干旱胁迫对2种不同抗旱性老芒麦幼苗ROS积累及抗氧化系统的影响

doi:10.11733/j.issn.1007-0435.2020.03.012

草地学报 ›› 2020, Vol. 28 ›› Issue (3): 684-693.DOI: 10.11733/j.issn.1007-0435.2020.03.012

干旱胁迫对2种不同抗旱性老芒麦幼苗ROS积累及抗氧化系统的影响

杨伟^1,2, 刘文辉¹, 马祥¹, 马晖玲²

1. 青海省青藏高原优良牧草种质资源利用重点实验室/青海省畜牧兽医科学院, 青海西宁 810016;
2. 甘肃农业大学草业学院, 甘肃兰州 730070

收稿日期:2019-12-10 修回日期:2020-01-28 出版日期:2020-06-15 发布日期:2020-05-30
通讯作者: 马晖玲
作者简介:杨伟(1992-),男,汉,甘肃兰州人,博士研究生,研究方向为牧草种质资源与育种,E-mail:1365054954@qq.com;
基金资助:
青海省科技厅重点实验室发展专项“青海省青藏高原优良牧草种质资源利用重点实验室”（2020-ZJ-Y09）资助

Effects of ROS Accumulation and Antioxidant System in Two Different Drought Resistant Elymus sibiricus under Drought Stress

YANG Wei^1,2, LIU Wen-hui¹, MA Xiang¹, MA Hui-ling²

1. Key Laboratory of Superior Forage Germplasm in the Qinghai-Tibetan Plateau/Qinghai Academy of Animal Science and Veterinary medicine, Xining, Qinghai Province 810016, China;
2. College of Pratacultural Science, Gansu Agricultural University, Lanzhou, Gansu Province 730070, China

Received:2019-12-10 Revised:2020-01-28 Online:2020-06-15 Published:2020-05-30

摘要/Abstract

摘要： 为探究不同抗旱性老芒麦（Elymus sibiricus L.）材料对干旱胁迫下幼苗活性氧（Reactive oxygen species，ROS）积累和清除的影响，从而为老芒麦抗旱生理机制的研究及抗旱材料的选育提供一定的理论基础，本试验选用抗旱性差异明显的2份老芒麦材料（抗旱性强的材料，记为Q，处理组QD，对照组QC；抗旱性弱的材料，记为S，处理组SD，对照组SC），采用砂培法培养材料，待幼苗长到2叶1心时，用含有20% PEG 6000（W/V）的1/2 Hoagland营养液进行胁迫处理，2份材料的对照正常培养。在处理7 d、14 d和21 d取样，分别测定各项指标。试验结果表明，在整个胁迫过程中，QD的株高、叶片相对含水量（Relative Water Content，RWC）降幅及ROS和丙二醛（Malondialdehyde，MDA）增幅均低于SD，但是抗氧化酶活性的增幅以及抗氧化物质的比值高于SD。与各自的对照相比，干旱胁迫21 d后，QD和SD的株高分别下降39.11%和55.20%；RWC分别下降78.34%和84.52%；O₂^·-含量分别增加235.71%和323.03%；H₂O₂含量分别增加126.19%和234.88%；MDA含量分别增加318.99%和434.81%；超氧化物歧化酶（Superoxide dismutase，SOD）活性分别增加156.20%和83.83%；过氧化氢酶（Catalase，CAT）活性分别增加32.39%和30.50%；抗坏血酸过氧化物酶（Ascorbate Peroxidase，APX）活性分别增加58.10%和172.89%；脱氢抗坏血酸还原酶（Dehydroascorbate Reductase，DHAR）活性分别增加66.54%和11.38%；谷胱甘肽还原酶（Glutathione Reductase，GR）活性分别增加227.07%和130.29%；抗坏血酸（Ascorbic Acid，ASA）和脱氢抗坏血酸（Dehydroascorbic acid，DHA）比值（ASA/DHA）的降幅分别为52.51%和73.69%；谷胱甘肽（Glutathione，GSH）和氧化型谷胱甘肽（Glutathiol，GSSH）比值（GSH/GSSH）的降幅分别为40.94%和61.43%。综上可得，干旱胁迫下抗旱性强的材料株高和RWC降幅、ROS积累和膜脂过氧化程度显著低于抗旱性弱的材料；抗旱性强的老芒麦响应干旱胁迫时以SOD、CAT和GR为主，抗旱性弱的老芒麦响应干旱胁迫时以SOD、CAT和APX为主；但是整体来看，抗旱性强的老芒麦表现出更强的抗氧化防御系统。

