草地学报 ›› 2023, Vol. 31 ›› Issue (3): 668-675.DOI: 10.11733/j.issn.1007-0435.2023.03.006

• 研究论文 • 上一篇    下一篇

切根对5龄老芒麦根系土壤养分和微生物生物量及C,N,P化学计量特征的影响

李霞, 祁娟, 路欣, 独双双, 贾燕伟, 赛宁刚   

  1. 甘肃农业大学草业学院, 草业生态系统教育部重点实验室, 甘肃省草业工程实验室, 中-美草地畜牧业可持续研究中心, 甘肃 兰州 730070
  • 收稿日期:2022-08-22 修回日期:2022-10-27 出版日期:2023-03-15 发布日期:2023-04-01
  • 通讯作者: 祁娟,E-mail:qijuan@gsau.edu.cn
  • 作者简介:李霞(1997-),女,汉族,甘肃武威人,硕士研究生,主要从事草种质资源与植物生理生态研究,E-mail:2445266238@qq.com
  • 基金资助:
    国家自然科学基金(31660684);国家现代农业产业技术体系(CARS-34);青海省青藏高原优良牧草种质资源利用重点实验室(2020-ZJ-Y03)项目资助

Effects of Root Cutting on Nutrient Content, Microbial Biomass and Stoichiometric Characteristics of C, N, P in Rhizosphere Soil of 5-year-old Elymus sibiricus

LI Xia, QI Juan, LU Xin, DU Shuang-shuang, JIA Yan-wei, SAI Ning-gang   

  1. College of Pratacultural Science, Gansu Agricultural University;Key Laboratory of Grassland Ecosystem, Ministry of Education;Gansu Grassland Engineering Laboratory;China-US Center for Grazing Land Ecosystem Sustainability, Lanzhou, Gansu Province 730070, China
  • Received:2022-08-22 Revised:2022-10-27 Online:2023-03-15 Published:2023-04-01

摘要: 本文以5龄老芒麦(Elymus sibiricus L.)为研究对象,采用分光光度计和氯仿熏蒸等方法测定土壤养分和微生物生物量,探讨了“一”字切根(RT1)和“十”字切根(RT2)处理对老芒麦根系土壤养分和微生物生物量及C,N,P化学计量特征的影响。结果表明:除土壤pH和速效钾外,2种切根处理均能改善土壤养分和提高微生物生物量碳氮磷含量,其微生物生物量碳和氮含量在RT1处理下增加更显著。同时,切根处理使土壤碳氮比、碳磷比、微生物量氮磷比增加,氮磷比、微生物量碳氮比降低,表明切根处理可以缓解老芒麦生长过程中受土壤N,P元素的限制。相关性分析表明,土壤有机碳和全氮与微生物量碳、氮和微生物量氮磷比显著正相关,土壤碳磷比与微生物量磷和氮显著正相关。2种切根处理均改善了老芒麦根系土壤环境,为老芒麦人工草地的科学管理提供理论依据。

关键词: 切根, 老芒麦, 土壤养分, 微生物生物量, 化学计量特征

Abstract: In this paper, we measured the rhizosphere soil nutrients, microbial biomass, and carbon (C), nitrogen (N) and phosphorus (P) content of 5-year-old Elymus sibiricus based on spectrophotometer and chloroform fumigation, to compare two root cutting treatments, "one-line" (RT1) and "cross" (RT2).The results showed that in addition to soil pH and available potassium, the root-cutting treatments could both improve soil nutrient content, microbial biomass, and contents of C, N, and P, and RT1 received significantly more microbial biomass and contents of C and N. Meanwhile, root-cutting treatments increased C/N and C/P in soil and microbial N/P, while soil N/P and microbial C/N were decreased. It indicated that root-cutting treatments could alleviate the limitation of soil N and P during the growth of E. sibiricus. Correlation analysis showed that soil organic carbon and total nitrogen were significantly positively correlated with microbial C, N, and N/P ratio, while soil C/P ratio was significantly positively correlated with microbial P and N. Two root cutting treatments improved the rhizosphere soil environment in the 5-year-old E. sibiricus, which provides theoretical basis for scientific management of E. sibiricus artificial grassland.

Key words: Root cutting, Elymus sibiricus, Soil nutrients, Microbial biomass, Stoichiometric characteristics

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