大狼毒主要化感物质对山地草甸土壤微生物物种多样性的影响

doi:10.11733/j.issn.1007-0435.2025.01.002

草地学报 ›› 2025, Vol. 33 ›› Issue (1): 10-20.DOI: 10.11733/j.issn.1007-0435.2025.01.002

• 研究论文 • 上一篇

大狼毒主要化感物质对山地草甸土壤微生物物种多样性的影响

段蕊¹, 罗钦¹, 肖雪¹, 牛琼梅¹, 刘洋², 杨雪¹, 陆嘉琪¹, 初晓辉¹, 单贵莲¹

1. 云南农业大学草业科学系, 云南昆明 650201;
2. 云南省林业与草原局, 云南昆明 650224

收稿日期:2024-07-15 修回日期:2024-09-13 发布日期:2025-01-22
通讯作者: 初晓辉,E-mail：478491637@qq.com；单贵莲,E-mail：shanguilian8203@126.com
作者简介:段蕊（2000-）,女,汉族,云南富源人,硕士研究生,主要从事毒害草化感作用研究,E-mail：gnahsoak@qq.com；罗钦（1998-）,男,汉族,云南宣威人,硕士研究生,主要从事毒害草化感作用研究,E-mail：2680142652@qq.com
基金资助:
云南省万人计划青年拔尖人才专项（YNWR-QNBJ-2018-031）；云南省农业联合专项（202101BD070001-068,202301BD070001-134,202301BD070001-174）；云南省水利科技项目（2023BG204001）资助

Effects of Main Allelopathic Substances of Euphorbia jolkinii on Species Diversity of Soil Microorganisms in Montane Meadow

DUAN Rui¹, LUO Qin¹, XIAO Xue¹, NIU Qiong-mei¹, LIU Yang², YANG Xue¹, LU Jia-qi¹, CHU Xiao-hui¹, SHAN Gui-lian¹

1. Department of Grassland Science, Yunnan Agricultural University, Kunming, Yunnan Province 650201, China;
2. Forestry and Grassland Bureau of Yunnan Province, Kunming, Yunnan Province 650224, China

Received:2024-07-15 Revised:2024-09-13 Published:2025-01-22

摘要/Abstract

摘要： 为探讨大狼毒（Euphorbia jolkinii）化感物质在其种群入侵过程中的调节作用,本试验采用人为控制添加化感物质溶液、室内培养土壤样品和宏基因组测序技术,测定分析添加4-硝基苯酚和2-苯乙胺溶液对山地草甸土壤微生物的影响。结果表明：2种化感物质的添加均会显著增加土壤微生物种水平的物种数目；4-硝基苯酚添加对土壤细菌和真菌的物种组成、丰富度和多样性均无显著影响；2-苯乙胺添加会改变土壤细菌酸杆菌门（Acidobacteria）、变形菌门（Proteobacteria）、亚硝化杆菌属（Candidatus_Nitrosotalea）和慢生根瘤菌属（Bradyrhizobium）的相对丰度,显著增加土壤细菌物种多样性和均匀度,以及真菌丰富度（P<0.05）。综上,大狼毒2种主要化感物质的添加增加了山地草甸土壤微生物物种数目,促进了土壤微生物的生长繁殖,但不同物质对土壤微生物多样性的影响存在差异,与4-硝基苯酚相比,2-苯乙胺的影响程度更高。

关键词: 大狼毒, 化感物质添加, 土壤微生物, 优势菌相对丰度, 物种多样性

Abstract: To investigate the regulatory role of Euphorbia jolkinii allelopathic substances in the invasion process of its population. In this study, we measured and analyzed the effects of addition of 4-Nitrophenol and 2-Phenylethylamine on soil microorganisms in the montane meadow by using artificially controlled addition of allelochemical solutions, indoor culture of soil samples, and macro-genome sequencing technology. The results showed that the addition of 4-Nitrophenol had no significant effect on the species composition, abundance and diversity of soil bacteria and fungi, while the addition of 2-Phenylethylamine altered the soil bacterial Acidobacteria, Proteobacteria,Candidatus_Nitrosotalea and Bradyrhizobium, and significantly increased soil bacterial species diversity and evenness, as well as fungal richness （P<0.05）. In conclusion, the addition of Euphorbia jolkinii 2 major allelochemical solutions increased the number of soil microbial species and promoted the growth and reproduction of soil microorganisms in the montane meadow, but there were differences in the effects of the different substances on the diversity of soil microorganisms, and the extent of the effect of 2-Phenylethylamine was even higher than that of 4-Nitrophenol.

Key words: Euphorbia jolkinii, Allelopathic substances addition, Soil microorganisms, Relative abundance of dominant bacteria, Species diversity

中图分类号:

S812.6

段蕊, 罗钦, 肖雪, 牛琼梅, 刘洋, 杨雪, 陆嘉琪, 初晓辉, 单贵莲. 大狼毒主要化感物质对山地草甸土壤微生物物种多样性的影响[J]. 草地学报, 2025, 33(1): 10-20.

DUAN Rui, LUO Qin, XIAO Xue, NIU Qiong-mei, LIU Yang, YANG Xue, LU Jia-qi, CHU Xiao-hui, SHAN Gui-lian. Effects of Main Allelopathic Substances of Euphorbia jolkinii on Species Diversity of Soil Microorganisms in Montane Meadow[J]. Acta Agrestia Sinica, 2025, 33(1): 10-20.

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大狼毒主要化感物质对山地草甸土壤微生物物种多样性的影响

Effects of Main Allelopathic Substances of Euphorbia jolkinii on Species Diversity of Soil Microorganisms in Montane Meadow

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