草地学报

草地学报 ›› 2016, Vol. 24 ›› Issue (4): 825-833.DOI: 10.11733/j.issn.1007-0435.2016.04.017

• 研究论文 • 上一篇    下一篇

持续干旱对金荞麦生长、生理生态特性的影响及抗旱性评价

赵丽丽1, 王普昶2, 陈超1, 邓蓉3, 向清华3, 陈燕萍4   

  1. 1. 贵州大学动物科学学院草业科学系, 贵州 贵阳 550025;
    2. 贵州省草业研究所, 贵州 贵阳 550006;
    3. 贵州省畜牧兽医研究所, 贵州 贵阳 550005;
    4. 贵州省油菜研究所, 贵州 贵阳 550025
  • 收稿日期:2014-11-18 修回日期:2015-05-04 出版日期:2016-08-15 发布日期:2016-11-29
  • 通讯作者: 王普昶,E-mail:wangpuchang@163.com
  • 作者简介:赵丽丽(1981-),女,内蒙古通辽人,博士,副教授,从事牧草种质资源及育种研究,E-mail:zhaolili_0508@163.com
  • 基金资助:

    国家科技支撑计划（2014BAD23B03-3）；贵州省科技厅项目（黔科合NY[2014]3048号）；贵州省科技成果重点推广计划（黔科合成字（2013）5084）；贵州省动植物育种专项（黔农育专字（2012）018）；贵州省科技厅项目（黔科合NY字[2010]3049号）资助

Influence of Long-term Persistent Drought Stress on Growth, Physiological and Ecological Characteristics of Fagopyrum dibotrys and Comprehensive Evaluation of Their Drought Resistance

ZHAO Li-li1, WANG Pu-chang2, CHEN Chao1, DENG Rong3, XIANG Qing-hua3, CHEN Yan-ping4   

  1. 1. Department of Grassland Science, College of Animal Science, Guizhou University, Guiyang, Guizhou Province 550025, China;
    2. Guizhou Prataculture Institute, Guiyang, Guizhou Province 550006, China;
    3. Guizhou Institute of Animal Husbandry and Veterinary, Guiyang, Guizhou Province 550005, China;
    4. Guizhou Rape Institute, Guiyang, Guizhou Province 550025, China
  • Received:2014-11-18 Revised:2015-05-04 Online:2016-08-15 Published:2016-11-29

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摘要：

为探讨金荞麦（Fagopyrum dibotrys）对持续干旱胁迫的适应对策和能力，揭示其抗旱机制，本试验以8个优异金荞麦种质为试验材料，采用室内盆栽模拟干旱胁迫法，研究持续干旱下金荞麦细胞膜脂过氧化、渗透调节物质、保护酶活性、光合及叶绿素荧光参数变化和植株生长情况，并利用主成分与隶属函数综合评价其抗旱性。结果表明，干旱胁迫下金荞麦的脯氨酸（Pro）、可溶性糖（SS）等渗透调节物质累积，维持细胞膨压；SOD，POD和CAT等抗氧化酶活性增加，保护细胞膜免遭伤害。SS的渗透调节能力强于Pro。SOD和CAT酶在活性氧清除反应过程中，首先发挥作用。抗旱性强的金荞麦非光化学猝灭升高，保护细胞及光合结构不受损伤；叶面积、气孔导度和蒸腾速率降低，地下生物量增加保证了较高的水分利用率。8个金荞麦材料的抗旱性顺序为：JQ6 >JQ3 >JQ2 >JQ4 >JQ1 >JQ8 >JQ5 >JQ7。

关键词: 金荞麦, 抗旱性, 生长, 生理生态指标, 综合评价

Abstract:

The objectives of the study were to explore the adaptive strategies of Fagopyrum dibotrys and the ability of tolerating drought, and to reveal drought resistance mechanisms of different materials. The seedlings of eight excellent germplasms were grown in greenhouse. The effects of drought stress on the cell injury rate, MDA (malondialdehyde) content, proline content, soluble sugar content, SOD (superoxide dismutase) activity, POD (peroxidase) activity, CAT (catalase) activity, physiological indexes and growth of F. dibotrys were investigated. The drought resistance of eight germplasm resources was comprehensively evaluated. The results showed that the accumulation of proline and soluble sugar could help to maintain their expansion pressure of cell, and that the increasing of antioxidase activities, such as SOD, POD and CAT could protect cell membranes from damage. Osmotic adjustment ability of soluble sugars was stronger than proline. SOD and CAT enzyme played a role firstly in the active oxygen scavenging reaction process. Drought-resistant plants enhanced their tolerance to drought stress by increasing the non-photochemical quenching, decreasing leaf area, stomatal conductance and transpiration rate, ensuring higher water use efficiency, and increasing belowground biomass to maintain soil moisture and nutrient use efficiency. The rank of drought tolerance of eight F. dibotrys germplasm resourses is as follows, JQ6 >JQ3 >JQ2 >JQ4 >JQ1 >JQ8 >JQ5 >JQ7.

Key words: Fagopyrum dibotrys, Drought resistance, Growth, Physiological and ecological parameters, Comprehensive evaluation

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