncea[J]. Acta Agrestia Sinica, 2025, 33(2): 382-390.

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新麦草独脚金内酯合成相关基因CCD7的克隆及表达分析

Cloning and Expression Analysis of CCD7 Gene Related to Strigolactone Synthesis inPsathyrostachys juncea

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