关键词: 沟叶结缕草, 温度胁迫, 蛋白质组学

Abstract: The physiological mechanisms of cold-resistance and heat-tolerance in Zoysia matrella,as well as its maintenance management,have been reported,however little information is available for its resistance pertaining to molecular mechanisms.The differential expression proteins of leaves in the turfgrass under low temperature and high temperature treatments were studied by the method of differential proteomics,to search for molecular mechanisms of Z.matrella which was relatively tolerant to high temperature while sensitive to chilling.There were 35 protein spots showing different expression patterns in the turfgrass after treated with different temperature stress.Twenty six proteins were successfully identified.Further analysis showed that some of the expressed proteins were up-regulated under the low temperature treatment,such as functioning to increase signal transduction and activate oxygen scavenging and protein defense,would enhance a resistance ability in Z.matrella to decrease low temperature stress.Also,some proteins were found to be down-regulated in Z.matrella under the low temperature treatment.Those down-regulated proteins resulted in an initial increase,and then followed by a decrease of respiration rate.In turn,photosythesis declined,proteins degraded,and transportation ability reduced,consequently led to decreased resistance potential in Z.matrella under the low temperature treatment.These up-regulated proteins strengthened signal discernableness and its transduction,increased photosythesis and respiration,promoted secondary metabolism,and activated oxygen scavenging.They enhanced protein defense and synthesis,in turn,led to a stronger resistance capability in Z.matrella under the high temperature treatment.

Key words: Zoysia matrella, Temperature stress, Proteomics