混播比例和刈割茬次对多花黑麦草+箭筈豌豆混播草地土壤细菌群落的影响

doi:10.11733/j.issn.1007-0435.2019.05.014

草地学报 ›› 2019, Vol. 27 ›› Issue (5): 1222-1230.DOI: 10.11733/j.issn.1007-0435.2019.05.014

混播比例和刈割茬次对多花黑麦草+箭筈豌豆混播草地土壤细菌群落的影响

苟文龙¹, 李平², 董臣飞³, 李达旭², 白史且², 师尚礼¹

1. 甘肃农业大学草业学院, 甘肃兰州 730070;
2. 四川省草原科学研究院, 四川成都 611731;
3. 江苏省农业科学院畜牧研究所, 江苏南京 210014

收稿日期:2019-07-26 修回日期:2019-09-12 出版日期:2019-10-15 发布日期:2019-11-09
通讯作者: 师尚礼
作者简介:苟文龙(1975-),男,陕西咸阳人,副研究员,博士研究生,主要从事牧草栽培研究,E-mail:514240346@qq.com
基金资助:
四川省重点研发面上项目（2019YFN0137）；国家重点研发项目（2017YFD0502）资助

Effects of Mixture Sowing Ratio and Cutting Time on the Soil Bacterial Community in Annual Ryegrass and Common Vetch Mixture

GOU Wen-long¹, LI Ping², Dong Chen-fei³, Li Da-xu², BAI Shi-qie², SHI Shang-li¹

1. College of Pratacultura Science, Gansu Agricultural University, Lanzhou, Gansu Province 730070, China;
2. Sichuan Academy of Grassland Sciences, Chengdu, Sichuan Province 611731, China;
3. Institute of Animal Science, Jiangsu Academy of Agricultural Sciences, Nanjing, Jiangsu Province 210014, China

Received:2019-07-26 Revised:2019-09-12 Online:2019-10-15 Published:2019-11-09

摘要/Abstract

摘要： 为探讨混播比例和刈割茬次对一年生禾豆混播草地土壤细菌群落的影响，本研究设多花黑麦草（Lolium multiflorum Lamk.）100%（R₁）、75%多花黑麦草+25%箭筈豌豆（Vicia sativa L.）（R₂）、50%多花黑麦草+50%箭筈豌豆（R₃）、25%多花黑麦草+75%箭筈豌豆（R₄）与100%箭筈豌豆（R₅）5个处理，分别于2017年1月4日（H₁）、3月14日（H₂）、4月21日（H₃）和5月19日（H₄）4个时期刈割，并采集0~20 cm土壤样本，分析土壤细菌群落组成。结果表明，变形菌门（Proteobacteria）、放线菌门（Actinobacteria）、厚壁菌门（Firmicutes）、拟杆菌门（Bacteroidetes）、酸杆菌门（Acidobacteria）、芽单胞菌门（Gemmatimonadetes）、硝化螺旋菌门（Nitrospirae）、浮霉菌门（Planctomycetes）、绿弯菌门（Chloroflexi）和疣微菌门（Verrucomicrobia）是混播草地主要土壤细菌群落。禾豆混播比例对土壤alpha多样性无显著性影响，刈割茬次对土壤细菌操作分类单元（Operational Taxonomic Unit，OTU）和物种丰富度指标（Chao 1指数）影响效果显著（P<0.05），改变了细菌群落结构。刈割茬次引起土壤细菌群落变化，影响了多花黑麦草和箭筈豌豆混播草地土壤细菌群落结构的均匀性。

关键词: 多花黑麦草, 箭筈豌豆, 混播比例, 刈割茬次, 土壤, 细菌群落

Abstract: This study was aimed to determine the effects of grass/legume ratio and cutting time on soil bacterial community of grass-legume mixture grassland. Fifteen plots were sowed in September 2016,with five treatments (100% annual ryegrass (Lolium multiflorum Lam.) (R₁),75% annual ryegrass + 25% common vetch (Vicia sativa L.) (R₂),50% annual ryegrass + 50% common vetch (R₃),25% annual ryegrass + 75% common vetch (R₄) and 100% common vetch (R₅)). Samples of 0~20 cm soils from each plot in 2017 were collected after mixture forages harvested at H₁(January 4),H₂ (March 14),H₃ (April 21) and H₄ (May 19),respectively.Proteobacteria),Actinobacteria,Firmicutes,Bacteroidetes,Acidobacteria,Gemmatimonadetes,Nitrospirae,Planctomycetes and Verrucomicrobia were the main soil bacterial phyla in annual ryegrass and common vetch mixtures. Ratio of grass/legume had no effects on soil bacterial alpha diversity,Cutting time significantly affected soil bacteria OTU and Chao 1 index (P<0.05),changed the bacterial community structure. Thus,cutting time could induce changes in soil bacterial community and influence the uniformity of the bacterial community structure in annual ryegrass and common vetch mixture.

Key words: Annual ryegrass, Common vetch, Mixture sowing ratio, Cutting time, Soil, Bacterial community

中图分类号:

苟文龙, 李平, 董臣飞, 李达旭, 白史且, 师尚礼. 混播比例和刈割茬次对多花黑麦草+箭筈豌豆混播草地土壤细菌群落的影响[J]. 草地学报, 2019, 27(5): 1222-1230.

GOU Wen-long, LI Ping, Dong Chen-fei, Li Da-xu, BAI Shi-qie, SHI Shang-li. Effects of Mixture Sowing Ratio and Cutting Time on the Soil Bacterial Community in Annual Ryegrass and Common Vetch Mixture[J]. Acta Agrestia Sinica, 2019, 27(5): 1222-1230.

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参考文献

[1] 郭丰源,王博文,李文鹏,等. 小麦-玉米轮作体系中土壤细菌菌群结构及多样性分析[J]. 麦类作物学报,2018,38(05):623-630
[2] 王岳坤,洪葵. 红树林土壤细菌群落16S rDNA V3片段PCR产物的DGGE分析[J]. 微生物学报,2005,45(2):201-204
[3] Kulmatiski A,Beard K H,Stevens J R,et al. Plant-soil feedbacks:A meta-analytical review[J]. Ecology Letters,2008,11(9):980-992
[4] Zhou J,Xia B,Treves D S,et al. Spatial and resource factors influencing high microbial diversity in soil[J]. Apply Environ Microbial,2002,68(1):326-334
[5] 杨广容,马燕,蒋宾,等. 基于16S rDNA测序对茶园土壤细菌群落多样性的研究[J]. 生态学报,2019,39(22):1-10
[6] Lauber C L,Hanady M,Knight R,et al. Pyrosequencing-based assessment of soil pH as a predictor of soil bacterial community structure at the continental scale[J]. Applied and Environmental Microbiology,2009,75(15):5111-5120
[7] Frankow-Lindberg B E,Halling M,Hoglind M,et al. Yield and stability of yield of single and multi-clover grass-clover swards in two contrasting temperate environments[J]. Grass and Forage Science,2009,64(3):236-245
[8] Nguyen C. Rhizodeposition of organic C by plants:mechanisms and controls[J]. Agronomie,2003,23(5-6):375-396
[9] 韩建国,马春晖,毛培胜等. 播种比例和施氮量及刈割期对燕麦与豌豆混播草地产草量和质量的影响[J]. 草地学报,1999,7(2):87-94
[10] 张永亮,王建丽,胡自治. 杂花苜蓿与无芒雀麦混播群落种间竞争及稳定性[J]. 草地学报,2007,15(1):43-49
[11] 张永亮,范富,高凯,等. 苜蓿、无芒雀麦单播与混播对土壤有机质和速效养分的影响[J]. 草地学报,2009,17(1):22-26
[12] 朱亚琼,郑伟,王祥,等. 混播方式对豆禾混播草地植物根系构型特征的影响[J]. 草业学报,2018,27(1):73-85
[13] 郭海明,于磊,林祥群. 施肥对绿洲区苜蓿与无芒雀麦混播草地生产性能的影响[J]. 石河子大学学报,2009,27(1):46-50
[14] Trannin W,Urquiaga S,Guerra G,et al. Interspecies competition and N transfer in a tropical grass-legume mixture[J]. Biology and Fertility of Soils,2000,32(6):441-448
[15] 陆炳章. 黑麦草与豆科绿肥混播改土效果及其应用[J]. 草业科学,1985,2(2):43-46
[16] 王旭,曾昭海,朱波,等. 燕麦与箭筈豌豆不同混作模式对根际土壤微生物数量的影响[J]. 草业学报,2009,18(6):151-157
[17] Zeng Q,An S,Liu Y. Soil bacterial community response to vegetation succession after fencing in the grassland of China[J]. Science of the Total Environment,2017,609:2-10
[18] Mohammad Bahram,Falk Hildebrand,Sofia K,et al. Structure and function of the global topsoil microbiome[J]. Nature,2018,560:233-237
[19] Mago? T,Salzberg S L. FLASH:fast length adjustment of short reads to improve genome assemblies[J]. Bioinformatics,2011,27(21):2957-2963
[20] Bokulich N A,Subramanian S,Faith J J,et al. Quality-filtering vastly improves diversity estimates from Illumina amplicon sequencing[J]. Nature Methods,2012.10:57-59
[21] Haas B. J,G D E A. Chimeric 16S rRNA sequence formation and detection in Sanger and 454-pyrosequenced PCR amplicons[J]. Genome Research,2011,21:494-504
