›› 2006, Vol. 14 ›› Issue (4): 319-323.DOI: 10.11733/j.issn.1007-0435.2006.04.005

• 研究论文 • 上一篇    下一篇

NaOH处理结缕草种子对种带真菌的影响

杨颜霞, 韩建国, 孙彦, 张颖, 秦歌菊   

  1. 中国农业大学草地研究所, 北京市草业科学重点实验室, 北京, 100094
  • 收稿日期:2006-03-06 修回日期:2006-06-06 出版日期:2006-11-15 发布日期:2006-11-15
  • 通讯作者: 韩建国,E-m ail:gras slab@pub lic3.bt a.net.cn
  • 作者简介:杨颜霞(1979- ),女,硕士研究生,主要从事种子病理学研究,E-mail:sunny2034@sohu.com
  • 基金资助:
    北京市重点实验室共建项目(JD100190531)

Effect of Sodium Hydroxide Treatment on Seed-borne Fungi of Zoysia japonica

YANG Yan-xia, HAN Jian-guo, SUN Yan, ZHANG Ying, QIN Ge-ju   

  1. Institute of Grassland Science, China Agriculture University, Key Laboratory of Grassland Science ofBeijing Municipality, Beijing 100094, China
  • Received:2006-03-06 Revised:2006-06-06 Online:2006-11-15 Published:2006-11-15

摘要: 用20%NaOH处理2002、2003和2004年收获的结缕草(Zoysia japonica)种子,采用PDA培养法测定种子带菌率、种带真菌的种类和分离频率。结果表明:NaOH处理结缕草种子降低其带菌率,与表面消毒效果相当,处理后随着贮藏时间的延长,种子带菌率升高。经过NaOH处理的种子再进行表面消毒效果不明显。在供试材料中共检出25属34种真菌,其中曲霉、青霉的分离频率最高。用NaOH浸泡和表面消毒种子均可以降低其带菌种类,且二者效果大致相同。

关键词: 结缕草, 种带真菌, NaOH, 带菌率, 带菌种类, 分离频率

Abstract: We studied the seed-borne fungi of Zoysia japonica for safe seed treatment and storage. Zoysia japonica seeds harvested in 2002, 2003, and 2004 were treated with 20% sodium hydroxide solution. To detect seed-borne fungi on/in the seed samples, PDA plate method was used. The results show that soaked in 20% sodium hydroxide solution, the seed reduced in the percentage of infection of seed-borne fungi; the effect was similar to seed surface sterilization. However, with prolonged storage time, the percentage of seed-borne fungi rose. Surface sterilization of previously sodium hydroxide treated Zoysia japonica seeds proved to be of no marked effect. On/in the samples, 34 fungi species of 25 genera had been identified. Among them, Penicillium spp. and Aspergillus spp. were the more frequently distinguished. Seed soaked in 20% sodium hydroxide solution or undergone surface sterilization attain to similar effect of reducing percentage of fungi infection and of fungi species. The two methods achieve similar results.

Key words: Zoysia japonica, Seed-borne fungi, Sodium hydroxide, Percentage of infected seed, Fungal species, Percentage incidence of fungi isolated

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