柱花草响应炭疽菌侵染的差异磷酸化蛋白质组学分析

doi:10.11733/j.issn.1007-0435.2023.03.009

草地学报 ›› 2023, Vol. 31 ›› Issue (3): 699-709.DOI: 10.11733/j.issn.1007-0435.2023.03.009

柱花草响应炭疽菌侵染的差异磷酸化蛋白质组学分析

张世子, 杨丽云, 高静, 王芳, 蒋凌雁

海南大学热带作物学院/海南省热带生物资源可持续利用重点实验室, 海南海口 570228

收稿日期:2022-10-10 修回日期:2022-11-28 出版日期:2023-03-15 发布日期:2023-04-01
通讯作者: 蒋凌雁,E-mail:lyjiang@hainanu.edu.cn
作者简介:张世子(1997-),男,汉族,河南新乡人,硕士研究生,主要从事牧草与微生物互作方面的研究,E-mail:88047512@163.com
基金资助:
国家自然科学基金项目（31960342）；中国科协青年人才托举工程第六届项目（2020QNRC001）；海南省科协青年科技英才创新计划项目（QCXM202001）资助

Differential Phosphoproteomic Analysis of Stylosanthes in Response to Colletotrichum gloeosporioides

ZHANG Shi-zi, YANG Li-yun, GAO Jing, WANG Fang, JIANG Ling-yan

College of Tropical Crops, Hainan University/Key Laboratory of Sustainable Utilization of Tropical Biological Resources of Hainan Province, Haikou, Hainan Province 570228, China

Received:2022-10-10 Revised:2022-11-28 Online:2023-03-15 Published:2023-04-01

摘要/Abstract

摘要： 磷酸化是重要的蛋白翻译后修饰，在调节植物免疫方面起重要作用，但目前对柱花草与炭疽菌互作的磷酸化知之甚少。本文以太空诱变柱花草抗病株系‘2001-84’和感病株系‘2001-71’为试验材料，通过非标记定量磷酸化蛋白质组学，分析了两种株系接种炭疽菌前后的差异磷酸化蛋白。结果表明：在‘2001-84’和‘2001-71’中分别有138个和106个蛋白的磷酸化丰度特异性响应炭疽菌侵染。蛋白功能与代谢通路富集分析发现两者具有明显的差异：‘2001-84’特异性磷酸化蛋白主要定位在质膜附近，参与胞内信号传导，并显著富集在MAPK级联信号通路、植物激素信号传导、病原菌互作途径等代谢通路；‘2001-71’则更多的定位于细胞器膜上，参与细胞器的分解，代谢通路只在剪接体途径显著富集。通过对‘2001-84’特异性磷酸化蛋白进一步分析，发掘了跟抗病相关的蛋白。本文研究可为后续解析柱花草抗炭疽病的分子机制和培育柱花草抗病品种提供参考。

关键词: 柱花草, 胶胞炭疽菌, 磷酸化蛋白质组学, 抗病蛋白

Abstract: Phosphorylation is an important post-translational modification of protein, which plays an important role in regulating plant immunity. However, little is known about the phosphorylation in the interaction between Stylosanthes spp. (stylo) and Colletotrichum gloeosporioides. In this study, label-free based phosphoproteomics technology was used to study the phosphoproteome change of disease-susceptible stylo line ‘2001-71’ and resistant line ‘2001-84’ infected with C. gloeosporioides, which were created by space flight mutation. It had been identified out 138 and 106 differentially phosphorylated proteins (DPPs) in ‘2001-84’ and ‘2001-71’ respectively in response to C. gloeosporioides infection specifically. By the analysis of protein function of DPPs and enrichment analysis of metabolic pathway, it was found out a significant difference existed between the resistant and susceptible lines. The specific DPPs in the line ‘2001-84’ were mainly located around the plasma membrane, involving into the intracellular signal transduction, and significantly enriched in the MAPK cascade signaling, plant hormone signaling and pathogen interaction pathway checked out by metabolic pathway analysis. The specific DPPs in the line ‘2001-71’ were mainly localized in organelle membrane, involving in organelle decomposition, and only significantly enriched in the spliceosome pathway. Further analysis on the specific DPPs in the line ‘2001-84’, some proteins relevant to disease resistance had been uncovered. This study could provide a basis for further analysis on the resistance mechanisms in stylo to the anthracnose and breeding resistant cultivars of stylo.

