草地学报

草地学报 ›› 2026, Vol. 34 ›› Issue (1): 87-97.DOI: 10.11733/j.issn.1007-0435.2026.01.009

• 研究论文 • 上一篇    

补播模式对喀斯特地区草地土壤养分和酶化学计量特征的影响

冯阳1, 高小叶1,2, 杨学东3, 王春梅4, 罗彬3, 张涛5   

  1. 1. 贵州民族大学生态环境工程学院, 贵州 贵阳 550025;
    2. 天津大学地球系统科学学院, 天津 300072;
    3. 贵州省草地技术试验推广站, 贵州 贵阳 550025;
    4. 贵州大学动物科学学院, 贵州 贵阳 550025;
    5. 贵州大学乡村振兴研究院, 贵州 贵阳 550025
  • 收稿日期:2025-01-14 修回日期:2025-04-11 发布日期:2025-12-24
  • 通讯作者: 高小叶,E-mail:gaoxiaoye1220@163.com
  • 作者简介:冯阳（2000-），男，汉族，贵州遵义人，硕士研究生，主要从事农业资源与环境研究，E-mail:fengyang3920@163.com；
  • 基金资助:
    国家自然科学基金（32360807）；贵州省科技计划项目（ZK2022一般146）；中央林业草原科技推广项目（贵（2024）TG23号）资助

Effects of Reseeding Modes on Soil Nutrient and Enzymatic Stoichiometry Characteristics of Karst Grassland

FENG Yang1, GAO Xiao-ye1,2, YANG Xue-dong3, WANG Chun-mei4, LUO Bin3, ZHANG Tao5   

  1. 1. College of Ecological and Environmental Engineering, Guizhou Minzu University, Guiyang, Guizhou Province 550025, China;
    2. School of Earth System Science, Tianjin University, Tianjin 300072, China;
    3. Guizhou Grassland Technology Experiment and Promotion Station, Guiyang, Guizhou Province 550025, China;
    4. College of Animal Science, Guizhou University, Guiyang, Guizhou Province 550025, China;
    5. Institute of Rural Revitalization, Guizhou University, Guiyang, Guizhou Province 550025, China
  • Received:2025-01-14 Revised:2025-04-11 Published:2025-12-24

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摘要： 为探究补播模式对退化草地土壤养分和酶化学计量特征的影响，以贵州独山退化草地为研究对象，以退化草地（CK）为对照，设置燕麦（Avena sativa L.）+黑麦草（Lolium perenne L.）（AL）和燕麦+黑麦草+三叶草（Trifolium L.）（ALT）补播2个处理。结果表明：AL和ALT显著增加0~10 cm土层有机碳、全氮和速效磷含量，增幅分别为42.66%和57.29%、75.54%和116.30%、90.78%和155.59%。ALT处理下0~10 cm土层C/N显著降低，N/P提高，缓解了土壤氮缺乏；β-1，4-葡萄糖苷酶和β-1，4-N-乙酰葡糖氨糖苷酶活性分别显著增加182.21%和186.85%，且增幅高于AL。ALT显著增加0~10 cm土层酶的矢量长度，加剧了微生物碳限制。相关分析表明，胞外酶活性主要受全氮和有机碳的共同调控。综上，该区域选择ALT补播模式可有效提高退化草地土壤有机碳和有效养分，缓解氮缺乏，提高土壤碳、氮循环相关酶活性，但是增强了表层土壤微生物的碳限制。

关键词: 豆科, 退化草地, 可利用养分, 胞外酶活性

Abstract: To investigate the effects of reseeding patterns on soil nutrients and enzyme stoichiometry of degraded grasslands， a study was conducted in the Dushan of Guizhou， China， using a degraded grassland （CK） as the control. Two reseeding treatments were established： oat （Avena sativa L.） + ryegrass （Lolium perenne L.） （AL） and oat + ryegrass + clover （Trifolium L.） （ALT）. The results showed that both AL and ALT significantly increased the contents of organic carbon， total nitrogen， and available phosphorus in the 0~10 cm soil layer， with increasing by 42.66% and 57.29%， 75.54% and 116.30%， and 90.78% and 155.59%， respectively. Under the ALT treatment， the C/N ratio in the 0-10 cm soil layer significantly decreased， while the N/P ratio increased， alleviating soil nitrogen deficiency. The activities of β-1，4-glucosidase and β-1，4-N-acetylglucosaminidase significantly increased by 182.21% and 186.85%， respectively， with a higher increase than that in the AL treatment. ALT significantly increased the enzyme vector length in the 0~10 cm soil layer， intensifying microbial carbon limitation. Correlation analysis indicated that extracellular enzyme activities were mainly regulated by the combined effects of total nitrogen and organic carbon. In conclusion， the ALT reseeding pattern could effectively improve soil organic carbon and available nutrients， alleviate nitrogen deficiency， and enhance soil enzyme activities related to carbon and nitrogen cycling in degraded grasslands in this region. However， it also strengthened the carbon limitation of surface soil microorganisms.

Key words: Legume, Degraded grasslands, Available nutrients, Extracellular enzyme activities

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