狗尾草GASA基因家族鉴定及其在不同逆境下表达模式分析

doi:10.11733/j.issn.1007-0435.2022.06.008

草地学报 ›› 2022, Vol. 30 ›› Issue (6): 1379-1387.DOI: 10.11733/j.issn.1007-0435.2022.06.008

• 研究论文 • 上一篇

狗尾草GASA基因家族鉴定及其在不同逆境下表达模式分析

冯冠楠, 安聪, 丁鋆嘉, 张夏香, 庄黎丽

南京农业大学, 江苏南京 210095

收稿日期:2022-01-14 修回日期:2022-03-30 发布日期:2022-07-05
通讯作者: 庄黎丽,E-mail:nauzll@njau.edu.cn
作者简介:冯冠楠(1997-),男,汉族,山西阳泉人,学士,主要从事草类植物抗逆研究,E-mail:fgn710518@sjtu.edu.cn
基金资助:
国家自然科学基金面上项目（32171689）资助

Characterization and Analysis of GASA Gene Family and Response to Abiotic Stress in Setaria viridis

FENG Guan-nan, AN Cong, DING Yun-jia, ZHANG Xia-xiang, ZHUANG Li-li

Nanjing Agricultural University, Nanjing, Jiangsu Province 210095, China

Received:2022-01-14 Revised:2022-03-30 Published:2022-07-05

摘要/Abstract

摘要： GASA蛋白（Gibberellic acid-stimulated in arabidopsis）是植物特有的小分子蛋白，通过参与调控相关植物激素信号转导与生长发育过程在植物体内发挥着重要作用。深入研究GASA蛋白的功能对阐明植物小分子蛋白作用机理具有重要理论研究意义。狗尾草（Setaria viridis）是新型的C₄模式植物，对其GASA基因家族的研究尚未见报道。本研究采用生物信息学技术，基于狗尾草全基因组序列，分析获得12个GASA基因。进一步生物信息学分析结果表明，狗尾草GASA家族基因结构简单，GASA蛋白可划分为三个进化枝，均含有3~4个模体结构，12个基因启动子区共同含有响应脱水、低温、高盐及植物激素的顺式作用元件。采用实时荧光定量PCR技术对短期干旱（15%聚乙二醇6000）、冷害（4℃）、盐害（150 mM NaCl）逆境胁迫下狗尾草叶片中GASA基因表达模式进行了初步研究。结果表明，狗尾草不同GASA基因对各类逆境胁迫有响应。研究结果将为进一步开展GASA基因参与逆境胁迫的功能解析提供初步的实验证据。

关键词: GASA蛋白, 生物信息学分析, 逆境胁迫, 表达模式

Abstract: GASA (Gibberellic acid-stimulated in arabidopsis) is a class of plant-specific small molecule proteins that plays crucial roles in regulating the growth and development of plant and related hormone signal transduction. It is of great theoretical significance to elucidate the function of GASA protein to reveal the mechanism of the regulatory network of plant small molecular protein. Setaria viridis is a new C₄ model plant,and the study of GASA gene family in Setaria viridis has not been reported. This article obtained 12 GASA in Setaria viridis based on the whole genome sequence. The genetic structure,protein structure,and phylogeny of GASA gene family were analyzed. Results showed that most GASA proteins contained three to four motifs and were divided into three clades. Bioinformatic analysis showed that promoters of GASA family members contained common cis-elements like dehydration response elements,low-temperatures response elements,high salinity stress responsiveness elements,etc. Quantitative real-time PCR was adopted to assess the expression pattern of GASA gene in leaves of Setaria viridis under short-term drought (15% PEG6000),cold (4℃),and salt stress (150 mM NaCl). Results showed that all genes could respond variously to different abiotic stress. The results of this study will provide experimental evidence for further functional analysis of GASA genes involved in abiotic stresses.

Key words: GASA protein, Bioinformatic analysis, Abiotic stress, Expression pattern

中图分类号:

Q344+.13

冯冠楠, 安聪, 丁鋆嘉, 张夏香, 庄黎丽. 狗尾草GASA基因家族鉴定及其在不同逆境下表达模式分析[J]. 草地学报, 2022, 30(6): 1379-1387.

FENG Guan-nan, AN Cong, DING Yun-jia, ZHANG Xia-xiang, ZHUANG Li-li. Characterization and Analysis of GASA Gene Family and Response to Abiotic Stress in Setaria viridis[J]. Acta Agrestia Sinica, 2022, 30(6): 1379-1387.

