不同磷水平下幼套球囊霉与禾草内生真菌对多年生黑麦草生长的影响

doi:10.11733/j.issn.1007-0435.2018.06.025

草地学报 ›› 2018, Vol. 26 ›› Issue (6): 1458-1466.DOI: 10.11733/j.issn.1007-0435.2018.06.025

不同磷水平下幼套球囊霉与禾草内生真菌对多年生黑麦草生长的影响

郭艳娥^1,2, 李应德¹, 高萍¹, 汪治刚¹, 段廷玉¹

1. 兰州大学草地农业科技学院, 甘肃兰州 730020;
2. 兰州新区市政投资管理集团有限公司, 甘肃兰州新区 730300

收稿日期:2018-03-22 修回日期:2018-12-01 出版日期:2018-12-15 发布日期:2019-01-28
通讯作者: 段廷玉
作者简介:郭艳娥(1991-),女,甘肃会宁人,硕士研究生,主要从事牧草病理学研究,E-mail:guoye15@lzu.edu.cn
基金资助:
国家绿肥产业技术体系（CARS-22）资助

Effects of Claroideoglomus etunicatum and Grass Endophyte on the Growth of Lolium perenne under Different Phosphorus Levels

GUO Yan-e^1,2, Li Ying-de¹, GAO Ping¹, WANG Zhi-gang¹, DUAN Ting-yu¹

1. College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou, Gansu Province 730020, China;
2. Lanzhou New Area Municipal Investment Group Co., Ltd, Lanzhou New Area, Gansu Province 730300, China

Received:2018-03-22 Revised:2018-12-01 Online:2018-12-15 Published:2019-01-28

摘要/Abstract

摘要： 通过盆栽试验探究了4个磷（P）水平（0、10 mg·kg^-1、20 mg·kg^-1、30 mg·kg^-1）下，丛枝菌根真菌（arbuscular mycorrhizal fungus，AMF）幼套球囊霉（Claroideoglomus etunicatum）与禾草内生真菌（Epichloё）对多年生黑麦草（Lolium perenne）生长、养分吸收、光合特性和磷酸酶活性的影响。供试多年生黑麦草植株分别由含有禾草内生真菌（E⁺）和不含禾草内生真菌（E^-）的种子建植获得。结果表明：AMF对植物生长的促生效应可通过适量P添加得到加强；内生真菌增加了黑麦草的生物量和养分含量，并与AMF间存在交互效应，抑制其侵染，侵染率降低15.85%~20.03%；二者共同作用（AMF⁺E⁺）的地上生物量较AMF^-E⁺降低1.45%~17.74%，P₀和P₁₀（10 mg·kg^-1）水平下高于AMF⁺E^-处理，P₂₀（20 mg·kg^-1）和P₃₀（30 mg·kg^-1）相反。对全P含量的影响与对生物量的影响趋势基本一致。AMF⁺E⁺处理的净光合速率、蒸腾速率和气孔导度高于或介于AMF^-E⁺和AMF⁺E^-之间，磷酸酶活性低于或介于AMF^-E⁺和AMF⁺E^-之间。说明较适P水平加强了微生物效应，利于植物生长。

关键词: 丛枝菌根真菌, 禾草内生真菌, 磷水平, 多年生黑麦草

Abstract: Pots based experiment was performed with perennial ryegrass (Lolium perenne ) seeds that infected with grass endophyte (E⁺) and without grass endophyte (E^-) to determine the effects of arbuscular mycorrhizal fungus (AMF) Claroideoglomus etunicatum and grass endophyte Epichloë on plant growth,nutrient absorption,photosynthetic characteristics and phosphatase activities under 0,10 mg·kg^-1,20 mg·kg^-1 and 30 mg·kg^-1 phosphorus levels. The results showed that:The promoting effects of AMF on plant growth can be enhanced by adding an appropriate amount of phosphorus. Grass endophyte increased biomass and nutrient contents of perennial ryegrass,and endophyte had interactions with AMF by inhibiting the infection of AMF,colonization decreased by 15.85~20.03%. The aboveground biomass of AMF⁺E⁺ treatment was 1.45%~17.74% lower than that of AMF^-E⁺,which was higher than that of AMF⁺E^- under P₀ and P₁₀(10 mg·kg^-1),lower than that of under P₂₀ (20 mg·kg^-1) and P₃₀(30 mg·kg^-1). The effect on total phosphorus content was basically the same trend as that of on biomass. The net photosynthetic rate,transpiration rate and stomatal conductance of AMF⁺E⁺ treatment were higher or between AMF^-E⁺ and AMF⁺E^-,and phosphatase activity was lower or between AMF^-E⁺ and AMF⁺E^-. The appropriate phosphorus level enhances the microbial effect,and beneficial to plant growth.

Key words: Arbuscular mycorrhizal fungus, Grass endophyte, Phosphorus level, Lolium perenne

中图分类号:

S543+.6

郭艳娥, 李应德, 高萍, 汪治刚, 段廷玉. 不同磷水平下幼套球囊霉与禾草内生真菌对多年生黑麦草生长的影响[J]. 草地学报, 2018, 26(6): 1458-1466.

GUO Yan-e, Li Ying-de, GAO Ping, WANG Zhi-gang, DUAN Ting-yu. Effects of Claroideoglomus etunicatum and Grass Endophyte on the Growth of Lolium perenne under Different Phosphorus Levels[J]. Acta Agrestia Sinica, 2018, 26(6): 1458-1466.

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参考文献

[1] Schachtman D P,Reid R J,Ayling S M. Phosphorus uptake by plants:from soil to cell[J]. Plant Physiology,1998,116(2):447-453
[2] Raghothama K. Phosphate acquisition[J]. Annual Review of Plant Biology,1999,50(1):665-693
[3] Holford I C R. Soil phosphorus:its measurement,and its uptake by plants[J]. Australian Journal of Soil Research,1997,35(2):227-240
[4] Schussler A,Schwarzott D,Walker C. A new fungal phylum,the Glomeromycota:phylogeny and evolution[J]. Mycological Research,2001,105(12):1413-1421
[5] Ipsilantis I,Sylvia D M. Interactions of assemblages of mycorrhizal fungi with two Florida wetland plants[J]. Applied Soil Ecology,2007,35(2):261-271
[6] Wu Q S,Liu C Y,Zhang D J,et al. Mycorrhiza alters the profile of root hairs in trifoliate orange[J]. Mycorrhiza,2016,26(3):237-247
[7] Shu B,Xia R X,Wang P. Differential regulation of Pht1 phosphate transporters from trifoliate orange (Poncirus trifoliata L. Raf) seedlings[J]. Scientia Horticulturae,2012,146(3):115-123
[8] 谢贤安. 丛枝菌根共生体磷信号转运受体的发现及其分子机制的研究[D]. 华中农业大学,2013
[9] Xie X,Weng B,Cai B,et al. Effects of arbuscular mycorrhizal inoculation and phosphorus supply on the growth and nutrient uptake of Kandelia obovata (Sheue,Liu & Yong) seedlings in autoclaved soil[J]. Applied Soil Ecology,2014,75:162-171
[10] Li X,Ren A,Han R,et al. Endophyte-mediated effects on the growth and physiology of Achnatherum sibiricum are conditional on both N and P availability[J]. Plos One,2012,7(11):e48010
[11] Malinowski D P,Brauer D K,Belesky D P. The endophyte Neotyphodium coenophialum affects root morphology of tall fescue grown under phosphorus deficiency[J]. Journal of Agronomy and Crop Science,1999,183(1):53-60
[12] Malinowski D P,Alloush G A,Belesky D P. Evidence for chemical changes on the root surface of tall fescue in response to infection with the fungal endophyte Neotyphodium coenophialum[J]. Plant and soil,1998,205(1):1-12
[13] Binet M N,Sage L,Malan C,et al. Effects of mowing on fungal endophytes and arbuscular mycorrhizal fungi in subalpine grasslands[J]. Fungal Ecology,2013,6(4):248-255
[14] Chuchou M,Guo B,An Z Q,et al. Suppression of mycorrhizal fungi in fescue by the Acremonium coenophialum endophyte[J]. Soil Biology and Biochemistry,1992,24(7):633-637
[15] Mülle J. Artificial infection by endophytes affects growth and mycorrhizal colonisation of Lolium perenne[J]. Functional Plant Biology,2003,30(4):419-424
[16] Omacini M,Eggers T,Bonkowski M,et al. Leaf endophytes affect mycorrhizal status and growth of co-infected and neighbouring plants[J]. Functional Ecology,2006,20(2):226-232
[17] Novas M V,Cabral D,Godeas A M. Interaction between grass endophytes and mycorrhizas in Bromus setifolius from Patagonia,Argentina[J]. Symbiosis,2005,40(40):23-30
[18] Liu Q,Parsons A J,Xue H,et al. Competition between foliar Neotyphodium lolii endophytes and mycorrhizal Glomus spp. fungi in Lolium perenne depends on resource supply and host carbohydrate content[J]. Functional Ecology,2011,25(4):910-920
[19] Larimer A L,Bever J D,Clay K. Consequences of simultaneous interactions of fungal endophytes and arbuscular mycorrhizal fungi with a shared host grass[J]. Oikos,2012,121(12):2090-2096
[20] García-parisi P A,Omacini M. Arbuscular mycorrhizal fungi can shift plant-soil feedback of grass-endophyte symbiosis from negative to positive[J]. Plant and soil,2017,419:13-23
[21] Ping Gao,Yan'e Guo,Yingde Li,et al. Effects of dual inoculation of AMF and rhizobium on alfalfa (Medicago sativa) root rot caused by Microdochium tabacinum[J]. Australasian Plant Pathology,2018,47:195-203
[22] Koske R E,Gemma J N. A modified procedure for staining roots to detect VA mycorrhizas[J]. Mycological Research,1989,92(4):486-488
[23] 曹丽霞,候伟峰,钱洁鑫,等. 菌根接种对羊草根围土壤主要养分与球囊霉素含量的影响[J]. 草地学报,2016,24(3):537-543
[24] Hajong S,Kumaria S,Tandon P. Comparative study of key phosphorus and nitrogen metabolizing enzymes in mycorrhizal and non-mycorrhizal plants of Dendrobium chrysanthum Wall. ex Lindl[J]. Acta Physiologiae Plantarum,2013,35(7):2311-2322
[25] Li H,Smith S E,Holloway R E,et al. Arbuscular mycorrhizal fungi contribute to phosphorus uptake by wheat grown in a phosphorus-fixing soil even in the absence of positive growth responses[J]. New Phytologist,2006,172(3):536-543
[26] Gamper H,Peter M,Jansa J,et al. Arbuscular mycorrhizal fungi benefit from 7 years of free air CO₂ enrichment in well-fertilized grass and legume monocultures[J]. Global Change Biology,2004,10(2):189-199
[27] 甄莉娜,周禾,于肯明,等. 丛枝菌根真菌与磷添加对成株与幼苗间生长关系的影响[J]. 草地学报,2015,23(4):823-829
[28] Malinowski D P,Alloush G A,Belesky D P. Leaf endophyte Neotyphodium coenophialum modifies mineral uptake in tall fescue[J]. Plant and Soil,2000,227(1):115-126
[29] Mack K,Rudgers J. Balancing multiple mutualists:asymmetric interactions among plants,arbuscular mycorrhizal fungi,and fungal endophytes[J]. Oikos,2008,117(2):310-320
[30] 王晓瑜,郭艳娥,冯希,等. AM真菌与禾草内生真菌对黑麦草抗旱性的影响[J]. 草业科学,2018,35(2):380-390
[31] Marschener H. Role of root growth,arbuscular mycorrhiza,and root exudates for the efficiency in nutrient acquisition[J]. Field Crops Research,1998,56(1-2):203-207
[32] 丁艳,韩卓,王泽港,等. 不同基因型玉米幼苗对低磷条件的响应[J]. 中国农学通报,2011,27(30):32-34
[33] McLachlan K D. Acid phosphatase activity of intact roots and phosphorus nutrition in plants. 2. Variations among wheat roots[J]. Australian Journal of Agricultural Research,1980,31(3):441-448
[34] Nanamori M,Shinano T,Wasaki J,et al. Low phosphorus tolerance mechanisms:phosphorus recycling and photosynthate partitioning in the tropical forage grass,Brachiaria hybrid cultivar Mulato compared with rice[J]. Plant and Cell Physiology,2004,45(4):460-469
[35] Yan X,Liao H,Trull M C,et al. Induction of a major leaf acid phosphatase does not confer adaptation to low phosphorus availability in common bean[J]. Plant Physiology,2001,125(4):1901-1911