Key words: Stylosanthes, Colletotrichum gloeosporioides, Phosphoproteomics, Resistance protein

中图分类号:

Q946.1

张世子, 杨丽云, 高静, 王芳, 蒋凌雁. 柱花草响应炭疽菌侵染的差异磷酸化蛋白质组学分析[J]. 草地学报, 2023, 31(3): 699-709.

ZHANG Shi-zi, YANG Li-yun, GAO Jing, WANG Fang, JIANG Ling-yan. Differential Phosphoproteomic Analysis of Stylosanthes in Response to Colletotrichum gloeosporioides[J]. Acta Agrestia Sinica, 2023, 31(3): 699-709.

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参考文献

[1] 邹晓燕, 王林杰, 黄杰. 柱花草SgNramp1和SgNramp2基因克隆与表达分析[J]. 草地学报, 2022, 30(6):1388-1395
[2] 王俐媛, 王坚, 丁西朋. 3种圭亚那柱花草根系分泌物对杂草幼苗生长的影响[J]. 草地学报, 2022, 30(6):1500-1508
[3] 严琳玲, 白昌军, 刘国道. 抗炭疽病柱花草空间育种新品系的多目标决策评价筛选[J]. 草地学报, 2009, 17(1):93-100, 105
[4] 易克贤. 柱花草炭疽病及其抗病育种进展[J]. 中国草地, 2001, 23(4):59-65
[5] WANG H, CHEN Z, LIU G, et al. Alterations of growth, antioxidant system and gene expression in Stylosanthes guianensis during Colletotrichum gloeosporioides infection[J]. Plant Physiol Biochem, 2017(118):256-266
[6] 付海天. 抗病与感病柱花草叶片结构及蛋白质比较研究[D]. 广州:华南热带农业大学, 2007:2-3
[7] 郑金龙, 李秋洁, 易克贤, 等. 9种杀菌剂对柱花草胶孢炭疽病菌的室内毒力测定[J]. 热带农业科学, 2015, 35(2):66-69
[8] ELMORE J M, GRIFFIN B D, WALLEY J W. Advances in functional proteomics to study plant-pathogen interactions[J]. Current Opinion in Plant Biology, 2021(63):102061
[9] ZULAWSKI M, BRAGINETS R, SCHULZE W X. PhosPhAt goes kinases-searchable protein kinase target information in the plant phosphorylation site database PhosPhAt[J]. Nucleic Acids Research, 2013, 41(Database issue):1176-1184
[10] ZHOU Q F, MENG Q, TAN X M, et al. Protein phosphorylation changes during systemic acquired resistance in Arabidopsis thaliana[J]. Frontiers in Plant Science, 2021(12):748287
[11] 陈阳, 高岩松, 李洪影, 等. 草地早熟禾蛋白激酶OSK4基因的克隆及对非生物胁迫响应分析[J]. 草地学报, 2022, 30(2):339-347
[12] NOUJAIM J, PAYNE L S, JUDSON I, et al. Phosphoproteomics in translational research:a sarcoma perspective[J]. Annals of Oncology, 2016, 27(5):787-794
[13] YU J J, GONZALEZ J M, DONG Z P, et al. Integrative proteomic and phosphoproteomic analyses of pattern- and effector-triggered immunity in tomato[J]. Frontiers in Plant Science, 2021(12):768693
[14] LI Y, YE Z, NIE Y, et al. Comparative phosphoproteome analysis of Magnaporthe oryzae-responsive proteins in susceptible and resistant rice cultivars[J]. Journal of Proteomics, 2015, 115(2):66-80
[15] 白昌军, 姚庆群, 刘国道. 空间辐射育种技术选育柱花草抗炭疽病新品系[J]. 热带作物学报, 2009, 30(8):1168-1175
[16] GAO M, WAN M, YANG L, et al. Molecular and physiological characterization of Arabidopsis Colletotrichum gloeosporioides pathosystem[J]. Plant Pathology, 2021, 70(5):1168-1179
[17] XING T, LAROCHE A. Revealing plant defense signaling:getting more sophisticated with phosphoproteomics[J]. Plant Signaling and Behavior,2011, 6(10):1469-1474
[18] COUTO D, ZIPFEL C. Regulation of pattern recognition receptor signaling in plants[J]. Nature Reviews Immunology, 2016, 16(9):537-552
[19] 杨茂霞, 林国彪, 陈彩虹, 等. 胶孢炭疽菌侵染柱花草叶片的显微观察[J].草业学报, 2015, 24(5):175-181
[20] 张晓林, 张俊娥, 贺璞慧中, 等. 胶孢炭疽菌侵染杨树叶片的组织病理学研究[J].北京林业大学学报, 2018, 40(3):101-109
[21] SUN T J, NITTA Y, ZHANG Q, et al. Antagonistic interactions between two MAP kinase cascades in plant development and immune signaling[J]. EMBO Reports, 2018, 19(7):e45324.
