纤维素降解菌和温度对水稻秸秆纤维降解和发酵品质的影响

doi:10.11733/j.issn.1007-0435.2024.10.032

草地学报 ›› 2024, Vol. 32 ›› Issue (10): 3305-3312.DOI: 10.11733/j.issn.1007-0435.2024.10.032

• 技术研发 • 上一篇

纤维素降解菌和温度对水稻秸秆纤维降解和发酵品质的影响

杨丹, 周雨歆, 尹含雪, 陈丹丹, 张庆

华南农业大学林学与风景园林学院, 广东木本饲料工程技术研究中心, 广东省森林植物种质创新与利用重点实验室, 广东广州 510642

收稿日期:2024-04-08 修回日期:2024-06-12 发布日期:2024-11-04
通讯作者: 张庆,E-mail:zqing1988@126.com
作者简介:杨丹(1999-)，女，汉族，江西南昌人，硕士研究生，主要从事牧草青贮加工研究，E-mail:ydycbwywtb@163.com
基金资助:
广州市科技特派员项目(2024E04J0301);国家重点研发项目(2022YFE0111000-2，2022YFD1300901)资助

Effects of Cellulose-degrading Bacteria and Temperature on Fibre Degradation and Fermentation Quality of Rice Straw

YANG Dan, ZHOU Yu-xin, YIN Han-xue, CHEN Dan-dan, ZHANG Qing

College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou, Guangdong Province 510642, China

Received:2024-04-08 Revised:2024-06-12 Published:2024-11-04

摘要/Abstract

摘要： 本研究从白蚁肠道中筛选出了一株纤维素降解菌，并探讨其在不同温度下水稻(Oryza sativa L.)秸秆青贮中的发酵效果。青贮试验设置对照组(CK)和纤维素降解菌处理组(CS)，纤维素降解菌添加量为1×10⁷cfu·g^-1，分别进行常温(28℃)和高温(37℃)青贮，30 d后取样分析青贮品质。结果表明，与对照组相比，添加纤维素降解菌在常温条件下能够显著增加乳酸菌数量和乳酸含量，显著降低pH值(P<0.05)；在高温条件下降低了氨态氮含量，pH值显著下降(P<0.05)。两种温度条件下，添加纤维素降解菌均显著降低中性洗涤纤维和酸性洗涤纤维含量(P<0.05)。综上所述，纤维素降解菌有助于降解纤维素，促进乳酸发酵，对水稻秸秆青贮饲料发酵品质具有改善作用。在37℃条件下，水稻秸秆青贮饲料纤维降解效果更佳。

关键词: 纤维素降解菌, 纤维, 水稻秸秆, 温度, 青贮

Abstract: This study isolated a cellulose-degrading bacterium from the termite gut and investigated its fermentation effects on rice straw silage at different temperatures. The silage experiment included a control group (CK) and a cellulose-degrading bacterium treatment group (CS),with the addition of cellulose-degrading bacteria at a concentration of 1×10⁷ cfu·g^-1. Fermentation was conducted at room temperature (28℃) and high temperature (37℃) for 30 days,after which samples were taken for quality analysis. The findings indicated that compared to the control,the addition of the cellulose-degrading bacterium significantly increased the quantity and content of lactic acid bacteria,markedly reduced pH values (P<0.05) under normal temperature condition,and lowered ammonia nitrogen content (P>0.05) while significantly reducing pH values (P<0.05) under high-temperature condition. In both temperature conditions,the cellulose-degrading bacterium notably decreased the levels of neutral detergent fiber and acid detergent fiber content (P<0.05). In summary,the cellulose-degrading bacterium aided in cellulose degradation,promoted lactic acid fermentation,and improved the fermentation quality of rice straw silage. Particularly,the effectiveness of fiber degradation in rice straw silage feed was enhanced under 37℃ condition.

Key words: Cellulose-degrading bacteria, Fiber, Rice straw, Temperature, Silage

中图分类号:

S816.53

杨丹, 周雨歆, 尹含雪, 陈丹丹, 张庆. 纤维素降解菌和温度对水稻秸秆纤维降解和发酵品质的影响[J]. 草地学报, 2024, 32(10): 3305-3312.

YANG Dan, ZHOU Yu-xin, YIN Han-xue, CHEN Dan-dan, ZHANG Qing. Effects of Cellulose-degrading Bacteria and Temperature on Fibre Degradation and Fermentation Quality of Rice Straw[J]. Acta Agrestia Sinica, 2024, 32(10): 3305-3312.

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纤维素降解菌和温度对水稻秸秆纤维降解和发酵品质的影响

Effects of Cellulose-degrading Bacteria and Temperature on Fibre Degradation and Fermentation Quality of Rice Straw

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