草地学报

草地学报 ›› 2021, Vol. 29 ›› Issue (4): 805-812.DOI: 10.11733/j.issn.1007-0435.2021.04.022

• 技术研发 • 上一篇    下一篇

宁夏地区玉米镰孢茎腐病病原菌的分离与鉴定

张小杰1, 王春明2, 周天旺2, 刘浩3, 沈瑞清3, 李敏权1*, 郭成1,2*   

  1. 1.甘肃农业大学植物保护学院, 甘肃 兰州 730070;
    2.甘肃省农业科学院植物保护研究所, 甘肃 兰州 730070;
    3.宁夏农林科学院植物保护研究所, 宁夏 银川 750002
  • 收稿日期:2020-12-01 修回日期:2020-12-23 发布日期:2021-06-02
  • 通讯作者: * E-mail:lmq@gsau.edu.cn; E-mail:gsguoch@126.com
  • 作者简介:张小杰(1996-),女,甘肃文县人,硕士研究生,主要从事植物病理学研究,E-mail:zhangxj6262@126.com
  • 基金资助:
    宁夏农林科学院对外科技合作专项(DW-X-2018019);宁夏回族自治区重点研发项目(2018BBF02018-15)资助

Isolation and Identification of Pathogens of Maize Fusarium Stalk Rot in Ningxia

ZHANG Xiao-jie1, WANG Chun-ming2, ZHOU Tian-wang2, LIU Hao3, SHEN Rui-qing3, LI Min-quan1*, GUO Cheng1,2*   

  1. 1. College of Plant Protection, Gansu Agricultural University, Lanzhou, Gansu Province 730070, China;
    2. Institute of Plant Protection, Gansu Academy of Agricultural Sciences, Lanzhou, Gansu Province 730070, China;
    3 Plant Protection Institute, Ningxia Agriculture and Forestry Academy, Yinchuan, Ningxia 750002, China
  • Received:2020-12-01 Revised:2020-12-23 Published:2021-06-02
  • Contact: * E-mail:lmq@gsau.edu.cn; E-mail:gsguoch@126.com

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摘要： 为明确宁夏地区玉米镰孢茎腐病病原菌的种类及优势病原菌,本研究于2020年采集宁夏地区玉米(Zea mays L.)茎腐病样品36份,对所采集样品进行组织学分离、纯化和单孢分离,参照Nelson等分类系统进行形态学鉴定,并结合镰孢菌特异性引物EF-1α进行序列分析。本研究共获得209株镰孢菌,包括禾谷镰孢复合种(Fusarium graminearum species complex)、拟轮枝镰孢(F. verticillioides)、木贼镰孢(F. equiseti)、层出镰孢(F. proliferatum)、胶孢镰孢(F. subglutinans)、半裸镰孢(F. incarnatum)、锐顶镰孢(F.acuminatum.)、黄色镰孢(F.culmorum)和燕麦镰孢(F.avenaceum),其分离频率依次为28.2%,23.9%,20.1%,8.1%,7.2%,6.7%,2.9%,1.9%和1.0%。其中,禾谷镰孢复合种在宁夏5市均有分布,且致病性强,为宁夏地区玉米镰孢茎腐病优势致病菌。

关键词: 宁夏, 玉米茎腐病, 镰孢菌, TEF序列鉴定

Abstract: In order to clarify the species diversity and dominant species of Fusarium causing maize stalk rot in Ningxia,in 2020,36 samples of maize stalk rot in Ningxia were collected. After tissue isolation,purification,and single-spore isolation,the obtained isolates were morphologically identified following Nelson and other classification systems,and molecularly determined according to their EF-1α sequences. The results showed that a total of 209 strains of Fusarium were obtained,including Fusarium graminearum species complex, F. verticillioides, F. equiseti, F. proliferatum, F. subglutinans, F. incarnatum,F. acuminatum,F. culmorum and F.avenaceum,whose isolation frequencies were 28.2%,23.9%,20.1%,8.1%,7.2%,6.7%,2.9%,1.9% and 1.0% respectively. Among them, F. graminearum species complex was distributed in all the five cities of Ningxia,and its pathogenicity was strong,so it was the dominant pathogen of maize stalk rot in Ningxia.

Key words: Ningxia, Maize Stalk Rot, Fusarium, TEF sequence identification

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