60Coγ辐射柱花草M3代的农艺性状及遗传多样性分析

doi:10.11733/j.issn.1007-0435.2021.09.016

草地学报 ›› 2021, Vol. 29 ›› Issue (9): 1992-2000.DOI: 10.11733/j.issn.1007-0435.2021.09.016

⁶⁰Coγ辐射柱花草M₃代的农艺性状及遗传多样性分析

罗铭欣¹, 刘凤民², 陈纪言^1,3, 崔均涛^3,4, 张伟丽^1,3

1. 仲恺农业工程学院农业与生物学院, 广东广州 510225;
2. 仲恺农业工程学院教学科研基地管理中心, 广东广州 510225;
3. 德庆仲恺农业产业科技创新研究有限公司, 广东肇庆 526642;
4. 仲恺农业工程学院园艺园林学院, 广东广州 510225

收稿日期:2021-03-03 修回日期:2021-04-12 出版日期:2021-09-15 发布日期:2021-10-14
通讯作者: 张伟丽,E-mail:zhangweili7218@163.com
作者简介:罗铭欣(1996-),女,广东德庆人,硕士研究生,主要从事作物遗传育种研究,E-mail:734486374@qq.com
基金资助:
广东省科技计划项目（2017A020208073）；广东省现代农业产业技术体系创新团队（2020KJ114）资助

Agronomic Traits and Genetic Diversity Analysis of M₃ Generation of Stylosanthes spp. Irradiated by ⁶⁰Coγ

LUO Ming-xin¹, LIU Feng-min², CHEN Ji-yan^1,3, CUI Jun-tao^3,4, ZHANG Wei-li^1,3

1. College of Agriculture and Biology, Zhongkai University of Agriculture and Engineering, Guangzhou, Guangdong Province 510225, China;
2. Management Center of Teaching and Research Base, Zhongkai University of Agriculture and Engineering, Guangzhou, Guangdong Province 510225, China;
3. Deqing Zhongkai Agricultural Tech. Innovation Research Co. Ltd., Zhaoqing, Guangdong Province 526642, China;
4. College of Horticulture and Landscape Architecture, Zhongkai University of Agriculture and Engineering, Guangzhou, Guangdong Province 510225, China

Received:2021-03-03 Revised:2021-04-12 Online:2021-09-15 Published:2021-10-14

摘要/Abstract

摘要： 为了探究辐射对柱花草（Stylosanthes spp.） M₃代农艺性状的影响及分子标记辅助诱变育种技术，利用经⁶⁰Coγ射线辐射诱变后的柱花草种子，种植后经过连续2代的单株选择筛选到17份性状优良的M₂代柱花草种子，种植成为M₃代后，对17份柱花草测定其株高、冠径、分枝数、第一分支长、病害级数、开花期、单株干重，并进行DNA保守序列多态性（Conserved DNA-derived polymorphism，CDDP）和相关序列扩增多态性（Sequence related amplified polymorphism，SRAP）的遗传多样性分析。运用NTSYS-pc 2.1软件计算17份柱花草的遗传相似系数，17条CDDP引物以及26对SRAP引物分别扩增出92条和177条带，其中多态性条带数分别为66条和111条，多态性比率分别为71.7%和62.7%，特异性条带各为13条和29条，特异性比率分别为14.1%和16.4%，种质间的遗传相似系数（GS）范围为0.54~0.90和0.60~0.94，平均遗传相似系数为0.76和0.79。按照非加权配对算术平均法基于CDDP标记和SRAP标记联合作17份柱花草的聚类图，在遗传相似系数为0.82时可将17份柱花草分成7个类群，聚类结果与农艺性状统计结果基本一致。本研究为今后辐射诱变柱花草育种提供一定的参考依据。

