不同纬度来源结缕草叶片响应低温胁迫的转录组分析

doi:10.11733/j.issn.1007-0435.2023.10.008

草地学报 ›› 2023, Vol. 31 ›› Issue (10): 2968-2984.DOI: 10.11733/j.issn.1007-0435.2023.10.008

不同纬度来源结缕草叶片响应低温胁迫的转录组分析

李双铭¹, 杨勇², 胡龙兴³, 徐庆国³

1. 湖南工商大学, 湖南长沙 410205;
2. 长沙学院, 湖南长沙 410022;
3. 湖南农业大学, 湖南长沙 410128

收稿日期:2023-03-07 修回日期:2023-04-28 出版日期:2023-10-15 发布日期:2023-11-02
通讯作者: 徐庆国,E-mail:huxu0309@hunau.edu.cn
作者简介:李双铭(1978-),男,汉族,湖南新宁人,博士研究生,主要从事运动场草坪建植与管理研究,E-mail:784165611@qq.com
基金资助:
湖南省教育厅重点项目（No.20A046）资助

Transcriptomic Analysis of Leave in Two Zosiagrass Genotypes Native from Different Latitudes in Response to Low Temperature Stress

LI Shuang-ming¹, YANG Yong², HU Long-xing³, XU Qing-guo³

1. Hunan University of Technology and Business, Changsha, Hunan Province 410205, China;
2. Changsha University, Changsha, Hunan Province 410022, China;
3. Hunan Agricultural University, Changsha, Hunan Province 410128, China

Received:2023-03-07 Revised:2023-04-28 Online:2023-10-15 Published:2023-11-02

摘要/Abstract

摘要： 为探讨不同纬度来源结缕草（Zoysia japonica）对低温的响应，以结缕草种质ZG-36（高纬度）和ZG-32（低纬度）为材料，分析了适温（30℃/25℃）、冷害（8℃/2℃）和冻害（2℃/-4℃）3种温度下叶片的转录组变化。结果表明，在常温、冷害、冻害下，不同来源结楼草差异表达基因数量存在差异，冷害胁迫下ZG-32中差异表达的基因数量显著多于ZG-36，而冻害胁迫下后者多于前者；在功能分类及代谢通路富集分析中，叶片差异表达基因在生物学过程（Biological process，BP）、细胞组分（Celluar component，CC）与分子功能（Molecular function，MF）等3大分类中均有富集，但富集的组分和程度不同；ZG-36的差异表达转录因子（Transcription factor，TF）显著多于ZG-32。冷害、冻害条件下，差异表达的胚胎发育晚期丰富蛋白（Late embriogenesis abundant protein，LEA）在ZG-36中上调表达的数量显著多于ZG-32，而ZG-32中下调表达的LEA显著多于ZG-36，表明LEA基因对结缕草适应低温起了重要作用。

关键词: 纬度, 结缕草, 低温胁迫, 转录组分析, 耐寒基因

Abstract: In order to explore the response mechanism of Zoysia japonica from different latitudes to low temperature stress,ZG-36 (high latitude) and ZG-32 (low latitude) from different latitudes were used as materials to analyze the changes of transcriptome of zoysiagrass leaves under three temperatures:optimum (30℃/25℃),chilling (8℃/2℃) and freezing (2℃/-4℃). The results showed that there were differences in the number of differentially expressed genes between the two genotypes under optimum,cold and freezing temperature. The number of differentially expressed genes in ZG-32 under cold injury stress was significantly higher than that in ZG-36,while the latter was more than the former under cold injury stress;In gene ontology (GO) functional classification and KEGG metabolic pathway enrichment analysis,differentially expressed genes in leaves were enriched in three major classifications:biological process (BP),cellular component (CC),and molecular function (MF),but the enriched components and degrees were different. The differentially expressed transcription factor (TF) analysis showed that ZG-36 was significantly higher than ZG-32. Under cold and freezing conditions. The number of up-regulated late embriogenesis abundant protein (LEA) in ZG-36 was significantly higher than that of ZG-32,while downregulated LEA in ZG-32 was significantly higher than that of ZG-36. Those results indicated that LEA may play an important role in cold adaptation in zoysiagrass under low temperature.

Key words: Latitude, Zoysiagrass, Low temperature stress, Transcriptomic analysis, Cold-tolerant genes

中图分类号:

S543

李双铭, 杨勇, 胡龙兴, 徐庆国. 不同纬度来源结缕草叶片响应低温胁迫的转录组分析[J]. 草地学报, 2023, 31(10): 2968-2984.

LI Shuang-ming, YANG Yong, HU Long-xing, XU Qing-guo. Transcriptomic Analysis of Leave in Two Zosiagrass Genotypes Native from Different Latitudes in Response to Low Temperature Stress[J]. Acta Agrestia Sinica, 2023, 31(10): 2968-2984.

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不同纬度来源结缕草叶片响应低温胁迫的转录组分析

Transcriptomic Analysis of Leave in Two Zosiagrass Genotypes Native from Different Latitudes in Response to Low Temperature Stress

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