草地学报

›› 2007, Vol. 15 ›› Issue (4): 348-351.DOI: 10.11733/j.issn.1007-0435.2007.04.010

• 研究论文 • 上一篇    下一篇

长穗偃麦草与中间偃麦草杂种成熟胚离体培养与再生体系的建立

鲍芫, 米福贵   

  1. 内蒙古农业大学生态环境学院, 呼和浩特, 010018
  • 收稿日期:2006-12-25 修回日期:2007-05-28 出版日期:2007-08-15 发布日期:2007-08-15
  • 通讯作者: 米福贵,E-mail:mfgui@yahoo.com.cn
  • 作者简介:鲍芫(1980- ),女,蒙古族,内蒙古呼伦贝尔人,硕士研究生,研究方向为饲料遗传育种、栽培与加工,E-mail:baoyuan1980@163.com
  • 基金资助:
    国家转基因植物与产业化研究重大专项项目(J2002-B-008)

A Study on Induction of Emdryogenic Callus and Plant Regeneration from Mature Embryos of Elytrigia hybrid(E. elongata×E. intermedia)

BAO Yuan, MI Fu-gui   

  1. College of Ecology and Environment Science, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia Autonomous Region 010018, China
  • Received:2006-12-25 Revised:2007-05-28 Online:2007-08-15 Published:2007-08-15

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摘要： 以长穗偃麦草(Elytrigia elongata)和中间偃麦草杂交种(Elytrigia hybrid;E. elongata×E. intermedia)的成熟胚为外植体,进行组培再生体系的研究。结果表明:诱导愈伤组织的适宜培养基为MS+CH(500 mg/L)+2,4-D(1.0 mg/L)+6-BA(0.2 mg/L),其诱导率为73%,适宜分化培养基为MS+6-BA(4.0 mg/L)+NAA(0.5 mg/L),分化率最高,为37.8%。本研究建立了一套有效的以成熟胚为外植体偃麦草杂种组织培养再生体系。

关键词: 长穗偃麦草, 中间偃麦草, 杂交种, 成熟胚, 愈伤组织, 再生植株

Abstract: The regeneration from mature embryos was studied using the mature embryos of Elytrigia hybrid(E. elongata×E. intermedia) as explants.Results indicate that the frequency of callus induction reached 73% in the callus induction medium supplemented with 1.0 mg/L 2,4-D and 0.2 mg/L 6-BA.During subcultures,rapid growth of callus and embryogenesis were maintained in the callus subculture medium plus 1.0 mg/L 2,4-D and 0.5 mg/L 6-BA.After callus subcultures,the rate of shoot regeneration from callus were 37.8% in the differentiation medium supplemented with 4.0 mg/L6-BA and 0.5mg/L NAA.An effective system of mature embryos regeneration for Elytrigia hybrid(E. elongata×E. intermedia) was developed.

Key words: Elytrigia elongate(Host) Nevski, E.intermedia(Host) Nevski, E.hybrid, Mature embryo, Embryogenic callus, Plant regeneration

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