苯乳酸和温度对玉米秸秆青贮发酵品质及抗性基因的影响

doi:10.11733/j.issn.1007-0435.2024.07.030

草地学报 ›› 2024, Vol. 32 ›› Issue (7): 2290-2296.DOI: 10.11733/j.issn.1007-0435.2024.07.030

• 技术研发 • 上一篇

苯乳酸和温度对玉米秸秆青贮发酵品质及抗性基因的影响

杨丹, 尹含雪, 张庆

华南农业大学林学与风景园林学院, 广东广州 510642

收稿日期:2024-04-17 修回日期:2024-05-21 发布日期:2024-08-03
通讯作者: 张庆，E-mail:zqing1988@126.com
作者简介:杨丹(1999-)，女，汉族，硕士研究生，江西南昌人，主要从事牧草青贮加工研究，E-mail：ydycbwywtb@163.com
基金资助:
国家重点研发项目(2022YFE0111000-2，2022YFD1300901)资助

Impacts of Phenyllactic Acid and Temperature on the Fermentation Quality and Antibiotic Resistance Genes of Corn Straw Silage

YANG Dan, YIN Han-xue, ZHANG Qing

College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou, Guangdong Province 510642, China

Received:2024-04-17 Revised:2024-05-21 Published:2024-08-03

摘要/Abstract

摘要： 本研究旨在探究不同温度下添加不同浓度苯乳酸(Phenyl lactic acid，PLA)对玉米(Zea mays L.)秸秆青贮饲料发酵品质、抗生素抗性基因(Antibiotic resistance genes，ARGs)和可移动遗传元件(Mobile genetic genes，MGEs)丰度变化的影响。采用双因素(苯乳酸×温度)完全随机设计，温度设定20℃和30℃，苯乳酸设置添加组(0.2% PLA和0.4% PLA)和对照组(CK)，30 d后取样分析青贮品质。结果显示，与对照组相比，随着PLA浓度增加，两种温度下的pH值、乳酸菌数量和乙酸含量均显著增加(P＜0.05)。酵母菌数量随着PLA浓度增加而增加，而乳酸和氨态氮含量随之下降。与20℃相比，30℃条件下自然青贮的玉米秸秆饲料pH值显著增加，乳酸菌数量显著下降(P＜0.05)。本试验所检测的ARGs属于大环内脂类、磺胺类和四环素类抗性基因，添加PLA发酵30 d后，与对照组相比，这些ARGs和MGEs绝对丰度均显著下降(P＜0.05)，其中sul1，sul2，sul3，ermB，tetC与Tn916/1545丰度变化趋势相似，Pearson相关性分析发现两者之间具有极显著正相关关系(P＜0.01)。综上，两种温度条件下，添加PLA对玉米秸秆青贮发酵品质均具有改善作用，可以减少ARGs的污染。

关键词: 玉米秸秆, 抗生素抗性基因, 苯乳酸, 温度, 青贮

Abstract: This study aimed to investigate the effects of adding different concentrations of phenyl lactic acid (PLA) at various temperatures on the fermentation quality,abundance changes of antibiotic resistance genes (ARGs),and mobile genetic elements (MGEs) of corn (Zea mays L.) straw silage. A two-factor completely randomized design was employed,with PLA concentration and temperature as the factors. The experimental groups included a control group (CK) and addition groups (0.2%PLA and 0.4%PLA) at temperatures of 20℃ and 30℃. After 30 days,samples were collected for analysis of silage quality. The results revealed that the addition of PLA significantly increased the pH value and the quantity of lactic acid bacteria at both temperatures (P＜0.05). At a storage temperature of 20℃,there was a significant increase in acetic acid content (P＜0.05),while ammonia nitrogen content decreased. Moreover,the number of lactic acid bacteria and butyric acid content were significantly higher at 20℃ compared to 30℃ (P＜0.05). The detected ARGs in this experiment belonged to the macrolide,sulfonamide,and tetracycline classes of resistance genes. After 30 days of fermentation with PLA addition,the absolute abundances of these ARGs and MGEs significantly decreased (P＜0.05). Among them,sul1,sul2,sul3,ermB, tetC,and Tn916/1545 exhibited similar trends in abundance changes,and Pearson correlation analysis revealed a highly significant positive correlation between them (P＜0.01).In conclusion,the addition of PLA to corn straw silage improved the fermentation quality and reduced pollution from ARGs under both temperature conditions.

Key words: Corn straw, Antibiotic resistance genes, Phenyllactic acid, Temperature, Silage

中图分类号:

S816.5+3

杨丹, 尹含雪, 张庆. 苯乳酸和温度对玉米秸秆青贮发酵品质及抗性基因的影响[J]. 草地学报, 2024, 32(7): 2290-2296.

YANG Dan, YIN Han-xue, ZHANG Qing. Impacts of Phenyllactic Acid and Temperature on the Fermentation Quality and Antibiotic Resistance Genes of Corn Straw Silage[J]. Acta Agrestia Sinica, 2024, 32(7): 2290-2296.

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https://manu40.magtech.com.cn/Jweb_cdxb/CN/Y2024/V32/I7/2290

参考文献

[1] 王海虎,尚涛,黄永龙. 甘肃崇信县玉米秸秆青贮现状与改进措施[J]. 现代畜牧科技,2023(3):59-61
[2] 王瑨,张长庆,黄建伟,等. 平凉市玉米秸秆在牛产业发展中的应用现状及对策[J]. 畜牧兽医杂志,2024,43(2):32-34
[3] 聂倾国,聂永芳. 玉米秸秆的饲料化利用及其对饲养成本和经济效益的影响[J]. 中国饲料,2023(24):118-120
[4] 张霞. 青贮饲料中抗生素抗性基因的分布特征与传播机制[D]. 兰州:兰州大学,2023:23-46
[5] 周雨婷. 中国农田土壤中抗生素抗性基因分布及典型污染物浓度特征、对其影响与风险评估[D]. 杭州:浙江大学,2020:25-38
[6] 何良英. 典型畜禽养殖环境中抗生素耐药基因的污染特征与扩散机理研究[D]. 广州:中国科学院研究生院(广州地球化学研究所),2016:12-42
[7] ZHANG X,DING Z,USMAN S,et al. Metagenomics insights into the effects of lactic acid bacteria inoculation on the biological reduction of antibiotic resistance genes in alfalfa silage[J]. Journal of Hazardous Materials,2023,443:130329
[8] ZHANG X,USMAN S,BATURE I,et al. Occurrence and fate of antibiotic-resistance genes and their potential hosts in high-moisture alfalfa silage treated with or without formic acid bactericide[J]. Journal of Environmental Management,2023,347:119235
[9] WU Z,LUO Y,BAO J,et al. Additives affect the distribution of metabolic profile,microbial communities and antibiotic resistance genes in high-moisture sweet corn kernel silage[J]. Bioresource Technology,2020,315:123821
[10] ZHANG Q,ZOU X,WU S,et al. Effects of pyroligneous acid on diversity and dynamics of antibiotic resistance genes in alfalfa silage[J]. Microbiol Spectrum,2022,10(4):e155422
[11] 陆永祥,李平,白史且. 苯乳酸的研究进展[J]. 草学,2020(4):19-24
[12] 高羽莎,李奕星,李芬芳,等. 苯乳酸处理对香蕉果实后熟品质的影响[J]. 保鲜与加工,2024,24(2):8-14
[13] SUN H,LIAO C,CHEN L,et al. Potential for volatile fatty acid production via anaerobically-fermenting rice straw pretreated with silage effluent and phenyllactic acid[J]. Bioresource Technology，2023,369:128355
[14] LU Y,LI P,BAI S,et al. Effect of phenyllactic acid on silage fermentation and bacterial community of reed canary grass on the Qinghai Tibetan Plateau[J]. BMC Microbiolgy,2022,22(1):83
[15] WANG J P,YOO J S,LEE J H,et al. Effects of phenyllactic acid on production performance,egg quality parameters,and blood characteristics in laying hens[J]. Journal of Applied Poultry Research,2009,18(2):203-209
[16] WU Z,XU S,YUN Y,et al. Effect of 3-phenyllactic acid and 3-phenyllactic acid-producing lactic acid bacteria on the characteristics of alfalfa silage[J]. Agriculture-Basel,2020,10(1):10
[17] 任海伟,孙文丽,裴佳雯,等. 不同温度下干玉米秸秆与废弃白菜混贮品质差异及微生物多样性[J]. 应用基础与工程科学学报,2020,28(4):788-804
[18] WANG C,HE L,XING Y,et al. Fermentation quality and microbial community of alfalfa and stylo silage mixed with Moringa oleifera leaves[J]. Bioresource Technology,2019,284:240-247
[19] HE L,WANG C,XING Y,et al. Dynamics of proteolysis,protease activity and bacterial community of Neolamarckia cadamba leaves silage and the effects of formic acid and Lactobacillus farciminis[J]. Bioresource Technology,2019,294:122127
[20] 杨丹,吴硕,陈丹丹,等. 黄梁木精油活性成分测定及其对全株玉米青贮有氧稳定的影响[J]. 草地学报,2023,31(9):2860-2867
[21] 石赟赟,李鑫,钟金生. 重金属Cu影响下水体-沉积物界面ARGs迁移规律[C]//2023中国水利学术大会论文集.2023:244-256
[22] 刘楠. 集约化肉鸡饲养场抗生素抗性基因的分布及污染特征[D]. 杨凌:西北农林科技大学,2021:20-38
[23] 王红莉,刘丽娟,张德锋,等. 水产用微生物制剂源细菌的分离鉴定及耐药性分析[J]. 淡水渔业,2023,53(4):12-25
[24] ZHOU X,ZHOU J,XIN F,et al. Heterologous expression of a novel d-lactate dehydrogenase from Lactobacillus sp. ZX1 and its application for d-phenyllactic acid production[J]. International Journal of Biological Macromolecules,2018,119:1171-1178
[25] 陈丹丹,黄佩珊,张超,等. 植物乳杆菌对荔枝叶青贮品质及体外降解特性的影响[J]. 草地学报,2023,31(7):2232-2240
[26] 何家俊,赵国强,李莉,等. 纤维素酶对玉米秸秆与酒糟混合青贮营养成分、发酵品质、瘤胃体外降解率及微生物多样性的影响[J]. 动物营养学报,2023,35(5):3190-3200
[27] 李梦玉. 温度对玉米秸秆与白菜混贮品质的影响及细菌多样性研究[D]. 兰州:兰州理工大学,2017:22-36
[28] ZHANG J,LU T,CHAI Y,et al. Which animal type contributes the most to the emission of antibiotic resistance genes in large-scale swine farms in China?[J]. Science of the Total Environment,2019,658:152-159
[29] 许冬梅. 不同气候区及乳酸菌影响玉米青贮发酵的微生物组与代谢组学机制研究[D]. 兰州:兰州大学,2021:32-38
[30] 阮敏,吴希锴,杨朝晖,等. 温度对污泥堆肥过程中抗生素抗性基因及其潜在宿主的影响[J]. 环境工程,2023,41(5):75-83
[31] 刘逸超,包健,司强,等. 牧草青贮有氧稳定性的研究进展[J]. 中国饲料,2023(23):1-8
[32] XIE W Y,SHEN Q,ZHAO F J. Antibiotics and antibiotic resistance from animal manures to soil:a review[J]. European Journal of Soil Science,2018,69(1):181-195
[33] JACOBSEN L,WILCKS A,HAMMER K,et al. Horizontal transfer of tet(M) and erm(B) resistance plasmids from food strains of Lactobacillus plantarum to Enterococcus faecalis JH2-2 in the gastrointestinal tract of gnotobiotic rats[J]. FEMS Microbiology Ecology,2007,59(1):158-166
[34] BENGTSSON-PALME J,KRISTIANSSON E,LARSSON D G J. Environmental factors influencing the development and spread of antibiotic resistance[J]. FEMS Microbiology Reviews,2018,42(1):68-80
[35] 宋磊,何云天,柳彦俊,等. 铝形态对铝系混凝剂去除抗性基因的影响[J]. 环境科学学报,2024,40(12):4249-4262

苯乳酸和温度对玉米秸秆青贮发酵品质及抗性基因的影响

Impacts of Phenyllactic Acid and Temperature on the Fermentation Quality and Antibiotic Resistance Genes of Corn Straw Silage

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