草地学报

草地学报 ›› 2023, Vol. 31 ›› Issue (2): 456-463.DOI: 10.11733/j.issn.1007-0435.2023.02.019

• 研究论文 • 上一篇    

外源赤霉素和吲哚乙酸对老芒麦生长、生理特性及染色体端粒的调控

贾燕伟, 祁娟, 赛宁刚, 独双双, 路欣, 李霞   

  1. 甘肃农业大学草业学院, 草业生态系统教育部重点实验室, 甘肃省草业工程实验室, 中-美草地畜牧业可持续研究中心, 甘肃 兰州 730070
  • 收稿日期:2022-09-16 修回日期:2022-10-25 发布日期:2023-02-28
  • 通讯作者: 祁娟,E-mail:qijuan@gsau.edu.cn
  • 作者简介:贾燕伟(1996-),女,汉族,甘肃会宁人,硕士研究生,主要从事草种质资源与植物生理生态研究,E-mail:3093616509@qq.com
  • 基金资助:
    国家自然科学基金(31660684)；甘肃省河西灌区人工草地碳储量及碳汇价值评估(kjcx2022009)；国家现代农业产业技术体系(CARS-34)资助

Regulation of Exogenous GA3 and IAA on Growth,Physiological Properties and Chromosomal Telomeres of Elymus sibiricus

JIA Yan-wei, QI Juan, SAI Ning-gang, DU Shuang-shuang, LU Xin, LI Xia   

  1. College of Pratacultural Science, Gansu Agricultural University;Key Laboratory of Grassland Ecosystem, Ministry of Education;Gansu Grassland Engineering Laboratory;China-US Center for Grazing Land Ecosystem Sustainability, Lanzhou, Gansu Province 730070, China
  • Received:2022-09-16 Revised:2022-10-25 Published:2023-02-28

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摘要： 本研究以'川草2号'老芒麦(Elymus sibiricus 'Chuancao No.2')为材料，采用砂培法，通过测定幼苗形态、生理特性和端粒酶活性，研究喷施不同浓度(30，75和150 mg·L-1)赤霉素(Gibberellin，GA3)和吲哚乙酸(Indole acetic acid，IAA)后对老芒麦幼苗生长发育过程的影响，以期为促进老芒麦生长、提高产量甚至防止早衰提供理论依据。结果表明，各处理提高了株高、叶长、叶面积和地上生物量，150 mg·L-1 GA3和30 mg·L-1IAA处理利于老芒麦植株生长和生物量积累。GA3各处理降低了10~15 d的叶绿素含量和第15 d的丙二醛(Malondialdehyde，MDA)含量，显著提高了0~5 d的超氧化物歧化酶(Superoxide dismutase，SOD)活性、0~15 d的过氧化物酶(Peroxidase，POD)活性、第10 d过氧化氢酶(Catalase，CAT)活性及第15 d的端粒酶活性。IAA各处理显著提高了10~15 d的可溶性蛋白含量，提高了0~5 d的SOD活性及0~15 d的POD活性。主成分综合评价得出，150 mg·L-1 GA3处理效果优于30和75 mg·L-1 GA3及30，75和150 mg·L-1 IAA处理效果。

关键词: 老芒麦, 外源激素, 生长发育, 形态特征, 生理特性, 端粒酶活性

Abstract: The effects of different concentrations (30,75 and 150 mg·L-1) of gibberellin (GA3) and indole acetic acid (IAA) on the growth and development of Elymus sibiricus seedlings were investigated by measuring the morphological and physiological properties,and telomerase activity of the seedlings,with a view to providing a theoretical basis for promoting the growth and development of E. sibiricus,increasing the yield and even preventing premature failure. The E. sibiricus 'Chuancao No.2' was used as the experimental material by using the sand culture method. The results showed that the treatments increased plant height,leaf length,leaf area and above-ground biomass,and 150 mg·L-1 of GA3 and 30 mg·L-1 of IAA treatments were beneficial to plant growth and biomass accumulation of E. sibiricus. The GA3 treatments reduced chlorophyll content on days 10 to 15 and malondialdehyde (MDA) content on day 15,and significantly increased superoxide dismutase (SOD) activity on days 0 to 5,peroxidase (POD) activity on days 0 to 15,catalase (CAT) activity on day 10 and telomerase activity on day 15. The IAA treatments significantly increased the soluble protein content on days 10 to 15,SOD activity on days 0 to 5 and POD activity on days 0 to 15. According to comprehensive evaluation of principal components,the effect of 150 mg·L-1 GA3 treatment was superior to other treatments.

Key words: Elymus sibiricus, Exogenous hormone, Growth and development, Morphological characteristics, Physiological properties, Telomerase activity

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