草地学报

›› 2005, Vol. 13 ›› Issue (2): 126-129.DOI: 10.11733/j.issn.1007-0435.2005.02.009

• 研究论文 • 上一篇    下一篇

柠条锦鸡儿DNA提取及AFLP反应体系的建立

王赞1,2, 高洪文2, 韩建国1   

  1. 1. 中国农业大学草地所, 北京, 100094;
    2. 中国农业科学院畜牧所, 北京, 100094
  • 收稿日期:2005-03-29 修回日期:2005-05-23 出版日期:2005-05-15 发布日期:2005-05-15
  • 通讯作者: 高洪文,E-mail:gaohongwen@263.net;王赞,E-mail:wangzan99@sohu.com
  • 作者简介:王赞(1974- ),男,内蒙古磴口人,博士研究生,主要从事牧草及草坪草种质资源研究,E-mail:w angzan99@sohu.com
  • 基金资助:
    国家“863”项目(2002AA241091)

DNA Extraction Method and Profile of AFLP Fingerprint of Caragana korshinskii

WANG Zan1,2, GAO Hong-wen2, HAN Jian-guo1   

  1. 1. Institute of Grassland Science, China Agricultural University, Beijing 100094, China;
    2. Institute of Animal Science, Chinese Academy of Agricultural Science, Beijing 100094, China
  • Received:2005-03-29 Revised:2005-05-23 Online:2005-05-15 Published:2005-05-15

PDF

摘要： 以柠条锦鸡儿(Caragana korshinskii)种子为材料,利用改进的酚一氯仿法,提取到高质量的DNA,建立柠条锦鸡儿AFLP银染反应体系。结果表明:首次在柠条锦鸡儿DNA提取、酶切连接、预扩增和选择性扩增等实验过程取得成功;得到清晰的柠条锦鸡儿AFLP指纹图谱;为建立柠条锦鸡儿基因库、品种选育以及亲缘关系的研究提供参考。

关键词: 柠条锦鸡儿, DNA提取, AFLP

Abstract: The extraction of DNA with phenol-cholorofore method from Caragana korshinskii seed and AFLP fingerprint profile of C. korshinskii were studied. The high resolution AFLP fingerprints were clear,showing the success in the DNA extraction, restriction-ligase reaction,pre-amplification and silver staining techniques.The results could supply references to the gene pool establishment,selection and breeding of cultivars and genetic relationships in C. korshinskii.

Key words: Caragana korshinskii, DNA extraction, AFLP

中图分类号: