青海湖沼泽湿地cbbL固碳微生物对模拟增温的响应

doi:10.11733/j.issn.1007-0435.2025.03.007

草地学报 ›› 2025, Vol. 33 ›› Issue (3): 728-738.DOI: 10.11733/j.issn.1007-0435.2025.03.007

• 研究论文 • 上一篇

青海湖沼泽湿地cbbL固碳微生物对模拟增温的响应

章妮^1,2,3, 陈克龙^1,2,3, 杨紫唯^1,2,3, 杨艳丽^1,2,3, 李莹^1,2,3, 王明宇^1,2,3

1. 青海师范大学地理科学学院青海省自然地理与环境过程重点实验室, 青海西宁 810008;
2. 青海师范大学青藏高原地表过程与生态保护教育部重点实验室, 青海西宁 810008;
3. 国家林业和草原局青海湖湿地生态系统国家定位观测研究站, 青海海北 812200

收稿日期:2024-07-10 修回日期:2024-09-10 发布日期:2025-04-07
通讯作者: 陈克龙,E-mail:ckl7813@163.com
作者简介:章妮（1997-）,女,汉族,湖北荆州人,博士研究生,主要从事湿地生态学研究,E-mail：1581146264@qq.com
基金资助:
第二次青藏高原综合科学考察项目（2019QZKK0405）;青海省重点研发与转化计划（2022-QY-204）;青海省科技计划（2023-ZJ-905T）资助

Response of cbbL Carbon Sequestration Microorganisms to Simulated Warming in the Marsh Wetland of Qinghai Lake

ZHANG Ni^1,2,3, CHEN Ke-long^1,2,3, YANG Zi-wei^1,2,3, YANG Yan-li^1,2,3, LI Ying^1,2,3, WANG Ming-yu^1,2,3

1. Qinghai Province Key Laboratory of Physical Geography and Environmental Process, College of Geographical Science, Qinghai Normal University, Xining, Qinghai Province 810008, China;
2. Key Laboratory of Tibetan Plateau Land Surface Processes and Ecological Conservation (Ministry of Education), Qinghai Normal University, Xining, Qinghai Province 810008, China;
3. National Positioning Observation and Research Station of Qinghai Lake Wetland Ecosystem in Qinghai, National Forestry and Grassland Administration, Haibei, Qinghai Province 812200, China

Received:2024-07-10 Revised:2024-09-10 Published:2025-04-07

摘要/Abstract

摘要： 高寒湿地碳循环过程受温度升高的深刻影响,然而高寒沼泽湿地固碳微生物群落对温度升高的响应的研究尚不完善。本研究通过开顶箱进行增温处理,利用高通量测序技术,探讨了青海湖沼泽湿地cbbL固碳微生物群落特征对温度升高的响应模式。结果显示：变形菌门是青海湖沼泽湿地cbbL固碳微生物的优势菌群,固碳微生物群落结构对增温响应明显。LEfSe分析显示,纲水平到属水平的酸硫杆菌的相对丰度均显著降低,而科水平和属水平的硫盐嗜生菌的相对丰度显著增加,可作为增温处理下的重要指示性菌群。冗余分析显示土壤pH是最重要的影响因子。此外,固碳微生物的群落多样性与全碳含量呈现了高度相关性,增温显著降低了土壤湿度及碳氮含量。本研究为未来研究气候变化影响下高寒沼泽生态系统的变化提供了坚实的基础,同时对阐明碳循环的机制具有重要意义。

关键词: 模拟增温, 固碳微生物, 气候变化, 碳固定, 高寒湿地

Abstract: The carbon cycle process of alpine wetland is deeply affected by temperature increase due to global warming, but the response of carbon sequestration microbial community to temperature increase in alpine wetland is limited. In this study, the response model of cbbL carbon sequestration microbial community to simulated temperature increase in Qinghai Lake wetland was investigated by using high-throughput sequencing technology. The results showed that Proteobacteria was the dominant group of cbbL carbon sequestration microorganisms in Qinghai Lake wetland, and the community structure of carbon sequestration microorganisms had obvious response to temperature increase. LEfSe analysis showed that the relative abundance of Acidithiobacillus decreased significantly from class level to genus level, while the relative abundance of Thiohalophilus at family level and genus level increased significantly, which could be used as an important indicator of bacteria group under warming treatment. Redundancy analysis showed that soil pH was the most important factor. In addition, the community diversity of carbon sequestration microorganisms was highly correlated with total carbon content, and increasing temperature significantly reduced soil moisture and carbon and nitrogen contents. The results of this study provide a solid foundation for future research on the changes of alpine swamp ecosystem under the influence of climate change, and have important significance for elucidating the mechanism of carbon cycle.

Key words: Simulated warming, Carbon sequestration microorganism, Climate change, Carbon fixation, Alpine wetlands

中图分类号:

X172

章妮, 陈克龙, 杨紫唯, 杨艳丽, 李莹, 王明宇. 青海湖沼泽湿地cbbL固碳微生物对模拟增温的响应[J]. 草地学报, 2025, 33(3): 728-738.

ZHANG Ni, CHEN Ke-long, YANG Zi-wei, YANG Yan-li, LI Ying, WANG Ming-yu. Response of cbbL Carbon Sequestration Microorganisms to Simulated Warming in the Marsh Wetland of Qinghai Lake[J]. Acta Agrestia Sinica, 2025, 33(3): 728-738.

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青海湖沼泽湿地cbbL固碳微生物对模拟增温的响应

Response of cbbL Carbon Sequestration Microorganisms to Simulated Warming in the Marsh Wetland of Qinghai Lake

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