Research on Salt-Tolerance of Wheatgrass Transformed by P5CS Gene

doi:10.11733/j.issn.1007-0435.2006.01.005

›› 2006, Vol. 14 ›› Issue (1): 20-23.DOI: 10.11733/j.issn.1007-0435.2006.01.005

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Research on Salt-Tolerance of Wheatgrass Transformed by P5CS Gene

XU Chun-bo¹, MI Fu-gui², WANG-Yong²

1. Grassland Research Institute of Chinese Academy of Agriculture Sciences, Huhhot, Inner Mongolia Autonomous Region 010010, China;
2. Inner Mongolia Agriculture University, Huhhot, Inner Mongolia Autonomous Region 010019, China

Received:2005-03-15 Revised:2005-09-23 Online:2006-02-15 Published:2006-02-15

转基因冰草植株耐盐性研究

徐春波¹, 米福贵², 王勇²

1. 中国农业科学院草原研究所, 呼和浩特, 010010;
2. 内蒙古农业大学, 呼和浩特, 010019

通讯作者: 米福贵,E-mail:mfgui@yahoo.com.cn
作者简介:徐春波(1979- ),女,内蒙古扎兰屯市人,硕士,从事牧草育种工作,E-mail:xcb972002@163.com;
基金资助:
国家转基因植物研究与产业化专项资助(J-2002-B-008)

Abstract

Abstract: Plants transformed by P5CS gene(97#、19#、66#) and negatives were tested using several salt tolerance index such as proline content,leaf-water content,plasma membrane permeability and ratio of K⁺/Na⁺.The results show that the transgenic wheatgrass plants accumulated more proline than CK under salt-stress conditions,which proved that P5CSF129A had functioned in transgenic wheatgrass plants and had increased their salt-tolerance.The results also show that transgenic wheatgrass plants had nurtured greater salinity tolerance than CK.According to physiological target in synthetically evaluation,the result is 97#>19#>66#>Negatives.

Key words: Wheatgrass, Salt tolerance, P5CSF129A gene

摘要： 以转P5CSF129A基因的冰草属(Agropyron Gaertn)阳性植株(97#、19#、66#)和阴性植株为材料,测定叶片脯氨酸含量、相对含水量、质膜透性和K⁺/Na⁺比值4项耐盐指标。结果表明:转基因植株的脯氨酸含量明显高于阴性植株,说明P5CSF129A基因已经在冰草转化植株体内表达,提高了转基因植株的耐盐性;其它耐盐指标的结果也证明转基因冰草植株的耐盐性高于对照植株。对转基因植株和阴性植株的耐盐能力综合评定结果是97#>19#>66#>阴性植株。

关键词: 冰草, 耐盐指标, P5CSF129A基因

CLC Number:

Q943

XU Chun-bo, MI Fu-gui, WANG-Yong. Research on Salt-Tolerance of Wheatgrass Transformed by P5CS Gene[J]. , 2006, 14(1): 20-23.

徐春波, 米福贵, 王勇. 转基因冰草植株耐盐性研究[J]. , 2006, 14(1): 20-23.

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Research on Salt-Tolerance of Wheatgrass Transformed by P5CS Gene

转基因冰草植株耐盐性研究

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