关键词: 老芒麦, 干旱胁迫, 活性氧, 抗氧化物质

Abstract: To study the effects of different drought resistant Elymus sibiricus materials on the accumulation and removal of reactive oxygen species (ROS) in seedlings under drought stress,and to provide certain theoretical basis for the research on the physiological mechanism of drought resistant Elymus sibiricus and the selection and breeding of drought resistant materials. The seedlings of two Elymus sibiricus materials with significant differences (Q stands for the strong drought resistant material,S stands for the weak drought resistant material) in drought resistance were cultured with sand. When the seedlings grew to 2 leaves and 1 shoot,1/2 Hoagland nutrient solution containing 20% PEG 6000 (W/V) was used for stress treatment,and the two materials without PEG treatment were used as control. Samples were taken at 7 d,14 d and 21 d after treatment and the indexes were measured,respectively. The activity of antioxidant enzymes was also measured. The results showed that during the whole stress process,the reduction of plant height,relative water content (RWC) and the increase range of ROS,malondialdehyde (MDA) of QD were lower than that of SD,and the increase range of antioxidant enzyme activity and the ratio of antioxidant substance were higher than that of SD. Compared to their respective controls,the plant height of QD and SD decreased by 39.11% and 55.20%,respectively,after 21 days of drought stress treatment. The RWC has dropped by 78.34% and 84.52%. The O₂^·- content increased by 235.71% and 323.03%. H₂O₂ content increased by 126.19% and 234.88%. MDA content increased by 318.99% and 434.81%. Superoxide dismutase (SOD) activity increased by 156.20% and 83.83%. Catalase (CAT) activity increased by 32.39% and 30.50%. Ascorbate peroxidase (APX) activity increased by 58.10% and 172.89%. Dehydroascorbate reductase (DHAR) activity increased by 66.54% and 11.38%. Glutathione reductase (GR activity) increased by 227.07% and 130.29%. The decrease of ASA/DHA was 52.51% and 73.69%. The decrease of GSH/GSSH was 40.94% and 61.43%. In summary,the reduction of plant height,RWC and ROS accumulation and membrane lipid peroxidation degree of strong drought resistant material were lower than that of weak drought resistant material. In response to drought stress,SOD,CAT and GR were dominant in the Elymus sibiricus with strong drought resistance,while SOD,CAT and APX were dominant in the Elymus sibiricus with weak drought resistance. But overall,the strong drought-resistant Elymus sibiricus showed a stronger antioxidant defense system.

Key words: Elymus sibiricus L., Drought stress, ROS, Antioxidant substance

中图分类号:

Q543.9

杨伟, 刘文辉, 马祥, 马晖玲. 干旱胁迫对2种不同抗旱性老芒麦幼苗ROS积累及抗氧化系统的影响[J]. 草地学报, 2020, 28(3): 684-693.

YANG Wei, LIU Wen-hui, MA Xiang, MA Hui-ling. Effects of ROS Accumulation and Antioxidant System in Two Different Drought Resistant Elymus sibiricus under Drought Stress[J]. Acta Agrestia Sinica, 2020, 28(3): 684-693.

0
/ / 推荐

导出引用管理器 EndNote|Ris|BibTeX

链接本文: https://manu40.magtech.com.cn/Jweb_cdxb/CN/10.11733/j.issn.1007-0435.2020.03.012

