沙木蓼MYB-related基因家族的鉴定及其响应干旱胁迫的表达模式研究

doi:10.11733/j.issn.1007-0435.2026.06.005

草地学报 ›› 2026, Vol. 34 ›› Issue (6): 2012-2021.DOI: 10.11733/j.issn.1007-0435.2026.06.005

• 研究论文 • 上一篇

沙木蓼MYB-related基因家族的鉴定及其响应干旱胁迫的表达模式研究

何彩¹, 刘源², 晋敏¹, 吴媛¹, 王素桢¹, 胡芳¹

1. 武威市林业科学研究院, 甘肃武威 733000;
2. 甘肃农业大学林学院, 甘肃兰州 730000

收稿日期:2025-08-28 修回日期:2025-10-16 发布日期:2026-06-02
通讯作者: 何彩,E-mail:hcyldfcl@163.com
作者简介:何彩(1985-),女,汉族,甘肃武威人,博士,正高级工程师,主要从事荒漠化防治研究,E-mail:hcyldfcl@163.com
基金资助:
甘肃省科技计划项目（自然科学基金）（24JRRH007，25JRRH004）；武威市科技创新人才计划项目（WW25A01RYFL002）资助

Identification of MYB-related Gene Family in Atraphaxis bracteata and Study on its Expression Pattern in Response to Drought Stress

HE Cai¹, LIU Yuan², JIN Min¹, WU Yuan¹, WANG Su-zhen¹, HU Fang¹

1. Wuwei Academy of Forestry, Wuwei, Gansu Province 733000, China;
2. College of Forestry, Gansu Agricultural University, Lanzhou, Gansu Province 730000, China

Received:2025-08-28 Revised:2025-10-16 Published:2026-06-02

摘要/Abstract

摘要： MYB-related基因家族在植物生长发育及逆境胁迫中起关键作用。本研究基于沙木蓼（Atraphaxis bracteata）全长转录组数据，采用生物信息学方法鉴定了其MYB-related基因家族，分析了进化关系、基因结构、理化性质，并采用qRT-PCR分析了其在干旱胁迫下各组织的时空表达模式。结果显示，共鉴定出55个成员，分为5个亚组；编码蛋白多呈亲水性，主要定位于细胞核，Motif 2为特征基序，Motif 4保守性最高，蛋白质二、三级结构以无规则卷曲为主。表达分析表明，干旱胁迫24 h后基因表达量均高于6 h和0 h，多数基因在叶中表达较高，这些基因在根茎中对重度干旱响应更强，而在叶中则对轻度干旱更为敏感。综上，沙木蓼MYB-related基因家族在干旱胁迫中具有组织特异性表达模式，为今后功能研究与抗逆育种提供了理论依据。

关键词: 沙木蓼, MYB-related, 干旱胁迫, 表达模式

Abstract: MYB-related gene family plays important roles in plant growth， development， and stress response. This study analyzed the number， evolutionary relationships， gene structure， and physicochemical properties of MYB-related family genes in Atraphaxis bracteata by bioinformatics methods based on the full-length transcriptome data of A. bracteate. The qRT-PCR was used to analyze the temporal and spatial expression patterns of these genes in various tissues of A. bracteata under drought stress， and to analyze their response to drought stress. The results showed that a total of 55 MYB-related family members of A. bracteata were identified and divided into 5 subgroups. The encoded proteins were mostly hydrophilic and mainly located in the nucleus. Motif 2 was the characteristic motif， while Motif 4 had the highest conservation. The secondary and tertiary structures of their proteins were mainly irregularly coiled. Expression analysis showed that after 24 h of drought stress， gene expression levels were higher than those at 6 h and 0 h， and most of these genes were highly expressed in the leaves. These genes responded more strongly in roots and stems under severe drought， while they had higher expression levels in leaves under mild drought. In summary， the MYB-related gene family of A. bracteata exhibited distinct tissue-specific expression patterns under drought stress， laying a theoretical foundation for subsequent functional research and stress resistant breeding.

Key words: Atraphaxis bracteata, MYB-related, Drought stress, Expression pattern

中图分类号:

何彩, 刘源, 晋敏, 吴媛, 王素桢, 胡芳. 沙木蓼MYB-related基因家族的鉴定及其响应干旱胁迫的表达模式研究[J]. 草地学报, 2026, 34(6): 2012-2021.

