6种能源草的丛枝菌根侵染状况初步调查

doi:10.11733/j.issn.1007-0435.2014.02.028

›› 2014, Vol. 22 ›› Issue (2): 392-397.DOI: 10.11733/j.issn.1007-0435.2014.02.028

6种能源草的丛枝菌根侵染状况初步调查

赵佳佳¹, 冯国辉², 杨富裕³, 冯海艳², 董军⁴, 盖京苹¹

1. 中国农业大学资源与环境学院, 北京 100193;
2. 中国地质大学(北京)地球科学与资源学院, 北京100083;
3. 中国农业大学动物科技学院, 北京 100193;
4. 电子科技大学中山学院, 广东中山 528402

收稿日期:2013-06-25 修回日期:2013-10-04 出版日期:2014-04-15 发布日期:2014-04-21
通讯作者: 盖京苹
作者简介:赵佳佳（1989-），女，河北石家庄人，硕士研究生，研究方向为土壤微生物，E-mail：1007880614@qq.com
基金资助:
863计划（2012AA101802）；广东省自然科学基金项目（S2011010006107）；国家自然科学基金项目（41271269）资助

A Preliminary Investigation of the Arbuscular Mycorrhizal Status in Several Energy Grass

ZHAO Jia-jia¹, FENG Guo-hui², YANG Fu-yu³, FENG Hai-yan², Dong Jun⁴, GAI Jing-ping¹

1. College of Resources and Environment, China Agricultural University, Beijing 100193, China;
2. College of Earth Sciences and Resources, China University of Geosciences, Beijing 100083, China;
3. College of Animal Science and Technology, China Agricultural University, Beijing 100193, China;
4. University of Electronic Science and Technology of China, Zhongshan, Guangdong Province 528402, China

Received:2013-06-25 Revised:2013-10-04 Online:2014-04-15 Published:2014-04-21

摘要/Abstract

摘要： 共生是植物适应环境的一种重要策略，丛枝菌根真菌作为一类古老的土壤微生物，能与陆地上大多数的维管植物形成专性共生体系，在影响植物生长发育、养分吸收和生态适应性等方面起到了关键作用。对栽培于中国农业大学涿州试验站的6种能源草（8个品种）进行菌根侵染状况和孢子群落的初步调查，结果表明：所有能源草类型均能被AM真菌侵染并形成丛枝、泡囊、菌丝等典型结构，侵染水平为4.3%~25.2%，其中虉草（Phalaris arundinacea）侵染率最高，芒草（Miscanthus sinensis）最低。每20 g根际土中有18.7~48.0个孢子，菌丝密度在1.8~4.2 m·g^-1之间。经过对根外孢子的形态学鉴定，发现与调查的能源草共生的AM真菌优势种类为Funneliformis mosseae和Claroideoglomus etunicatum。这表明丛枝菌根真菌能与本研究中的能源草建立良好的共生关系，为能源草的生态适应性研究提供了新的思路。

关键词: 能源草, 丛枝菌根, 侵染, 孢子群落

Abstract: Symbiosis is an important strategy for plants to adapt to the environment. A substantial part of the soil microbial community belongs to arbuscular mycorrhizal (AM), an ancient group of fungi belonging to the phylum Glomeromycota, which forms mutualistic association with the roots of the majority of land plants. These fungi have played a key role in plant growth and development, nutrient uptake and ecological adaptability. The mycorrhizal colonization status and spore community of eight energy grass cultivars growing in the Zhuozhou experimental station of China Agricultural University were investigated in this study. The experimental results showed that all tested energy grass cultivars formed arbuscule, vesicle and hyphae, and the colonization rate ranged from 4.3% to 25.2%. The colonization rate of Phalaris arundinacea was the highest among the cultivars, while Miscanthus sinensis was the lowest. The spore densities in the rhizosphere soil of different host plant species ranged from 18.7 to 48.0 per 20 g air-dried soil, and the hyphal densities ranged from 1.8 to 4.2 meters per g. Funneliformis mosseae and Claroideoglomus etunicatum were the dominant AM fungal species establishing symbiosis with tested energy grass cultivars by spore identification. The research indicated that arbuscular mycorrhizal fungi could establish good association with the most investigated energy grass cultivars, which offered a new way for the research of ecological adaptation of energy grass.

Key words: Energy grass, Arbuscular mycorrhiza, Colonization, Spore community

中图分类号:

S154.3

赵佳佳, 冯国辉, 杨富裕, 冯海艳, 董军, 盖京苹. 6种能源草的丛枝菌根侵染状况初步调查[J]. , 2014, 22(2): 392-397.

ZHAO Jia-jia, FENG Guo-hui, YANG Fu-yu, FENG Hai-yan, Dong Jun, GAI Jing-ping. A Preliminary Investigation of the Arbuscular Mycorrhizal Status in Several Energy Grass[J]. , 2014, 22(2): 392-397.

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6种能源草的丛枝菌根侵染状况初步调查

A Preliminary Investigation of the Arbuscular Mycorrhizal Status in Several Energy Grass

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