草地学报

›› 2002, Vol. 10 ›› Issue (3): 221-226.DOI: 10.11733/j.issn.1007-0435.2002.03.014

• 研究论文 • 上一篇    下一篇

顿河红豆草凝集素cDNA克隆与序列分析

赵素容1, 陈大明2, 陈默君1, 杨茁萌3   

  1. 1. 中国农业大学草地科学研究所, 北京, 100094;
    2. 浙江大学园艺系, 杭州, 310029;
    3. 新疆农业大学畜牧学院草原系, 乌鲁木齐, 830052
  • 收稿日期:2002-01-10 修回日期:2002-05-30 出版日期:2002-08-15 发布日期:2002-08-15
  • 作者简介:赵素容,(1975- ),女,中国农业大学草地科学研究所硕士生,主要从事牧草与草坪的研究
  • 基金资助:
    国家自然科学基金项目(C02030102)

The Cloning and Sequencing of a Lectin Gene from Onobrychis tanaitica

ZHAO Su-rong1, CHEN Da-ming2, CHEN Mo-jun3   

  1. 1. Institute of Grassland Science, China Agricultural University, Beijing 100094, China;
    2. Department of Horticulture, Zhejiang Universitity, Hangzhou 310029, China;
    3. Department of Grassland, Xinjing Agricultural University, Urumq 830052, China
  • Received:2002-01-10 Revised:2002-05-30 Online:2002-08-15 Published:2002-08-15

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摘要： 利用RT-PCR技术从顿河红豆草含浓缩单宁的愈伤组织总RNA中分离克隆了凝集素cDNA.该cNDA包含完整的编码区和3’端非编码区,所编码的氨基酸序列与其它豆科植物凝集素具有较高的同源性。

关键词: 顿河红豆草, 凝集素, cDNA克隆, 序列分析

Abstract: A cDNA fragment was isolated and cloned from the total RNA of calli of Onobrychis tanaitica containing condensed tannin using RT-PCR technique.The cDNA is 919 bp in length and includes an open reading frame encoding peptide of 251 amino acid residues and 163 bp of 3’ uncoding region.The deduced amino acid sequence shows high homology to those of other legume leactins.

Key words: Onobrychis tanaitica Spreng., Lectin, cDNA cloning, Sequence analysis

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