草地学报

›› 2002, Vol. 10 ›› Issue (1): 24-27.DOI: 10.11733/j.issn.1007-0435.2002.01.004

• 研究论文 • 上一篇    下一篇

苏丹草与拟高粱远缘杂交初报

钟小仙1, 顾洪如1, 丁成龙1, 白淑娟1, 向阳海2, 刘朝晖2   

  1. 1. 江苏省农业科学院土壤肥料研究所, 南京, 210014;
    2. 江苏省农业科学院遗传生理研究所, 南京, 210014
  • 收稿日期:2001-09-06 修回日期:2001-11-15 出版日期:2002-02-15 发布日期:2002-02-15
  • 作者简介:钟小仙(1968- ),女,浙江余姚人,在读博士,助理研究员,主要从事牧草种质资源收集及分子示记辅助育种研究
  • 基金资助:
    江苏省自然科学基金项目(BK2001176)

Study on Sorghum sudanese × S.propinguum interspecies hybridization

ZHONG Xiao-xian1, GU Hong-ru1, DING Cheng-long1, BAI Su-juan1, XIANG Yang-hai2, LIU Zhao-hui 2   

  1. 1. Institute of Soil and Fertilizer Institute, Jiangsu Academy of Agricultural Science, Nanjing 210014, China;
    2. Institute of Agrobiological Genetics and Physiology, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China
  • Received:2001-09-06 Revised:2001-11-15 Online:2002-02-15 Published:2002-02-15

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摘要： 通过苏丹草与拟高梁杂交,获得了远缘杂种F1代,远缘杂交成功率1%左右,F1种子的发芽率约20%.F1花粉母细胞减数分裂中期染色体镜检为10个二价体,染色体配对正常.采用RAPD分子标记技术对F1真假杂交种进行快速鉴定,提高了育种效率.杂种F1代大田表现对叶斑病轻感,F2代田间表现明显的抗/感分离,为苏丹草抗病育种提供了可能.

关键词: 苏丹草, 远缘杂交, 抗叶斑病育种

Abstract: Interspecies hybrids(F1)were obtained by distant crossing between S.sudangrass (Piper) Stapf.and S.propinguum (Kunth) Hifehe. The successful probability of hybridization is about 1% and germination of seed is 20% or so. It was observed ten bivalents chromosomes according to a microspore mother cell of F1 at metaphase I during meiosis. The chromosome comparison of F1 is normal. It will be increased the efficiency of breeding by using PAPD technique, which is applied to identified true or false of F1. Interspecies F1 plant is low infected leaf spot disease and resistant plant presented in F2. It was supplied a probability for leaf spot resistant breeding.

Key words: Sudan grass, Distant crossing, Leaf spot resistant breeding

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