低温胁迫下内源性ROS对磷脂酶D活性影响的研究

doi:10.11733/j.issn.1007-0435.2017.03.017

草地学报 ›› 2017, Vol. 25 ›› Issue (3): 567-573.DOI: 10.11733/j.issn.1007-0435.2017.03.017

低温胁迫下内源性ROS对磷脂酶D活性影响的研究

何文平, 杨鹏军, 张旭强, 王新霞, 仇奕之, 林美贞, 杨宁

西北师范大学生命科学学院, 甘肃兰州 730070

收稿日期:2016-06-02 修回日期:2017-03-29 出版日期:2017-06-15 发布日期:2017-08-29
通讯作者: 杨宁
作者简介:何文平(1987-),男,贵州凯里人,苗族,硕士,主要从事植物细胞信号转导研究,E-mial:1059617267@qq.com
基金资助:
国家自然科学基金（31360061）资助

Effects of Endogenous ROS on Phospholipase D Activity under Low Temperature Stress

HE Wen-ping, YANG Peng-jun, ZHANG Xu-qiang, WANG Xin-xia, QIU Yi-zhi, LIN Mei-zhen, YANG Ning

College of Life Sciences, Northwest Normal University, Lanzhou, Gansu Province 730070, China

Received:2016-06-02 Revised:2017-03-29 Online:2017-06-15 Published:2017-08-29

摘要/Abstract

摘要：

低温（4℃，0℃和-4℃）处理高山离子芥（chorispora bungeana），研究低温胁迫引发的内源性ROS对高山离子芥叶中线粒体膜结合态磷脂酶D（Phospholipase D PLD）活性的影响，以期揭示内源性ROS对高山离子芥叶中线粒体膜结合态PLD活性的调控机制。结果表明，在4℃，0℃和-4℃处理下，高山离子芥叶中H₂O₂的含量和PLD活性均高于空白对照组。在正常条件下，用不同浓度H₂O₂处理后，PLD活性高于空白对照组。当添加NADPH氧化酶抑制剂（diphenylene iodonium DPI）处理后，在3个温度胁迫下PLD活性低于对照组，在该条件下线粒体膜结合态Ca²⁺含量高于对照组。当用DPI+CaCl₂处理后，PLD活性较添加DPI处理组高，而添加Ca²⁺的螯合剂EGTA处理后，PLD活性与对照组相比表现出降低，表明低温胁迫下高山离子芥叶中Ca²⁺参与内源性ROS对PLD活性的调控。

关键词: 高山离子芥, 低温胁迫, 活性氧, 磷脂酶D, 信号转导

Abstract:

The effects of endogenous ROS on mitochondrial membrane phospholipase D(PLD) activity were studied in chorispora bungeana leaves at low temperature (4℃,0℃ and -4℃), which aimed at revealing the mechanism of how endogenous ROS regulating mitochondrial membrane-bound PLD activity. The results indicated that the H₂O₂ content and phospholipase D activity were higher than blank control at 4℃, 0℃ and -4℃. The phospholipase D activity were higher than blank control when chorispora bungeana was treated by different concentration of H₂O₂ under normal condition. When treated by DPI(diphenylene iodonium DPI), an inhibitor of NADPH oxidase, the phospholipase D activity was lower than the control. At the same time, the content of mitochondrial membrane bound Ca²⁺ content increased than the control at 4℃, 0℃ and -4℃. When treated by DPI+CaCl₂, the phospholipase D activity was higher than the group with single DPI. And when treated by EGTA as the Chelating agents of Ca²⁺, the phospholipase D activity decreased, indicating that Ca²⁺ took part in regulation of endogenous ROS to mitochondrial membrane bound phospholipase D in chorispora bungeana leaves.

Key words: Chorispora bungeana, Low temperature stress, Reactive oxygen species, Phospholipase D, Signal transduction

中图分类号:

Q945.78

何文平, 杨鹏军, 张旭强, 王新霞, 仇奕之, 林美贞, 杨宁. 低温胁迫下内源性ROS对磷脂酶D活性影响的研究[J]. 草地学报, 2017, 25(3): 567-573.

HE Wen-ping, YANG Peng-jun, ZHANG Xu-qiang, WANG Xin-xia, QIU Yi-zhi, LIN Mei-zhen, YANG Ning. Effects of Endogenous ROS on Phospholipase D Activity under Low Temperature Stress[J]. Acta Agrestia Sinica, 2017, 25(3): 567-573.

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低温胁迫下内源性ROS对磷脂酶D活性影响的研究

Effects of Endogenous ROS on Phospholipase D Activity under Low Temperature Stress

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