甘肃省苜蓿种质资源遗传多样性RAPD分析

doi:10.11733/j.issn.1007-0435.2008.02.006

›› 2008, Vol. 16 ›› Issue (2): 129-134.DOI: 10.11733/j.issn.1007-0435.2008.02.006

甘肃省苜蓿种质资源遗传多样性RAPD分析

杨晓莉, 陈丽, 班霆, 韩鹏, 王晓娟

兰州大学草地农业科技学院, 甘肃省草原生态研究所, 甘肃, 兰州, 730020

收稿日期:2007-05-28 修回日期:2007-09-24 出版日期:2008-04-15 发布日期:2008-04-15
通讯作者: 王晓娟,E-mail:xiaojuanwang@lzu.edu.cn
作者简介:杨晓莉(1980- ),女,甘肃武威人,硕士研究生,研究方向为牧草遗传育种,E-mail:yangx105@lzu.cn
基金资助:
国家重点研究发展计划项目(2007CB108904);甘肃省自然科学基金项目(3ZS051-A25-066);兰州大学引进人才专项基金项目(582402)

RAPD Analysis of Genetic Diversity of Alfalfa Germplasms in Gansu Province

YANG Xiao-li, CHEN Li, BAN Ting, HAN Peng, WANG Xiao-juan

College of Pastoral Agriculture Science and Technology, Lanzhou University;Gansu GrasslandEcological Research Institute, Lanzhou, Gansu Province 730020, China

Received:2007-05-28 Revised:2007-09-24 Online:2008-04-15 Published:2008-04-15

摘要/Abstract

摘要： 苜蓿(Medicago sativa L.)是世界上重要的豆科牧草,了解栽培品种的遗传关系和遗传距离对于育种工作具有重要意义。本研究利用RAPD标记技术对甘肃省11个苜蓿栽培品种(群)进行遗传多样性分析,以期为进一步开展苜蓿新品种选育奠定基础。结果表明:10个随机引物共检测到132条谱带,其中107条为多态性带,多态性条带比率(PPB)高达81.1%;天水苜蓿和定西苜蓿之间的Roger’s遗传距离较远(0.4610),而与陇中苜蓿之间的遗传距离较近(0.1406);进一步的AMOVA分析显示苜蓿种群大部分的遗传变异发生在种群内(61.25%),有38.75%的遗传变异发生在种群间,种群分化不明显。

关键词: 苜蓿, 种质资源, 遗传多样性, RAPD

Abstract: Alfalfa(Medicago sativa L.) is a forage legume of world-wide importance and the genetic relationship and distance among cultivars are of great interest for breeding programs.In this paper,random amplified polymorphic DNA(RAPD) was used to analyze the genetic diversity of 11 alfalfa cultivars.A total of 132 discernible loci were obtained for all populations using 10 primers,and 81.1% of these loci were polymorphic,which indicated a high genetic diversity in the cultivars from Gansu Province.According to the Roger’s distance,the smallest MRD(0.1406) occurred between cultivars ‘Tianshui’ and ‘Longzhong’,while the largest(0.4610) was detected between cultivars ‘Tianshui’ and ‘Dingxi’.The result of AMOVA analysis showed that the majority(61.25%) of genetic variation was within populations and 38.75% among populations.

Key words: Medicago spp., Germplasm, Genetic diversity, RAPD

中图分类号:

S812
Q943

杨晓莉, 陈丽, 班霆, 韩鹏, 王晓娟. 甘肃省苜蓿种质资源遗传多样性RAPD分析[J]. , 2008, 16(2): 129-134.

YANG Xiao-li, CHEN Li, BAN Ting, HAN Peng, WANG Xiao-juan. RAPD Analysis of Genetic Diversity of Alfalfa Germplasms in Gansu Province[J]. , 2008, 16(2): 129-134.

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甘肃省苜蓿种质资源遗传多样性RAPD分析

RAPD Analysis of Genetic Diversity of Alfalfa Germplasms in Gansu Province

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