0
/ / 推荐

导出引用管理器 EndNote|Ris|BibTeX

链接本文: https://manu40.magtech.com.cn/Jweb_cdxb/CN/10.11733/j.issn.1007-0435.2022.06.008

https://manu40.magtech.com.cn/Jweb_cdxb/CN/Y2022/V30/I6/1379

参考文献

[1] SILVERSTEIN K A,MOSKAL W A Jr,WU H C,et al. Small cysteine-rich peptides resembling antimicrobial peptides have been under-predicted in plants[J]. The Plant Journal,2007,51(2):262-280
[2] BEN-NISSAN G,LEE J Y,BOROHOV A,et al. GIP,a Petunia hybrida GA-induced cysteine-rich protein:a possible role in shoot elongation and transition to flowering[J]. The Plant Journal,2010,37(2):229-238
[3] ZHANG S C,WANG X J. Expression pattern of GASA,downstream genes of DELLA,in Arabidopsis[J]. Chinese Science Bulletin,2008,53(24):3839-3846
[4] LI W,ZHEN W,XU Y,et al. OsGSR1 is involved in crosstalk between gibberellins and brassinosteroids in rice[J]. The Plant Journal,2009,57(3):498-510
[5] HOU D,BAI Q,LI J,et al. The gibberellic acid-stimulated transcript gene family in Moso Bamboo:A genome-wide survey and expression profiling during development and abiotic stresses[J]. Journal of Plant Growth Regulation,2018,37:1135-1147
[6] 吕亮杰,陈希勇,张业伦,等. 小麦GASA基因家族生物信息学分析[J]. 作物杂志,2008,6:58-67
[7] ZIMMERMANN R,SAKAI H,HOCHHOLDINGER F. The gibberellic acid stimulated-like gene family in maize and its role in lateral root development[J]. Plant Physiology,2010,152(1):356-365
[8] ROXRUD I,LID S E,FLETCHER J C,et al. GASA4,one of the 14-member Arabidopsis GASA family of small polypeptides,regulates flowering and seed development[J]. Plant and Cell Physiology,2007,48(3):471483
[9] RUBINOVICH L,WERISS D. The Arabidopsis cysteine-rich protein GASA4 promotes GA responses and exhibits redox activity in bacteria and in planta[J]. Plant Journal,2010,64(6):1018-1027
[10] MOYANO-CAÑETE E,BELLIDO M L,GARCÍA-CAPARRÒS N,et al. FaGAST2,a strawberry ripening-related gene,acts together with FaGAST1 to determine cell size of the fruit receptacle[J]. Plant & Cell Physiology,2013,54(2):218-236
[11] CHEONG J J,LEE G H,KWON H B. Expression and regulation of the RSI-1 gene during lateral root initiation[J]. Journal of Plant Biology,1999,42(4):259-265
[12] RUBINOVICH L,RUTHSTEIN S,WEISS D. The Arabidopsis cysteine-rich GASA5 is a redox-active metalloprotein that suppresses gibberellin responses[J]. Molecular Plant,2014,7(1):244-247
[13] SUN S,WANG H,YU H,et al. GASA14 regulates leaf expansion and abiotic stress resistance by modulating reactive oxygen species accumulation[J]. Journal of Experimental Botany,2013,64(6):1637-1647
[14] ZHANG S,YANG C,PENG J,et al. GASA5,a regulator of flowering time and stem growth in Arabidopsis thaliana[J]. Plant Molecular Biology,2009,69(6):745-759
[15] FURUKAWA T,SAKAGUCHI N,SHINADA H. Two OsGASR genes,rice GAST homologue genes that are abundant in proliferating tissues,show different expression patterns in developing panicles[J]. Genes & Genetic Systems,2006,81(3):171-180
[16] AUBERT D,CHEVILLARD M,DOME A M,et al. Expression patterns of GASA genes in Arabidopsis thaliana:the GASA4 gene is up-regulated by gibberellins in meristematic regions[J]. Plant Molecular Biology,1998,36(6):871-883
[17] HAN S,JIAO Z Y,NIU M X,et al. Genome-wide comprehensive analysis of the GASA gene family in Populus[J]. International Journal of Molecular Sciences,2021,22:12336
[18] SHABAN M,KHAN A H,NOOR E,et al. Genome-wide dissection,characterization,and expression profiling of cotton GASA genes reveal their importance in regulating abiotic stresses[J]. International Journal of Agriculture and Biology,2020,25:181-190
[19] BALAJI V,SMART C D. Over-expression of snakin-2 and extensin-like protein genes restricts pathogen invasiveness and enhances tolerance to Clavibacter michiganensis subsp. michiganensis in transgenic tomato (Solanum lycopersicum)[J]. Transgenic Research,2012,21(1):23-37
[20] BINDSCHEDLER L V,DEWDNEY J,BLEE K A,et al. Peroxidase-dependent apoplastic oxidative burst in Arabidopsis required for pathogen resistance[J]. Plant Journal for Cell & Molecular Biology,2006,47(6):851-863
[21] BERROCAL L M,SEGURA A,MORENO M,et al. Snakin-2,an antimicrobial peptide from potato whose gene is locally induced by wounding and responds to pathogen infection[J]. Plant Physiology,2002,128:951-961
[22] KO C B,WOO Y M,LEE D J,et al. Enhanced tolerance to heat stress in transgenic plants expressing the GASA4 gene[J]. Plant Physiology and Biochemistry,2006,45:722-728
[23] 李昆仑. 碱胁迫应答GsGASA1及GsGASA2基因表达特性研究[J]. 东北农业大学学报,2012,43(1):143-148
[24] ALONSO-RAMIREZ A,RODRÍGUEZ D,REYES D,et al. Evidence for a role of gibberellins in salicylic acid-modulated early plant responses to abiotic stress in Arabidopsis seeds[J]. Plant Physiology,2009,150:1335-1344
[25] LEE S C,HAN S K,KIM S R. Salt-and ABA-inducible OsGASR1 is involved in salt tolerance[J]. Journal of Plant Biology,2015,58(2):96-101
[26] THOMAS P B,LIN Wang,KERRY Swartwood,et al. Setaria viridis:A model for C₄ photosynthesis[J]. The Plant Cell,2010,22:2537-2544
[27] 冯文新,宗毓铮,郝兴宇,等. 高CO₂浓度下狗尾草与谷子光合特性比较研究[J]. 草地学报,2017,25(2):424-428
[28] 赵辉,郭静远,孔华,等. 狗尾草胚性愈伤高效农杆菌转化体系的建立[J]. 热带作物学报,2017,38(6):129-135
[29] 王燕,张礼宁,唐方毅,等. 柳枝稷GRF转录因子家族全基因组鉴定与分析[J]. 草地学报,2022,30(3):575-586
[30] 易丹,王博,段慧荣,等. 白刺14-3-3基因家族的鉴定及表达分析[J]. 草地学报,2021,29(3):443-456
[31] 李相伯,史双月,朱韵,等. 植物GAST家族基因功能研究进展[J]. 扬州大学学报(农业与生命科学版),2019,40(2):8
[32] LAMBRET-FROTTÉJ,DE ALMEIDA L C S,DE MOURA S M,et al. Validating internal control genes for the accurate normalization of qPCR expression analysis of the novel model plant Setaria viridis[J]. Plos One,2015,10(8):e0135006
[33] SHI L,GAST RT,GOPALRAJ M,et al. Characterization of a shoot-specific,GA3-and ABA-regulated gene from tomato[J].The Plant Journal,1992,2(2):153-159
[34] FURUKAWA T,SAKAGUCHI N,SHIMADA H. Two OsGASR genes,rice GAST homologue genes that are abundant in proliferating tissues,show different expression patterns in developing panicles[J]. Genes & Genetic Systems,2006,81(3):171-180