[22] Edgar R C. UPARSE:highly accurate OTU sequences from microbial amplicon reads[J]. Nature Methods,2013,10:996-998
[23] Desantis T Z,Hugenholtz P,Larsen N,et al. Greengenes,a Chimera-Checked 16S rRNA Gene Database and Workbench Compatible with ARB[J]. Applied and Environmental Microbiology,2006,72(7):5069-5072
[24] Wang Q,Garrity G M,Tiedje J M,et al. Naive Bayesian classifier for rapid assignment of rRNA sequences into the new bacterial taxonomy[J]. Applied and environmental microbiology,2007,73(16):5261-5267
[25] Edgar R C. MUSCLE:multiple sequence alignment with high accuracy and high throughput[J]. Nucleic Acids Research,2004,32(5):1792-1797
[26] Gao S,Zhang R,Cao W,et al. Long-term rice-rice-green manure rotation changing the microbial communities in typical red paddy soil in South China[J]. Journal of Integrative Agriculture,2015,14(12):2512-2520
[27] Lu X,Ji S,Hou C,et al. Impact of root C and N reserves on shoot regrowth of defoliated alfalfa cultivars differing in fall dormancy[J]. Grassland Science,2018,64(2):83-90
[28] Lu X,Ji S,Hou C,et al. Morphological development and dry weight distribution of alfalfa cultivars varying in fall dormancy under a short-term cultivation system[J]. Grassland Science,2017,63(1):23-28
[29] Ding J,Jiang X,Ma M,et al. Effect of 35 years inorganic fertilizer and manure amendment on structure of bacterial and archaeal communities in black soil of northeast China[J]. Applied Soil Ecology,2016,105:187-195
[30] Habekost M,Eisenhauer N,Scheu S,et al. Seasonal changes in the soil microbial community in a grassland plant diversity gradient four years after establishment[J]. Soil Biology and Biochemistry,2008,40(10):2588-2595
[31] 芦奕晓,牟乐,杨惠敏. 豆科与禾本科牧草混播改良土壤的研究进展[J]. 中国草地学报,2019,41(1):94-100
[32] Thakur M P,Milcu A,Manning P,et al. Plant diversity drives soil microbial biomass carbon in grasslands irrespective of global environmental change factors[J]. Global Change Biology,2015,21:4076-4085
[33] Zhao J,Zeng Z,He X,et al. Effects of monoculture and mixed culture of grass and legume forage species on soil microbial community structure under different levels of nitrogen fertilization[J]. European Journal of Soil Biology,2015,68:61-68
[34] Mikkonen A,Kondo E,Lappi K,et al. Contaminant and plant-derived changes in soil chemical and microbiological indicators during fuel oil rhizoremediation with Galega orientalis[J]. Geoderma,2011,160(3):336-346
[35] 章家恩,刘文高,陈景青,等. 刈割对牧草地下部根区土壤养分及土壤酶活性的影响[J]. 生态环境,2005,14(3):387-391
[36] 朱丽霞,章家恩,刘文高. 根系分泌物与根际微生物相互作用研究综述[J]. 生态环境,2003,12(1):102-105
[37] 赛吉日呼.牧草不同种植方式对土壤酶活性和土壤微生物量及多样性的影响[M]. 呼和浩特:内蒙古大学,2019:41-42
[38] 苟文龙,李平,张建波,等. 多花黑麦草+箭筈豌豆混播草地地上生物量和营养品质动态研究[J]. 草地学报,2019,27(2):473-481

混播比例和刈割茬次对多花黑麦草+箭筈豌豆混播草地土壤细菌群落的影响

Effects of Mixture Sowing Ratio and Cutting Time on the Soil Bacterial Community in Annual Ryegrass and Common Vetch Mixture

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