[36] 张海伟,黄宇,叶祥盛,等. 低磷胁迫下甘蓝型油菜酸性磷酸酶对磷效率的贡献分析[J]. 中国科学:生命科学,2010,40(5):418-427
[37] 宋勇春,李晓林,冯固. 菌根真菌磷酸酶活性对红三叶草生境中土壤有机磷亏缺的影响[J]. 生态学报,2001,21(7):1130-1135
[38] Koide R T,Kabir Z. Extraradical hyphae of the mycorrhizal fungus Glomus intraradices can hydrolyse organic phosphate[J]. New Phytologist,2000,148(3):511-517
[39] Javot H,Pumplin N,Harrison M J. Phosphate in the arbuscular mycorrhizal symbiosis:transport properties and regulatory roles[J]. Plant Cell and Environment,2007,30(3):310-322
[40] Hegeman C E,Grabau E A. A novel phytase with sequence similarity to purple acid phosphatases is expressed in cotyledons of germinating soybean seedlings[J]. Plant Physiology,2001,126(4):1598-1608
[41] Li C,Gui S,Yang T,et al. Identification of soybean purple acid phosphatase genes and their expression responses to phosphorus availability and symbiosis[J]. Annals of Botany,2012,109(1):275-285
[42] Sánchez-blanco M J,Ferrández T,Morales M A,et al. Variations in water status,gas exchange,and growth in Rosmarinus officinalis plants infected with Glomus deserticola under drought conditions[J]. Journal of Plant Physiology,2004,161(6):675-682
[43] Wang W H,Min L I,Liu R J,et al. Effects of Arbuscular Mycorrhizal Fungi on Some physiological Index of Zingiber officinace Rosc[J]. Journal of Laiyang Agricultural College,2003,20(3):175-177
[44] 郑舜怡,郭世荣,张钰,等. 丛枝菌根真菌对辣椒光合特性及根际微生物多样性和酶活性的影响[J]. 西北植物学报,2014,34(4):800-809
[45] 孙鹏飞,郭绍霞. AM真菌对薰衣草光合作用和生长的影响[J].青岛农业大学学报(自然科学版),2017,34(04):235-239
[46] 郭涛,申鸿,彭思利,等. 氮、磷供给水平对丛枝菌根真菌生长发育的影响[J]. 植物营养与肥料学报,2009,15(3):690-695
[47] 初亚男,张海波,秦泽峰,等. AM真菌与非菌根植物的相互作用关系[J]. 应用生态学报,2018,29(1):321-326
[48] Baird J M,Walley F L,Shirtliffe S J. Arbuscular mycorrhizal fungi colonization and phosphorus nutrition in organic field pea and lentil[J]. Mycorrhiza,2010,20(8):541-549
[49] 冯海艳,冯固,王敬国,等. 植物磷营养状况对丛枝菌根真菌生长及代谢活性的调控[J]. 菌物学报,2003,22(4):589-598
[50] Chen B,Roos P,Borggaard O K,et al. Mycorrhiza and root hairs in barley enhance acquisition of phosphorus and uranium from phosphate rock but mycorrhiza decreases root to shoot uranium transfer[J]. New Phytologist,2005,165(2):591-598
[51] Schardl C L,Phillips T D. Protective grass endophytes. Where are they from and where are they going?[J]. Plant Disease,1997,81(5):430-438
[52] 刘慧,陈薇,周勇,等. 内生真菌和丛枝菌根真菌对羊草生长的影响[J]. 植物生态学报,2015,39(5):477-485
[53] Omacini M,Semmartin M,Pérez L I,et al. Grass-endophyte symbiosis:A neglected aboveground interaction with multiple belowground consequences[J]. Applied Soil Ecology,2012,61(5):273-279
[54] Rahman M H,Saiga S. Endophytic fungi (Neotyphodium coenophialum) affect the growth and mineral uptake,transport and efficiency ratios in tall fescue (Festuca arundinacea)[J]. Plant and soil,2005,272(1):163-171
[55] Blanke V,Renker C,Wagner M,et al. Nitrogen supply affects arbuscular mycorrhizal colonization of Artemisia vulgaris in a phosphate-polluted field site[J]. New Phytologist,2005,166(3):981-992

不同磷水平下幼套球囊霉与禾草内生真菌对多年生黑麦草生长的影响

Effects of Claroideoglomus etunicatum and Grass Endophyte on the Growth of Lolium perenne under Different Phosphorus Levels

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