HE Cai, LIU Yuan, JIN Min, WU Yuan, WANG Su-zhen, HU Fang. Identification of MYB-related Gene Family in Atraphaxis bracteata and Study on its Expression Pattern in Response to Drought Stress[J]. Acta Agrestia Sinica, 2026, 34(6): 2012-2021.

参考文献

[1] KLEMPNAUER K H，GONDA T J，MICHAEL BISHOP J. Nucleotide sequence of the retroviral leukemia gene v-myb and its cellular progenitor c-myb： the architecture of a transduced oncogene[J]. Cell，1982，31（2）：453-463
[2] PAZ-ARES J，GHOSAL D，WIENAND U，et al. The regulatory c1 locus of Zea mays encodes a protein with homology to myb proto-oncogene products and with structural similarities to transcriptional activators[J]. EMBO Journal，1987，6（12）：3553-3558
[3] STRACKE R，WERBER M，WEISSHAAR B. The R2R3-MYB gene family in Arabidopsis thaliana[J]. Current Opinion in Plant Biology，2001，4（5）：447-456
[4] KATIYAR A，SMITA S，LENKA S K，et al. Genome-wide classification and expression analysis of MYB transcription factor families in rice and Arabidopsis[J]. BioMed Central Genomics，2012，13：544
[5] OGATA K，MORIKAWA S，NAKAMURA H，et al. Comparison of the free and DNA-complexed forms of the DNA-binding domain from c-Myb[J]. Nature Structural & Molecular Biology，1995，2（4）：309-320
[6] JIN H，MARTIN C. Multifunctionality and diversity within the plant MYB-gene family[J]. Plant Molecular Biology，1999，41（5）：577-585
[7] DUBOS C，STRACKE R，GROTEWOLD E，et al. MYB transcription factors in Arabidopsis[J]. Trends in Plant Science，2010，15（10）：573-581
[8] ROSINSKI J A， ATCHLEY W R. Molecular evolution of the Myb family of transcription factors： evidence for polyphyletic origin[J]. Journal of Molecular Evolution，1998，46（1）：74-83
[9] STRACKE R，JAHNS O，KECK M，et al. Analysis of PROCUCTION OF FLAVONOL GLYCOSIDES-dependent flavonol glycoside accumulation in Arabidopsis thaliana plants reveals MYB11-，MYB12- and MYB111-independent flavonol glycoside accumulation[J]. New Phytologist，2010，188（4）：985-1000
[10] LIU Y H， ZENG Y T， LI Y M， et al. Genomic survey and gene expression analysis of the MYB-related transcription factor superfamily in potato （Solanum tuberosum L.）[J]. International Journal of Biological Macromolecules，2020，164：2450-2464
[11] ZHANG X Q，DONG B Y，SONG Z H，et al. Identification of MYB-related subfamily genes in Cajanus cajan and functional analysis of CcMYB-R48 under drought stress[J]. Journal of Agricultural Biotechnology，2022，30（9）：1698-1712 张修齐，董碧莹，宋治华，等. 木豆MYB-related亚家族基因鉴定及干旱胁迫下CcMYB-R48的功能解析[J]. 农业生物技术学报，2022，30（9）：1698-1712
[12] YAO F，JIANG M Y，YANG Y S，et al. Bioinformatics and expression analysis on MYB-related family in Angelica dahurica var. formosana[J]. China Journal of Chinese Materia Medica，2022，47（7）：1831-1846 姚菲，江美彦，杨云舒，等. 川白芷MYB-related基因家族的生物信息及表达模式分析[J]. 中国中药杂志，2022，47（7）：1831-1846
[13] RAN D. Genome-wide analysis of the MYB gene family in tung tree （Vernicia fordii）[D]. Changsha：Central South University of Forestry & Technology，2019：12 冉舵. 油桐MYB转录因子家族的鉴定与功能特征研究[D]. 长沙：中南林业科技大学，2019：12
[14] YU E Y，KIM S E，KIM J H，et al. Sequence-specific DNA recognition by the Myb-like domain of plant telomeric protein RTBP1[J]. Journal of Biological Chemistry，2000，275（31）：24208-24214