https://manu40.magtech.com.cn/Jweb_cdxb/CN/Y2020/V28/I3/684

参考文献

[1] Douglas R,Dewey. Cytogenetics of Elymus sibiricus and Its Hybrids with Agropyrontauri,Elymuscanadensis,and Agropyroncaninum[J]. Botanical Gazette,1974,135(1):80-87
[2] 陈焘,南志标. 不同储存年限老芒麦种子种带真菌检测及致病性测定[J]. 草业学报,2015,24(2):96-103
[3] 张俊超,谢文刚,赵旭红,等. 老芒麦种子离区酶活变化及组织学分析[J]. 草业学报,2018,27(7):84-92
[4] 王明亚. 施氮对老芒麦种子产量及产量因子的作用[D]. 北京:中国农业大学,2018:23
[5] Hasanuzzaman Mirz,Fujita Masayuki. Selenium pretreatment upregulates the antioxidant defense and methylglyoxal detoxification system and confers enhanced tolerance to drought stress in rapeseed seedlings[J]. Biological Trace Element Research,2011,143(3):1758-1776
[6] Nahar K,Hasanuzzaman M,Alam M M,et al. Insights into spermine-induced combined high temperature and drought tolerance in mung bean:osmoregulation and roles of antioxidant and glyoxalase system[J]. Protoplasma,2017,254(1):445-460
[7] Im Moller,Pe Jensen,A Hansson. Oxidative modifications to cellular components in plants[J]. Annual Review of Plant Biology,2007,58(1):459-481
[8] 赵丽英,邓西平,山仑. 活性氧清除系统对干旱胁迫的响应机制[J]. 西北植物学报,2005,25(2):413-418
[9] Gill S S,Tuteja N. Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants[J]. Plant Physiol Biochem,2010,48(12):909-930
[10] 林琪,侯立白,韩伟,等. 干旱胁迫对小麦旗叶活性氧代谢及灌浆速率的影响[J]. 西北植物学报,2003,23(12):2152-2156
[11] 宋吉轩,李金还,刘美茹,等. 油菜素内酯对干旱胁迫下羊草渗透调节及抗氧化酶的影响研究[J]. 草业学报,2015,24(8):93-102
[12] 侯俊峰. 不同抗旱型小麦品种响应干旱差异的生理机理[D]. 郑州:河南农业大学,2018:14
[13] 张卫红,刘大林,苗彦军,等. 西藏3种野生牧草苗期对干旱胁迫的响应[J]. 生态学报,2017,37(21):7277-7285
[14] 孙清洋,李志勇,李鸿雁,等. 水分胁迫对老芒麦种质萌发及幼苗生理特性的影响[J]. 中国草地学报,2016,38(3):19-25
[15] 祁娟,徐柱,王海清,等. 披碱草与老芒麦苗期抗旱性综合评价[J]. 草地学报,2009,17(1):36-42
[16] 高俊凤. 植物生理学实验指导[M]. 北京:高等教育出版社,2006:221-223
[17] Liu Z J,Guo Y K,Bai J G, et al. Exogenous Hydrogen Peroxide Changes Antioxidant Enzyme Activity and Protects Ultrastructure in Leaves of Two Cucumber Ecotypes Under Osmotic Stress[J]. Journal of Plant Growth Regulation,2010,29(2):171-183
[18] Kampfenkel K,Vanmontagu M,Inze D, et al. Extraction and Determination of Ascorbate and Dehydroascorbate from Plant Tissue[J]. Analytical Biochemistry,1995,225(1):165-167
[19] 曹丹. 不同抗性玉米品种苗期干旱及复水过程中的生理响应[D]. 北京:中国科学院大学,2015:14-15
[20] 曾令霜,李培英,孙宗玖. 10份新疆狗牙根种质抗旱性评价[J]. 中国草地学报,2019,41(03):22-29
[21] 李格,孟小庆,蔡敬,等. 活性氧在植物非生物胁迫响应中功能的研究进展[J]. 植物生理学报,2018,54(06):951-959
[22] Apel K,Hirt H. REACTIVE OXYGEN SPECIES:Metabolism,Oxidative Stress,and Signal Transduction[J]. Annual Review of Plant Biology,2004,55(1):373-399
[23] Moller I M. Plant mitochondria and oxidative stress:Electron transport,NADPH turnover,and metabolism of reactive oxygen species[J]. Annual Review of Plant Biology,2001,52:561-591
[24] 王传旗,苗彦军,王建林,等. 西藏野生垂穗披碱草苗期抗旱性研究[J]. 中国草地学报,2017,39(4):116-120
[25] 石永红,万里强,刘建宁,等. 干旱胁迫对6个坪用多年生黑麦草品种抗旱性的影响[J]. 草地学报,2009(01):58-63
[26] 李丽杰,顾万荣,孟瑶,等. 干旱胁迫下亚精胺对玉米幼苗抗旱性影响的生理生化机制[J]. 应用生态学报,2018,29(2):554-564
[27] 叶君,邓西平,王仕稳,等. 干旱胁迫下褪黑素对小麦幼苗生长、光合和抗氧化特性的影响[J]. 麦类作物学报,2015,35(9):1275-1283
[28] 张铭. 不同花生品种对干旱胁迫与复水的响应及其机理[D]. 泰安:山东农业大学,2017:57-60
[29] 田又升,王志军,于航,等. 干旱胁迫对不同抗旱性棉花品种抗氧化酶活性及基因表达的影响[J]. 西北植物学报,2015,35(12):2483-2490
[30] 马文涛,樊卫国. 贵州野生柑橘的抗旱性及其活性氧代谢对干旱胁迫的响应[J]. 果树学报,2014,31(3):394-400
[31] 唐振鑫. 毛白杨脱氢抗坏血酸还原酶家族的功能分化研究[D]. 北京:北京林业大学,2013:3-4
[32] Kuiju Niu,Xiang Ma,Guoling Liang,et al. 5-Aminolevulinic acid modulates antioxidant defense systems and mitigates drought-induced damage in Kentucky bluegrass seedlings[J]. Protoplasma,2017,254(18):2083-2094
[33] Loggini B,Scartazza A,Navari-Izzo B F,et al. Antioxidative Defense System,Pigment Composition,and Photosynthetic Efficiency in Two Wheat Cultivars Subjected to Drought[J]. Plant Physiology,1999,119(3):1091-1099
[34] 郑清岭. 沙芥属植物干旱胁迫响应机制[D]. 呼和浩特:内蒙古农业大学,2018:78-80

