青海草原毛虫幼虫感器类型及GqinOBPs表达分析

doi:10.11733/j.issn.1007-0435.2023.12.007

草地学报 ›› 2023, Vol. 31 ›› Issue (12): 3626-3635.DOI: 10.11733/j.issn.1007-0435.2023.12.007

青海草原毛虫幼虫感器类型及GqinOBPs表达分析

南彦斌, 王克鑫, 潘学能, 何啟月, 刘占玲, 周渊涛

青海大学农牧学院, 青海西宁 810016

收稿日期:2023-07-10 修回日期:2023-10-17 出版日期:2023-12-15 发布日期:2024-01-03
通讯作者: 周渊涛,E-mail:1282186715@qq.com
作者简介:南彦斌(1997-)，男，汉族，甘肃会宁人，硕士研究生，主要从事昆虫化学生态学研究，E-mail:3028356591@qq.com
基金资助:
青海省科技厅青年基金项目；草原毛虫嗅觉感受分子机理的研究(2022-ZJ-949Q)资助

Analysis of Larval Sensor Structure and GqinOBPs Expression of Gynaephora qinghaiensis

NAN Yan-bin, WANG Ke-xin, PAN Xue-neng, HE Qi-yue, LIU Zhan-ling, ZHOU Yuan-tao

School of Agriculture and Animal Husbandry, Qinghai University, Xining, Qinghai Province 810016, China

Received:2023-07-10 Revised:2023-10-17 Online:2023-12-15 Published:2024-01-03

摘要/Abstract

摘要： 为明确青海草原毛虫(Gynaephora qinghaiensis)幼虫感受器的类型及气味结合蛋白GqinOBPs基因在嗅觉识别过程中的功能。本研究通过扫描电镜观察青海草原毛虫6龄幼虫的感受器种类和形状，并通过实时荧光定量检测技术测定GqinOBPs基因在卵、幼虫和蛹中的表达情况。结果显示，青海草原毛虫幼虫触角上分布有7种感器，分别是腔锥形感器(SCo)、4种栓锥形感器(SSTⅠ-Ⅳ)和2种刺形感器(SChⅠ-Ⅱ)；头部及口器周围共分布9种感器，分别是3种锥形感器(SBⅠ-Ⅲ)，2种刺形感器(SChⅠ-Ⅱ)，3种栓锥形感器(SSTⅠ-Ⅲ)和1种毛型感器(ST)；足上分布有5种感器，分别是4种刺形感器的(SChⅠ-Ⅳ)和1种锥形感器；毒腺上只有Böhm氏鬃毛感器。基因表达谱分析显示，青海草原毛虫15个OBPs基因在卵、幼虫和蛹中均有不同程度的表达，其中GqinOBP4和GqinOBP10表达量较高。本研究旨在为青海草原毛虫化学感受机制奠定基础，同时也为寻找青海草原毛虫绿色防治的分子靶标提供理论依据。

关键词: 青海草原毛虫, 扫描电镜, 感受器, 气味结合蛋白, 表达谱分析

Abstract: To clarify the type of receptor and the function of GqinOBPs gene in the process of olfactory recognition,we observed the receptor species and shapes of 6th instar larvae of G.qinghaiensis by SEM and determined the expression of GqinOBPs gene in eggs,larvae,and pupae by quantitative real-time detection technology in this study. The results showed that there were 7 kinds of sensilla on the antennae of G. qinghaiensis larvae,1 kind of sensilla coeloconica,4 kinds of sensilla styloconica (SSTⅠ-Ⅳ) and 2 kinds of sensilla chaetica (SCh I-Ⅱ). There were 9 kinds of sensilla distributed around the head and mouthparts,3 sensilla basiconica (SBⅠ-Ⅲ),2 sensilla chaetica (SChⅠ-Ⅱ),3 sensilla styloconica (SSTⅠ-Ⅲ) and 1 sensilla trichodea (ST). There were 5 kinds of sensilla distributed on the foot,4 kinds of sensilla chaetica (SCh I-Ⅳ) and sensilla basiconica. There were only Böhm bristles on the poison gland. Gene expression profiling showed that The 15 OBPs genes were expressed in eggs,larvae and pupae,including OBP4 and OBP10. This study laid the foundation for a deeper understanding of the chemosensory mechanism in G. qinghaiensis,and provided a theoretical basis for finding the molecular targets of green prevention and control in G. qinghaiensis.

Key words: Gynaephora qinghaiensis, Scanning electron microscope, Sensilla, Odorant binding protein, Expression profiling

中图分类号:

S812

南彦斌, 王克鑫, 潘学能, 何啟月, 刘占玲, 周渊涛. 青海草原毛虫幼虫感器类型及GqinOBPs表达分析[J]. 草地学报, 2023, 31(12): 3626-3635.

NAN Yan-bin, WANG Ke-xin, PAN Xue-neng, HE Qi-yue, LIU Zhan-ling, ZHOU Yuan-tao. Analysis of Larval Sensor Structure and GqinOBPs Expression of Gynaephora qinghaiensis[J]. Acta Agrestia Sinica, 2023, 31(12): 3626-3635.

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青海草原毛虫幼虫感器类型及GqinOBPs表达分析

Analysis of Larval Sensor Structure and GqinOBPs Expression of Gynaephora qinghaiensis

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