关键词: 柱花草, ⁶⁰Coγ射线, 农艺性状, CDDP, SRAP, 遗传多样性

Abstract: To explore the effects of radiation on the agronomic traits of Stylosanthes spp. M₃ generation and molecular marker-assisted mutation breeding technology, plant height, crown diameter, number of branches, first branch length, disease grade, flowering stage and dry weight per plant were measured and genetic diversity analysis of conserved DNA-derived polymorphism and sequence related amplified polymorphism was made by using 17 samples of M₃ generation Stylosanthes spp. seeds that were mutagenized by ⁶⁰Coγ-ray radiation and selected with excellent traits after 2 consecutive generations of single plant selection from M₂ generations. The genetic similarity coefficients of 17 Stylosanthes spp. were calculated using NTSYS-pc 2.1 software, as a result, 92 and 177 bands were amplified by 17 CDDP primers and 26 pairs of SRAP primers respectively, in which the number of polymorphic bands were 66 and 111 respectively, the polymorphism rates were 71.7% and 62.7%, the specific bands were 13 and 29, and the specificity rates were 14.1% and 16.4% respectively. The genetic similarity coefficient (GS) between germplasms ranged from 0.54~0.90 and 0.60~0.94, and the average genetic similarity coefficient were 0.76 and 0.79 respectively. The cluster map of 17 Stylosanthes spp. showed the 17 Stylosanthes spp. could be divided into 7 groups and the clustering results were basically consistent with the statistical results of agronomic traits when the genetic similarity coefficient was 0.82 according to the unweighted paired arithmetic average method and made based on the combination of CDDP and SRAP markers. This study provides a certain reference basis for the breeding of Stylosanthes spp. by radiation mutation in the future.

Key words: Stylosanthes spp., ⁶⁰Coγ-ray, Agronomic characters, CDDP, SRAP, Genetic diversity

中图分类号:

S335.21

罗铭欣, 刘凤民, 陈纪言, 崔均涛, 张伟丽. ⁶⁰Coγ辐射柱花草M₃代的农艺性状及遗传多样性分析[J]. 草地学报, 2021, 29(9): 1992-2000.

LUO Ming-xin, LIU Feng-min, CHEN Ji-yan, CUI Jun-tao, ZHANG Wei-li. Agronomic Traits and Genetic Diversity Analysis of M₃ Generation of Stylosanthes spp. Irradiated by ⁶⁰Coγ[J]. Acta Agrestia Sinica, 2021, 29(9): 1992-2000.

