关键词: &lsquo, 龙牧806&rsquo, 苜蓿, ⁶⁰Co-&gamma, 辐照, 生长和生理, 细胞形态结构

Abstract:

In order to investigate the changes of seedling growth, leaf physiology and leaf cell morphology and structure of Medicago sativa L.×Meliloides ruthenica (L.) Sojak. ‘Longmu 806’ dry seed were irradiated with different doses of ⁶⁰Co-γ radiated. The results showed that radiation had different effects on the growth, leaf physiology and leaf cell morphology of Medicago sativa L.×Meliloides ruthenica (L.) Sojak. ‘Longmu 806’ seedlings. With the increase of radiation dose, Plant height and root/shoot ratio of seedlings increased first and then decreased. The change of taproot length and biomass decreased. 1 200~1 500 Gy radiation doses had a stronger inhibitory effect on seedling growth. After radiation, soluble protein, soluble sugar and proline content increased first and then decreased. The content of malondialdehyde and relative electrical conductivity increased with the increase in radiation dose. The effect of 600~900 Gy radiations on leaf cell and chloroplast morphology was relatively small. When the radiation dose was 1 200~1 500 Gy, the cell morphology of leaves showed obvious deformation, vacuolization and plasmolysis, and thylakoid were severely deformed, the number of starch granules was large and the number of was large butthe number of mitochondria showed a significant decrease.

Key words: Medicago sativaL.×Meliloides ruthenica(L.) Sojak. ‘Longmu 806’, ⁶⁰Co-&gamma, radiation, Growth and physiology, Cell morphology structure