北京西北浅山区退化草地植被恢复对土壤微生物群落多样性的影响

doi:10.11733/j.issn.1007-0435.2022.06.005

草地学报 ›› 2022, Vol. 30 ›› Issue (6): 1350-1358.DOI: 10.11733/j.issn.1007-0435.2022.06.005

• 生态与草原修复 • 上一篇

北京西北浅山区退化草地植被恢复对土壤微生物群落多样性的影响

阚海明, 庞卓, 陈超, 邹俊亮, 张国芳, 武菊英

北京草业与环境研究发展中心, 北京 100097

收稿日期:2021-10-20 修回日期:2022-01-02 发布日期:2022-07-05
通讯作者: 阚海明,E-mail:kanhaiming@hotmail.com;武菊英,E-mail:wujuying@grass-env.com
作者简介:阚海明(1984-),男,汉族,助理研究员,博士,主要从事草地生态学研究,E-mail:kanhaiming@hotmail.com
基金资助:
北京市农林科学院科技创新能力建设专项（KJCX20170301；KJCX20190404）；北京市自然科学基金（5204031）资助

Changes in Soil Microbial Communities Following the Vegetation Restoration of Degraded Sandy Grassland in Beijing

KAN Hai-ming, PANG Zhuo, CHEN Chao, ZOU Jun-liang, ZHANG Guo-fang, WU Ju-ying

Beijing Research and Development Center for Grass and Environment, Beijing 100097, China

Received:2021-10-20 Revised:2022-01-02 Published:2022-07-05

摘要/Abstract

摘要： 为了揭示退化草地生态系统恢复过程中土壤微生物群落对植被恢复的响应变化，本研究以补播建植6年的紫花苜蓿（Medicago sativa L.）草地（AF）和无芒雀麦（Bromus inermis Leyss.）草地（SB）为研究对象，采用高通量测序的方法分析土壤微生物细菌和真菌（群落多样性）在不同草地群落中的特征，结合地上植物多样性和土壤理化性质的变化，研究植被恢复对土壤微生物群落多样性的影响。结果表明：无芒雀麦和紫花苜蓿草地的土壤微生物量碳含量和微生物量氮含量要显著高于自然演替地（P<0.01）；2种人工草地（AF，SB）中土壤真菌和细菌的OTU数量显著地高于自然演替地（CK）（P<0.01）；不同植被恢复群落间的土壤细菌和真菌群落的β多样性差异显著。本研究结果表明利用紫花苜蓿和无芒雀麦进行植被恢复均对土壤微生物群落产生了显著的影响，且不同植被类型对土壤细菌和真菌的群落结构影响不同。

关键词: 植被恢复, 微生物多样性, 退化草地, 紫花苜蓿, 无芒雀麦

Abstract: To explore the soil microbial community changing mechanism following the vegetation restoration,a long-term restoration experiment was established (since 2014) on degraded sandy grassland to address the soil improvement progress. Bromus inermis (SB) and Medicago sativa (AF) were used as reseeding restoration methods,and a natural recovery site was used as a control. All soil microbial communities were characterized by using the Illumina MiSeq standard protocol for DNA sequencing. Soil bacterial and fungal abundance and diversity were calculated based on OTUs. Our results showed that both soil microbial biomass C and N in revegetation plots increased significantly compared to the natural recovery treat (P<0.05). The OTUs of AF and SB were significantly higher than that of CK since the very second year of the restoration. The β diversity was significantly different among different plant communities. The plant species diversity index of SB was significantly lower than that of CK and AF (P<0.01),however,there was no distinct similar difference in the diversity of microbial communities was observed,this suggests that the decreased plant diversity caused by one increasing dominant species such as smooth brome could not be consistent with the belowground communities. In contrast,different dominant plant species (SB,AF) could alter the relative abundances of specific bacteria and fungi at phylum level in this research.

Key words: Vegetation restoration, Microbial community diversity, Degraded grassland, Alfalfa, Smooth brome

中图分类号:

S154.36

阚海明, 庞卓, 陈超, 邹俊亮, 张国芳, 武菊英. 北京西北浅山区退化草地植被恢复对土壤微生物群落多样性的影响[J]. 草地学报, 2022, 30(6): 1350-1358.

KAN Hai-ming, PANG Zhuo, CHEN Chao, ZOU Jun-liang, ZHANG Guo-fang, WU Ju-ying. Changes in Soil Microbial Communities Following the Vegetation Restoration of Degraded Sandy Grassland in Beijing[J]. Acta Agrestia Sinica, 2022, 30(6): 1350-1358.

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北京西北浅山区退化草地植被恢复对土壤微生物群落多样性的影响

Changes in Soil Microbial Communities Following the Vegetation Restoration of Degraded Sandy Grassland in Beijing

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