草地学报

›› 2010, Vol. 18 ›› Issue (3): 378-382.DOI: 10.11733/j.issn.1007-0435.2010.03.012

• 研究报告 • 上一篇    下一篇

中间偃麦草染色体核型及C-带带型的分析比较

黄莺1, 毛培胜1, 孟林2, 张国芳2, 毛培春2   

  1. 1. 中国农业大学草业科学系, 北京, 100193;
    2. 北京市农林科学院草业与环境研究发展中心, 北京, 100097
  • 收稿日期:2009-04-20 修回日期:2009-10-11 出版日期:2010-06-15 发布日期:2010-06-15
  • 通讯作者: 毛培胜,E-mail:cgssst@sina.com
  • 作者简介:黄莺(1983- ),女,黑龙江哈尔滨人,硕士,研究方向为牧草细胞染色体组学和耐盐植物分子标记研究,E-mail:elainehuang1225@sina.com
  • 基金资助:
    国家自然科学基金项目(30571321);北京市自然科学基金项目(6082009)资助

Analysis of the Karyotype and C-banding Pattern in Elytrigia intermedia (Host) Nevski

HUANG Ying1, MAO Pei-sheng1, MENG Lin2, ZHANG Guo-fang2, MAO Pei-chun 2   

  1. 1. Department of Grassland science, China Agricultural University, Beijing, 100193, China;
    2. Beijing Research and Development Center for Grass and Environment, Beijing Academy of Agriculture and Forestry, Beijing, 100097, China
  • Received:2009-04-20 Revised:2009-10-11 Online:2010-06-15 Published:2010-06-15

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摘要： 为比较确定偃麦草属植物种质材料间的亲缘关系和进化程度,以3份不同来源的中间偃麦草(Elytrigia in-termedia(Host)Nevski)种质材料为研究对象,分别进行染色体核型及C-带带型的分析。结果表明:3份中间偃麦草种质材料均为四倍体,其中EI015的细胞染色体核型公式为2n=4x=28=2M+10m+16sm,但不显带;EI021的染色体核型公式为2n=4x=28=4M+22m(SAT)+2sm,而带型公式为2n=4x=28=5C+2CT++2CT++4T++2T++1CS++12;EI038的染色体核型公式为2n=4x=28=4M+12m+12sm(SAT),带型公式为2n=4x=28=2S++4T++1CT++1T++20,3份种质材料染色体不对称性均属2A型。综合比较染色体核型及带型变化,EI021染色体核型特征与EI015、EI038相差明显,EI015与EI038染色体核型比较相近,但EI015与EI038在带型特征上存在明显差异,说明各种质材料间亲缘关系较远,并且染色体带型分析能够更加客观地反映染色体变化。

关键词: 中间偃麦草, 染色体, 核型分析, C-带带型分析

Abstract: The plants of genus Elytrigia Desv.are widely distributed throughout the world and represented with species of diploids,tetraploids,hexaploids,octaploids,and decaploids.The karyotype and C-banding pattern of three germplasms of Elytrigia intermedia(Host) Nevski were compared and analyzed.The results of karyotype analysis show that the karyotype formula for EI015 was 2n=4x=28=2M+10m+16sm,for EI021 2n=4x=28=4M+22m(sat)+2sm,and for EI038 2n=4x=28=4M+12m+12sm(sat),and the dissymmetry of all their karyotypes belonged to 2A type.The result of C-banding analysis show that the C-banding pattern for EI021 was 2n=28=5C+2CT++2CT++4T++2T++1CS++12;for EI038 was 2n=28=2S++4T++1CT++1T++20;but there was no C-banding for EI015.According to the results of the karyotype and C-banding pattern analysis,the karyotype of EI021 was different from the other two germplasms which were close,but the C-banding pattern was different obviously between EI015 and EI038.The kinship was not close among three germplasms based on the comparison of the C-banding analysis which could impersonally reflect the chromosome difference.

Key words: Elytrigia intermedia(Host) Nevski, Chromosome, Karyotype analysis, C-banding

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