狗尾草GASA基因家族鉴定及其在不同逆境下表达模式分析

Characterization and Analysis of GASA Gene Family and Response to Abiotic Stress in Setaria viridis

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 10

编辑推荐

Metrics

本文评价

[1]	易丹, 王博, 段慧荣, 李毅, 王丽蓉. 白刺14-3-3基因家族的鉴定及表达分析[J]. 草地学报, 2021, 29(3): 443-456.
[2]	梁雨舟, 张艳, 侯洁茹, 彭燕, 张新全. 白三叶HD-Zip类转录因子TrATHB-12的克隆及表达分析[J]. 草地学报, 2020, 28(2): 319-327.
[3]	贾会丽, 石永红, 王学敏, 王运琦, 吴欣明, 刘建宁, 方志红, 张燕, 董宽虎. 紫花苜蓿MsLEA4启动子的克隆及表达分析[J]. 草地学报, 2019, 27(4): 789-796.
[4]	方志红, 崔苗苗, 张燕, 任彬琳, 石永红, 刘建宁, 王运琦, 董宽虎, 高洪文, 吴欣明. 紫花苜蓿MsSOS1基因的克隆与表达分析[J]. 草地学报, 2018, 26(6): 1479-1489.
[5]	肖晓琳, 王凯, 张兵, 刘建秀. 沟叶结缕草蛋白质二硫键异构酶(ZmPDIs)基因家族耐盐功能分析[J]. 草地学报, 2018, 26(5): 1181-1189.
[6]	吴榕, 郑国华, 郝玉兰. 白三叶化感作用相关基因CHS的克隆及生物信息学分析[J]. 草地学报, 2017, 25(1): 100-106.
[7]	敖特根白音, 高立杰, 张树华, 云锦凤, 郎明林, 杨学举. 蒙古冰草肌动蛋白基因(MwACT2)克隆与表达分析[J]. 草地学报, 2016, 24(1): 129-136.
[8]	张丽君, 刘龙龙, 乔治军, 马名川, 周建萍, 范银燕, 崔林. 燕麦核不育雄蕊转录组测序及分析[J]. , 2014, 22(2): 352-358.
[9]	闫慧芳, 毛培胜, 夏方山. 植物抗氧化剂谷胱甘肽研究进展[J]. , 2013, 21(3): 428-434.
[10]	杨锦芬, 郭振飞. 柱花草SgNCED1基因的克隆及功能分析[J]. , 2006, 14(3): 298-300.