[22] ZHANG M M, SU J B, ZHANG Y, et al. Conveying endogenous and exogenous signals:MAPK cascades in plant growth and defense[J]. Current Opinion in Plant Biology, 2018, 45(10):1-10
[23] ASANO T, NGUYEN T H, YASUDA M, et al. Arabidopsis MAPKKK δ-1 is required for full immunity against bacterial and fungal infection[J]. Journal of Experimental Botany, 2020, 71(6):2085-2097
[24] SU J B, ZHANG M M, ZHANG L, et al. Regulation of stomatal immunity by interdependent functions of a pathogen-responsive MPK3/MPK6 Cascade and abscisic acid[J]. Plant Cell, 2017, 29(3):526-542
[25] FU M, BAI Q, ZHANG H, et al. Transcriptome analysis of the molecular patterns of pear plants infected by two Colletotrichum fructicola pathogenic strains causing contrasting sets of leaf symptoms[J]. Frontiers in Plant Science,2022(13):761133
[26] YANG Y X, AHAMMED G J, WU C J, et al. Crosstalk among jasmonate, salicylate and ethylene signaling pathways in plant disease and immune responses[J]. Current Protein and Peptide Science, 2015, 16(5):450-461
[27] YANG Y L, LI H G, LIU M Y, et al. PeTGA1 enhances disease resistance against Colletotrichum gloeosporioides through directly regulating PeSARD1 in poplar[J]. International Journal of Biological Macromolecules, 2022(214):672-684
[28] SUN X, LI A, MA G, et al. Transcriptome analysis provides insights into the bases of salicylic acid-induced resistance to anthracnose in sorghum[J]. Plant Molecular Biology,2022, 110(1-2):69-80
[29] LI N, HAN X, FENG D, et al. Signaling crosstalk between salicylic acid and ethylene/jasmonate in plant defense:do we understand what they are whispering?[J]. International Journal of Biological Science, 2019, 20(3):671
[30] ALVAREZ-DIAZ J C, LAUGÉ R, DELANNOY E, et al. Genome-wide transcriptomic analysis of the effects of infection with the hemibiotrophic fungus Colletotrichum lindemuthianum on common bean[J]. Plants-Basel, 2022, 11(15):1995
[31] JIANG C J, SHIMONO M, SUGANO S, et al. Abscisic acid interacts antagonistically with salicylic acid signaling pathway in rice-Magnaporthe grisea interaction[J]. Molecular Plant-Microbe Interactions, 2010, 23(6):791-798
[32] ADIE B A, PÉREZ-PÉREZ J, PÉREZ-PÉREZ M M, et al. ABA is an essential signal for plant resistance to pathogens affecting JA biosynthesis and the activation of defenses in Arabidopsis[J]. Plant Cell, 2007, 19(5):1665-1681
[33] KUMAR A S, LAKSHMANAN V, CAPLAN J L, et al. Rhizobacteria Bacillus subtilis restricts foliar pathogen entry through stomata[J]. Plant Journal, 2012, 72(4):694-706
[34] JETTER R, KUNST L, SAMUELS A L. Composition of plant cuticular waxes[M]. New Jersey:Blackwell Publishing Ltd, 2006:145-181
[35] KHANAL B P, KNOCHE M. Mechanical properties of cuticles and their primary determinants[J]. Journal of Experimental Botany, 2017, 68(19):5351-5367
[36] 张启辉, 李晓曼, 龙希洋, 等. 植物角质蜡质代谢及抗病机制研究[J]. 浙江农林大学学报, 2020, 37(6):1207-1215

柱花草响应炭疽菌侵染的差异磷酸化蛋白质组学分析

Differential Phosphoproteomic Analysis of Stylosanthes in Response to Colletotrichum gloeosporioides

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