干旱胁迫对2种不同抗旱性老芒麦幼苗ROS积累及抗氧化系统的影响

Effects of ROS Accumulation and Antioxidant System in Two Different Drought Resistant Elymus sibiricus under Drought Stress

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	佟莉蓉, 倪顺刚, 任星远, 刘嘉欣, 闻静, 王娟, 宋雨. 褪黑素对干旱胁迫下达乌里胡枝子幼苗生长及叶片水分生理的影响[J]. 草地学报, 2021, 29(8): 1682-1688.
[2]	张韦钰, 王春勇, 杜红梅. 富氢水对草地早熟禾耐盐性的影响以及与抗氧化酶活性的关系[J]. 草地学报, 2021, 29(7): 1436-1445.
[3]	李鹏珍, 赵得琴, 邓波, 杨军, 赵国华, 赵亮. 生物炭与干旱胁迫对接种紫花苜蓿光合效率及生长的影响[J]. 草地学报, 2021, 29(6): 1257-1264.
[4]	张荟荟, 梁维维, 张学洲, 阿斯娅·曼力克, 朱昊, 魏鹏, 康帅, 李学森. 新疆野生老芒麦种质资源形态及生长特性分析[J]. 草地学报, 2021, 29(4): 701-708.
[5]	梁佳, 马依努·吾斯曼, 方志刚. 外源生长物质对干旱胁迫条件下甜高粱种子萌发的影响[J]. 草地学报, 2021, 29(3): 610-617.
[6]	刘备, 宋玉梅, 孙铭, 毛培胜. 燕麦劣变种子吸胀过程中线粒体AsA-GSH循环的生理响应[J]. 草地学报, 2021, 29(2): 211-219.
[7]	宿敬龙, 祁娟, 刘艳君, 杨航, 李明. 不同氮素水平及施氮方式对老芒麦根际土壤特性的影响[J]. 草地学报, 2021, 29(2): 259-269.
[8]	韩梦丽, 白史且, 孙盛楠, 鄢家俊, 张传杰, 张昌兵, 张靖雪, 游明鸿, 李达旭, 严学兵. 基于MaxEnt模型青藏高原老芒麦适生区模拟预测[J]. 草地学报, 2021, 29(2): 374-382.
[9]	叶晓倩, 刘瑞曦, 冯金慧, 邓仕明, 李吉涛, 邓志军. 车前种子黏液对其劣变和萌发抗逆性的影响[J]. 草地学报, 2021, 29(12): 2728-2732.
[10]	余淑艳, 周燕飞, 黄薇, 王星, 高雪芹, 伏兵哲. 干旱、盐及旱盐复合胁迫对沙芦草光合和生理特性的影响[J]. 草地学报, 2021, 29(11): 2399-2406.
[11]	齐晨汐, 易观涛, 付晓璇, 王海龙, 王佳琳, 吴建慧. 丛枝菌根真菌对干旱胁迫下鹅绒委陵菜生理指标的影响[J]. 草地学报, 2021, 29(11): 2407-2412.
[12]	杨航, 祁娟, 刘文辉, 刘艳君, 宿敬龙, 李明. 异龄老芒麦根际土壤中脂肪酸类物质检测及根际土壤的自毒效应研究[J]. 草地学报, 2021, 29(1): 52-59.
[13]	秦立刚, 李雪, 李韦瑶, 胡尧, 李俊, 崔国文. PEG干旱胁迫对3种葱属植物种子萌发期渗透调节物质及酶活性的影响[J]. 草地学报, 2021, 29(1): 72-79.
[14]	张然, 马祥, 朱瑞婷, 牛奎举, 赵春旭, 马晖玲. 青海野生草地早熟禾响应干旱胁迫的代谢通路及转录调控分析[J]. 草地学报, 2020, 28(6): 1508-1518.
[15]	刘凯强, 刘文辉, 贾志锋, 马祥, 梁国玲. 干旱胁迫对‘青燕1号’燕麦器官生长及水分利用效率的影响[J]. 草地学报, 2020, 28(6): 1552-1562.