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参考文献

[1] 唐燕琼, 胡新文, 郭建春, 等. 柱花草种质遗传多样性的ISSR分析[J]. 草业学报, 2009, 18(1):57-64
[2] 张伟丽, 刘凤民, 刘艾.柱花草SRAP-PCR体系优化及其遗传多样性分析[J]. 草业学报, 2011, 20(4):159-168
[3] 丁泽婷, 尹晓畅, 唐燕琼, 等. 柱花草重要性状与SSR,SRAP分子标记的关联分析[J]. 分子植物育种, 2017, 15(5):1839-1845
[4] 唐燕琼, 胡新文, 郭建春, 等. 柱花草种质遗传多样性的ISSR分析[J]. 草业学报, 2009, 18(1):57-64
[5] 陈敦萍, 李凌, 沈世华, 等. 小桐子SRAP-PCR体系优化与M₁代变异植株的分子鉴定[J]. 核农学报, 2009, 23(2):209-213
[6] 李凤涛, 赵丽丽, 王普昶, 等. 辐射白刺花遗传多样性的ISSR和RAPD比较分析[J]. 草地学报, 2015, 23(5):1114-1118
[7] 罗铭欣, 刘凤民, 张伟丽, 等. SRAP和CDDP标记在巴戟天遗传多样性分析中的应用[J]. 分子植物育种, 2021,19(11):3661-3669
[8] 张宇, 张继, 荣涛, 等. 杧果品种亲缘关系的CDDP分析[J]. 浙江农业学报, 2020, 32(5):824-830
[9] 辛可慧, 文亦芾. 分子技术在柱花草上的应用研究进展[J]. 安徽农业科学, 2012, 40(34):16531-16532, 16540
[10] 黄春琼, 刘国道, 白昌军. 利用SRAP技术鉴定12个柱花草品种[J]. 分子植物育种, 2017, 15(7):2856-2862
[11] 卞华, 申晴, 韦海燕, 等. 基于SSR标记的柱花草种质资源遗传多样性与群体结构分析[J]. 草业科学, 2019, 36(3):729-743
[12] 尹晓畅, 唐燕琼, 李豆豆, 等. 柱花草种质遗传多样性的SSR和SRAP分析[J]. 热带作物学报, 2014, 35(7):1362-1367
[13] 蒋昌顺, 葛琴雅, 邹冬梅, 等. 柱花草RAPD反应体系的建立及其8个品种遗传多样性分析[J]. 广西植物, 2004, 24(3):243-247
[14] EVERLEY J G, AMANDA C M G, RICHARD J A. Use of the polymerase chain reaction to investigate the delimitation of two agronomically important species of Stylosanthes (Aubl.) Sw.[J]. Botanical Journal of Scotland, 1994, 47(1):83-96
[15] KAZAN K, MANNERS J M, CAMERON D F. Genetic variation in agronomically important species of Stylosanthes determined using random amplified polymorphic DNA markers[J]. Theoretical and Applied Genetics, 1993, 85(6-7):882-888
[16] 白昌军, 刘国道, 姚庆群, 等. 太空搭载‘热研2号’柱花草后代RAPD多态性分析[J]. 草地学报, 2008(4):336-340
[17] JIANG C S, MA X R, ZHOU D M, et al. AFLP analysis of genetic variability among Stylosanthes guianensis accessions resistant and susceptible to the stylo anthracnose[J]. Plant Breeding, 2005, 124(6):595-598
[18] CAMERON D F, MANNERS J M, CURTIS M D. Molecular evidence that diploid Stylosanthes humilis and diploid Stylosanthes hamata are progenitors of allotetraploid Stylosanthes hamata cv. Verano[J]. Genome, 1995, 38(2):344-348
[19] LIU C J, MUSIAL J M, THOMAS B D. Genetic relationships among Stylosanthes species revealed by RFLP and STS analyses[J]. TAG Theoretical and Applied Genetics, 1999, 99(7):1179-1186
[20] 张伟丽, 刘凤民. ⁶⁰Coγ辐照柱花草M₁代表型及分子水平诱变效果分析[J]. 草地学报, 2012, 20(3):505-511
[21] 刘凤民, 张伟丽, 陈梓健, 等. 辐照柱花草M2代的农艺性状及分子水平的诱变效果分析[J]. 广东农业科学, 2015, 42(4):28-34
[22] LI G, QUIROS C F. Sequence-related amplified polymorphism (SRAP), a new marker system based on a simple PCR reaction:its application to mapping and gene tagging in Brassica[J]. TAG Theoretical and Applied Genetics, 2001, 103(2):455-461
[23] COLLARD B C Y, MACKILL D J. Conserved DNA-Derived Polymorphism (CDDP):A Simple and Novel Method for Generating DNA Markers in Plants[J]. Plant Molecular Biology Reporter, 2009, 27(4):558-562
[24] CHAKRABORTY S. Expression of quantitative resistance to Colletotrichum gloeosporioides in Stylosanthes scabra at different inoculum concentrations and day-night temperatures[J]. Australian Journal of Agricultural Research, 1990, 41(1):89-100
[25] 肖鑫辉, 李开绵, 许瑞丽, 等. 国内外栽培木薯(Manihot esculenta Crantz)种质资源表型多样性分析[J]. 植物遗传资源学报, 2017, 18(1):94-105
[26] 任羽, 潘丙成, 陆顺教, 等. ⁶⁰Coγ辐射石斛兰组培苗的SRAP分析[J]. 中国农学通报, 2016, 32(31):85-89
[27] NEI M, LI W H. Mathematical model for studying genetic variation in terms of restriction endonucleases[J]. Proc Natl Acad Sci U S A, 1979, 76(10):5269-5273
[28] 张加强, 陈常理, 骆霞虹, 等. 26份黄麻种质资源产量性状的主成分聚类分析及其评价[J]. 植物遗传资源学报, 2016, 17(3):475-482

⁶⁰Coγ辐射柱花草M₃代的农艺性状及遗传多样性分析

Agronomic Traits and Genetic Diversity Analysis of M₃ Generation of Stylosanthes spp. Irradiated by ⁶⁰Coγ

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