基于单拷贝核基因的差不嘎蒿谱系地理与遗传结构研究

doi:10.11733/j.issn.1007-0435.2024.10.003

草地学报 ›› 2024, Vol. 32 ›› Issue (10): 3034-3042.DOI: 10.11733/j.issn.1007-0435.2024.10.003

• 研究论文 • 上一篇

基于单拷贝核基因的差不嘎蒿谱系地理与遗传结构研究

刘梦瑶, 王铁娟, 秀芳, 唐思如

内蒙古师范大学生命科学与技术学院, 内蒙古自治区高等学校蒙古高原生物多样性保护与可持续利用重点实验室, 内蒙古呼和浩特 010022

收稿日期:2024-01-26 修回日期:2024-03-24 发布日期:2024-11-04
通讯作者: 王铁娟,E-mail:wtj105@163.com
作者简介:刘梦瑶(1996-)，女，汉族，内蒙古鄂尔多斯人，硕士研究生，主要从事植物生态学研究，Email:1143343811@qq.com
基金资助:
内蒙古自治区自然科学基金项目(2019MS03041);内蒙古师范大学基本科研业务费专项资金(2022 JBTD010)资助

Phylogeography and Genetic Structure of Artemisia halodendron Based on Single Copy Nuclear Genes

LIU Meng-yao, WANG Tie-juan, XIU Fang, TANG Si-ru

College of Life Science and Technology, Inner Mongolia Normal University, Key Laboratory of Biodiversity Conservation and Sustainable Utilization in Mongolian Plateau for College and University of Inner Mongolia Autonomous Region, Hohhot, Inner Mongolia 010022, China

Received:2024-01-26 Revised:2024-03-24 Published:2024-11-04

摘要/Abstract

摘要： 为揭示我国东北部重要固沙植物差不嘎蒿(Artemisia halodendron)的群体遗传结构与种群历史动态，本研究利用2个直系同源单拷贝核基因对来自呼伦贝尔沙地和科尔沁沙地的8个种群154个个体进行分析。结果表明，2个核基因c7847和c9002分别检测到38和47个单倍型，单倍型多样性(H_d)分别为0.9474和0.9158，各种群均在0.8以上，反映出差不嘎蒿有着高的遗传多样性。2个基因的分子变异分析(AMOVA) 显示，差不嘎蒿96.524%和96.985%的遗传变异来自种群内，种群间和2个沙地间分化很小；种群遗传分化系数N_st均大于G_st，但差异不显著，表明差不嘎蒿没有明显的谱系地理结构。中性检验显示，2个核基因的Tajima’s D值均不显著，而Fu’s Fs值显著(P<0.05)，结合歧点分布分析，该种在中性模式下经历有限的种群扩张，其中分布区北部的呼伦贝尔沙地的新巴尔虎左旗(DQ)和海拉尔(HLR)种群最为明显。

关键词: 差不嘎蒿, 单拷贝核基因, 遗传结构, 谱系地理

Abstract: To reveal the population genetic structure and historical dynamics of Artemisia halodendron,an important sand-fixing plant in northeast China,phylogeographic analysis was conducted on 154 Artemisia halodendron individuals from eight populations in the Hulunbuir Sandy Land and Horqin Sandy Land using two co-dominant nuclear genes in this study. The results showed that 38 and 47 haplotypes were detected by the two nuclear genes c7847 and c9002,respectively. The haplotype diversity(H_d) was 0.9474 and 0.9158,respectively,and all populations were above 0.8,which indicated had a high genetic diversity of A. halodendron. Analysis of molecular variance (AMOVA) showed that 96.524% and 96.985% of genetic variation existed within the populations of A. halodendron based on c9002 and c7847,respectively,with limited differentiation between populations and between the two sandy lands. The population genetic differentiation coefficients N_st were all higher than G_st,but the difference was not significant (P>0.05),indicating no obvious phylogeographic structure in A. halodendron. The neutral test showed that Tajima’s D values of the two nuclear genes were not significantly different (P>0.05),while Fu’s Fs values had significant differences (P<0.05),and combined with the mismatch distribution analysis,A. halodendron experienced limited population expansion under the neutral mode. Specifically,the New Barag Left Banner (DQ) and Hailar (HLR) populations coming from the Hulunbuir Sandy Land in the northern part of the distribution area,were most pronounced.

Key words: Artemisia halodendron, Single-copy nuclear genes, Genetic structure, Phylogeography

中图分类号:

Q948.2

刘梦瑶, 王铁娟, 秀芳, 唐思如. 基于单拷贝核基因的差不嘎蒿谱系地理与遗传结构研究[J]. 草地学报, 2024, 32(10): 3034-3042.

LIU Meng-yao, WANG Tie-juan, XIU Fang, TANG Si-ru. Phylogeography and Genetic Structure of Artemisia halodendron Based on Single Copy Nuclear Genes[J]. Acta Agrestia Sinica, 2024, 32(10): 3034-3042.

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基于单拷贝核基因的差不嘎蒿谱系地理与遗传结构研究

Phylogeography and Genetic Structure of Artemisia halodendron Based on Single Copy Nuclear Genes

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