草地学报

›› 2010, Vol. 18 ›› Issue (1): 108-114.DOI: 10.11733/j.issn.1007-0435.2010.01.021

• 研究论文 • 上一篇    下一篇

利用RAPD标记对不同秋眠级苜蓿种质的聚类和评价

刘荣霞1,2, 于林清1,5, 张宇3, 苏东4, 云锦凤5   

  1. 1. 中国农业科学院草原研究所, 内蒙古, 呼和浩特, 010010;
    2. 甘肃省金川集团公司生活服务分公司园林公司, 甘肃, 金昌, 737100;
    3. 内蒙古农业大学林学院, 内蒙古, 呼和浩特, 010018;
    4. 内蒙古大学生命科学院, 内蒙古, 呼和浩特, 010021;
    5. 内蒙古农业大学生态环境学院, 内蒙古, 呼和浩特, 010019
  • 收稿日期:2009-04-03 修回日期:2009-12-15 出版日期:2010-02-15 发布日期:2010-02-15
  • 通讯作者: 于林清,E-mail:linqing-yu@126.com
  • 作者简介:刘荣霞(1980- ),女,甘肃金昌人,硕士,研究方向为牧草种质资源与育种,E-mail: liu-rong-xia@hotmail.com
  • 基金资助:
    国家自然科学基金(30371487);国家科技支撑项目(2008BADB3B06);863项目(2008AA10Z149);内蒙古草地生态学重点实验室课题(2007-01)

Clustering and Evaluation of Alfalfa Germplasms with Different Fall Dormancy Levels by RAPD Markers

LIU Rong-xia1,2, YU Lin-qing1,5, ZHANG Yu3, WU Dong4, YUN Jin-feng5   

  1. 1. Grassland Research Institute, Chinese Academy of Agricultural Science, Hohhot, Inner Mongolia Autonomous Region 010010, China;
    2. Landscape Branch, Living Service Group, Jin Chuan Group, Jin Chang, Gansu Province 737100, China;
    3. College of Forestry Science, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia Autonomous Region 010018, China;
    4. College of Life Science, Inner Mongolia University, Hohhot, Inner Mongolia Autonomous Region 010021, China;
    5. College of Ecology and Environmental Sciences, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia Autonomous Region 010019, China
  • Received:2009-04-03 Revised:2009-12-15 Online:2010-02-15 Published:2010-02-15

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摘要： 利用RAPD标记对25份不同秋眠等级的苜蓿材料进行分析,构建了25份苜蓿材料的DNA指纹图谱,用两种方法(特异的谱带类型和不同引物谱带类型的组合)可以有效的鉴别苜蓿单株,说明RAPD标记是鉴定苜蓿的一种有效方法。通过计算25份苜蓿材料的遗传距离,进行聚类分析,探讨它们之间的亲缘关系。结果如下:25份苜蓿材料间的遗传距离介于4.69-8.14之间,说明材料间的遗传距离较大,亲缘关系较远,遗传基础较宽;从50条RAPD引物中筛选出19条引物,总共扩增出144条带,其中134条呈多态性,占93.05%,10条为单态性带,占6.94%;遗传距离为7.39时,试验材料可以分为差异明显的4类,苜蓿材料间有较大的遗传差异,这为苜蓿引种、亲本选配,分子标记辅助选择育种提供良好的理论基础和科学依据。

关键词: RAPD, 秋眠级, 苜蓿, 聚类分析

Abstract: RAPD technology was employed to analyze the 25 alfalfa accessions with different fall dormancy levels and the RAPD fingerprinting were constructed.Two independent and effective ways,including unique band types and combination of the band types of different primers,in identification of the alfalfa individuals were proved,which indicates that RAPD is very effective to identify alfalfa germplasms.The genetic distance in tested alfalfa materials was between 4.69 and 8.14,which suggested that the genetic diversity of alfalfa was high and the genetic base was wide.Totally 144 bands were generated with 19 RAPD primers,of which 134(93.05%) were polymorphic,10(6.94%) were monomorphic.The cluster analysis based on RAPD markers revealed that the tested materials belonged to four groups by λCD=7.39.There was a wide genetic difference among the tested materials,which would provide the theoretical base for genetic improvement and selection of cross-bred parents in alfalfa.

Key words: RAPD, Fall dormancy level, Alfalfa, Clustering analysis

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