草地学报 ›› 2026, Vol. 34 ›› Issue (10): 3795-3804.DOI: 10.11733/j.issn.1007-0435.2026.10.025

• 研究论文 • 上一篇    下一篇

干旱胁迫下三十烷醇对紫花苜蓿幼苗生长及生理特性的影响

贠海持, 南丽丽, 杜亮亮, 姚宇恒, 何圆圆   

  1. 甘肃农业大学草业学院/草业生态系统教育部重点实验室/农业农村部饲草种质创新与新品种选育重点实验室, 甘肃 兰州 730070
  • 收稿日期:2025-11-27 修回日期:2026-03-02 出版日期:2026-10-15 发布日期:2026-10-10
  • 通讯作者: 南丽丽, E-mail: nanll@gsau.edu.cn
  • 作者简介:贠海持(2002-),男,汉族,甘肃兰州人,硕士研究生,主要从事牧草栽培与育种研究,E-mail:1828612551@qq.com
  • 基金资助:
    财政部和农业农村部:国家现代农业产业技术体系(CARS-34)资助

The Effect of Triacontanol on Seedling Growth and Physiological Characteristics of Alfalfa under Drought Stress

YUN Hai-chi, NAN Li-li, DU Liang-liang, YAO Yu-heng, HE Yuan-yuan   

  1. College of Pratacultural Science, Gansu Agricultural University/Key Laboratory of Grassland Ecosystem, Ministry of Education/Key Laboratory of Forage Germplasm Innovation and New Variety Breeding of Ministry of Agriculture and Rural Affairs, Lanzhou, Gansu Province 730070, China
  • Received:2025-11-27 Revised:2026-03-02 Online:2026-10-15 Published:2026-10-10

摘要: 为探究干旱胁迫下外施三十烷醇(Triacontanol,TA)对紫花苜蓿(Medicago sativa)幼苗生长的缓解效应,本研究采用营养液砂培法,以抗旱新品系(P1、P2)和干旱敏感性材料(7809、15578)为研究对象,设置对照(CK)、干旱胁迫(-1.6MPa PEG-6000,T1)及干旱胁迫下喷施3,6,9,12 μmol·L-1 TA(分别记为T2,T3,T4,T5),测定各处理下供试紫花苜蓿的形态、生理及光合指标变化,分析不同浓度TA处理对紫花苜蓿幼苗生理特征的影响。结果表明:外施不同浓度的TA均对紫花苜蓿干旱胁迫有缓解效应,均能显著提高其株高、叶面积、根系活力、地上生物量、地下生物量及光合参数,显著降低可溶性糖、可溶性蛋白、脯氨酸、丙二醛和超氧阴离子含量。隶属函数分析表明,外源TA对紫花苜蓿干旱胁迫缓解能力由高到低的排序为:T3>T4>T2>T5>T1,材料间表现为15578>P1>7809>P2,其研究结果为TA在苜蓿耐旱栽培中的科学应用提供了实践指导。

关键词: 紫花苜蓿, 干旱胁迫, 三十烷醇, 光合生理

Abstract: To investigate the mitigating effects of exogenous triacontanol (TA) application on the growth of alfalfa (Medicago sativa) seedlings under drought stress, a sand culture method with nutrient solution was used. Drought-resistant new lines (P1, P2) and drought-sensitive materials (7809, 15578) were subjected to the following treatments: control (CK), drought stress (-1.6 MPa PEG-6000, T1), and drought stress combined with foliar sprays of TA at 3, 6, 9, and 12 μmol·L-1 (denoted as T2, T3, T4, and T5, respectively). Morphological, physiological, and photosynthetic parameters were measured to analyze the impact of different TA concentrations. The results indicated that all TA concentrations significantly alleviated drought stress by enhancing plant height, leaf area, root activity, above-ground biomass, below-ground biomass, and photosynthetic parameters, while significantly reducing contents of soluble sugar, soluble protein, proline, malondialdehyde, and superoxide anion. Membership function analysis ranked the mitigation efficacy of TA treatments as T3>T4>T2>T5>T1, and among materials as 15578>P1>7809>P2, demonstrating a greater alleviative effect on sensitive materials in general. These findings provide practical guidance for the scientific application of TA in drought-tolerant alfalfa cultivation.

Key words: Alfalfa, Drought stress, Triacontanol, Photosynthetic physiology

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