草地学报 ›› 2026, Vol. 34 ›› Issue (1): 142-153.DOI: 10.11733/j.issn.1007-0435.2026.01.014

• 研究论文 • 上一篇    

外源三十烷醇对干旱胁迫下红豆草生长和生理特性的影响

郭佳雨, 南丽丽, 王利群, 何蓉, 任孟雨, 马香香, 田戈   

  1. 甘肃农业大学草业学院/草业生态系统教育部重点实验室/农业农村部饲草种质创新与新品种选育重点实验室, 甘肃 兰州 730070
  • 收稿日期:2025-01-20 修回日期:2025-04-01 发布日期:2025-12-24
  • 通讯作者: 南丽丽,E-mail:nanll@gsau.edu.cn
  • 作者简介:郭佳雨( 2001-),女,汉族,山西临汾人,硕士研究生,主要从事牧草栽培与育种研究,E-mail:1872341681@qq.com;
  • 基金资助:
    国家自然科学基金项目(32360339);财政部和农业农村部:国家现代农业产业技术体系项目(CARS-34)资助

Effects of Exogenous Triacontanol on the Growth and Physiological Characteristics of Sainfoin Under Drought Stress

GUO Jia-yu, NAN Li-li, WANG Li-qun, HE Rong, REN Meng-yu, MA Xiang-xiang, TIAN Ge   

  1. College of Pratacultural Science, Gansu Agricultural University/Key Laboratory of Grassland Ecosystem, Ministry of Education/Key Laboratory of Forage Gerplasm Innovation and New Variety Breeding of Ministry of Agriculture and Rural Affairs, Lanzhou, Gansu Province 730070, China
  • Received:2025-01-20 Revised:2025-04-01 Published:2025-12-24

摘要: 为探究外源三十烷醇(Triacontanol,TA)对干旱胁迫下红豆草(Onobrychis viciifolia)幼苗生长和生理特性的影响,以‘甘肃’红豆草(GS)、新品系P1及2049为材料,采用营养液砂培法,以-1.0 MPa聚乙二醇6000(T1)模拟干旱胁迫,同时对红豆草叶面喷施0(CK),2.5(T2),5.0(T3),7.5(T4),10.0(T5)和12.5(T6) μmol·L-1 TA,测定21项形态与生理指标,筛选TA最佳缓解浓度。结果表明,不同浓度TA均能缓解红豆草干旱胁迫,其中10.0 μmol·L-1 TA(T5)效果最佳。TA处理下各品种地上部株高、生物量及光合参数等指标较T1提升19%~454%;根冠比、渗透调节物质及相关抗氧化酶活性降低15%~89%。此外,主成分分析表明气孔导度、叶绿素a和类胡萝卜素这3个指标为关键调控指标;对红豆草抗旱性进行综合评价,其排名为T5>T6>T4>T3>T2>T1;品种响应差异表现为GS>P1>2049。表明TA通过调节生理生化特性增强红豆草的抗旱性,其中10.0 μmol·L-1 (T5)处理最佳,本研究为合理利用TA解决红豆草栽培中的干旱问题提供了参考。

关键词: 三十烷醇, 红豆草, 干旱胁迫, 生理指标, 光合参数

Abstract: To investigate the effects of exogenous triacontanol (TA) on the growth and physiological characteristics of sainfoin (Onobrychis viciifolia) seedlings under drought stress, three materials (‘Gansu’ sainfoin, new line P1, and 2049) were subjected to -1.0 MPa polyethylene glycol 6000 (T1) simulated drought stress using a nutrient solution sand culture method. Foliar sprays of TA at 0(CK), 2.5(T2), 5.0(T3), 7.5(T4), 10.0(T5), and 12.5(T6) μmol·L-1 were applied, and 21 morphological and physiological indicators were measured to identify the optimal TA concentration. Results demonstrated that all TA concentrations alleviated drought stress, with 10.0 μmol·L-1 TA (T5) exhibiting the greatest efficacy. Under T5 treatment, shoot height, biomass, and photosynthetic parameters increased by 19%-454% compared to T1, while root-shoot ratio, osmoregulatory substances, and antioxidant enzyme activities decreased by 15%-89%. Principal component analysis identified stomatal conductance, chlorophyll a, and carotenoids as key regulatory indicators. Comprehensive drought resistance evaluation ranked treatments as T5 > T6 > T4 > T3 > T2 > T1, with varietal responsiveness ordered as GS > P1 > 2049. These findings indicated that TA enhanced sainfoin drought resistance by modulating physio-biochemical traits, with 10.0 μmol·L-1 (T5) being the optimal treatment, providing a theoretical basis for TA application in drought management during sainfoin cultivation.

Key words: Triacontanol, Sainfoin, Drought Stress, Physiological indicators, Photosynthetic parameters

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