[15] BARANOWSKIJ N，FROHBERG C，PRAT S，et al. A novel DNA binding protein with homology to Myb oncoproteins containing only one repeat can function as a transcriptional activator[J]. Embo Journal，1994，13（22）：5383-5392
[16] SCHAFFER R，LANDGRAF J， ACCERBI M，et al. Microarray analysis of diurnal and circadian-regulated genes in Arabidopsis[J]. The Plant Cell，2001，13（1）：113-123
[17] CHEN N，PAN L J，YANG Z，et al. A MYB-related transcription factor from peanut，AhMYB30，improves freezing and salt stress tolerance in transgenic Arabidopsis through both DREB/CBF and ABA-signaling pathways[J]. Frontiers in Plant Science，2023，14：1136626
[18] LIU Y，ZHANG Z S，FANG K，et al. Genome-wide analysis of the MYB-related transcription factor family in pepper and functional studies of CaMYB37 involvement in capsaicin biosynthesis[J]. International Journal of Molecular Sciences，2022，23（19）：11667
[19] YAN X，DING W J，WU X Y，et al. Insights into the MYB-related transcription factors involved in regulating floral aroma synthesis in sweet Osmanthus[J]. Frontiers in Plant Science，2022，13：765213
[20] HE C，JIN M，LIU W，et al. The metabolomics and transcriptomics of Atraphaxis bracteata reveal age-related molecular alterations under naturally high temperature and drought conditions[J]. Physiologia Plantarum，2024，176（1）：e14129
[21] HE C，LIU W，JIN M，et al. Analysis of full length transcriptome and resistance characteristics of Atraphaxis bracteata under drought[J]. Scientific Reports，2025，15：807
[22] ZHANG Z X，GUO X W，WANG Z W，et al. Identification and expression analysis of NAC gene family in Cerasus humilis[J]. Journal of Fruit Science，2023，40（2）：206-222 张忠鑫，郭夕雯，汪泽文，等. 欧李NAC基因家族的鉴定及表达分析[J]. 果树学报，2023，40（2）：206-222
[23] LUO J C，JIN Y R，YANG Y C，et al.Identification and expression analysis of TCP gene family members in orchardgrass[J]. Acta Agrestia Sinica，2024，32（6）：1682-1692 骆金婵，靳雅荣，杨煜琛，等. 鸭茅TCP基因家族成员鉴定及表达分析[J]. 草地学报，2024，32（6）：1682-1692
[24] JOANNA K，PAWEL M，DOROTA M，et al. The relationships between the isoelectric point and： length of proteins， taxonomy and ecology of organisms[J]. BioMed Central Genomics，2007，8：163
[25] ZHAO X，LI Y M，LI Y M，et al. Identification and analysis of R1-MYB gene family in Rheum palmatum L. based on full-length transcriptome sequencing[J]. Acta Pharmaceutica Sinica，2023，58（5）：1354-1363 赵霞，李元敏，李依民，等. 基于全长转录组测序的掌叶大黄R1-MYB基因家族鉴定分析[J]. 药学学报，2023，58（5）：1354-1363
[26] JIA G Q，LI B，ZHANG D F，et al. Cloning and characterization of a novel R1-MYB transcription factor in maize[J]. Progress in Natural Science，2009，19（9）：1089-1096
[27] WANG C P，CHEN J W，YAN L，et al. Cloning and expression analysis of R1-MYB transcription factor in Lycium ruthenicum[J]. Chinese Traditional and Herbal Drugs，2018，49（1）：203-210 王翠平，陈建伟，严莉，等. 黑果枸杞R1-MYB转录因子基因的克隆及表达分析[J]. 中草药，2018，49（1）：203-210
[28] FAN J S B，ZHONG X X，SHAN T Y，et al. Identification and analysis of 1R-MYB transcription factors regulating the phenylpropane biosynthesis pathway in Ranunculus Japonicus Thunb[J]. Plant Science Journal，2024，42（4）：478-487 樊金世博，钟欣欣，单婷玉，等. 毛茛中与苯丙烷生物合成途径相关的1R-MYB类转录因子的鉴定与分析[J]. 植物科学学报，2024，42（4）：478-487

沙木蓼MYB-related基因家族的鉴定及其响应干旱胁迫的表达模式研究

Identification of MYB-related Gene Family in Atraphaxis bracteata and Study on its Expression Pattern in Response to Drought Stress

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