Effects of Calcium and Calmodulin Antagonist W7 on Antioxidant Systems of Roots of Alfalfa Roots under PEG Stress

doi:10.11733/j.issn.1007-0435.2012.06.014

Acta Agrestia Sinica ›› 2012, Vol. 20 ›› Issue (6): 1072-1076.DOI: 10.11733/j.issn.1007-0435.2012.06.014

Previous Articles Next Articles

Effects of Calcium and Calmodulin Antagonist W7 on Antioxidant Systems of Roots of Alfalfa Roots under PEG Stress

ZHANG Chun-mei¹, XIE Xiao-rong¹, LIU Jin-rong^1,2, YAN Fang³

1. College of Agriculture and Biology Technology, Hexi University, Zhangye, Gansu Province 734000, China;
2. College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou, Gansu Province 730000, China;
3. Hexi Ecological & Oasis Agricultural Rsearch Institute, Zhangye, Gansu Province 734000, China

Received:2012-07-10 Revised:2012-09-24 Online:2012-12-15 Published:2012-12-28

Ca²⁺和钙调素拮抗剂W7对PEG胁迫下不同耐旱型紫花苜蓿抗氧化系统的影响

张春梅¹, 谢晓蓉¹, 刘金荣^1,2, 闫芳³

1. 河西学院农业与生物技术学院, 甘肃张掖 734000;
2. 兰州大学草地农业科技学院, 甘肃兰州 730000;
3. 河西生态与绿洲农业研究院, 甘肃张掖 734000

通讯作者: 谢晓蓉
作者简介:张春梅(1978-),女,甘肃酒泉人,博士,副教授,主要从事植物逆境生理研究,E-mail:zazcm197828@163.com;
基金资助:
甘肃省科技支撑项目(1011NKCG064)资助

Abstract

Abstract: The effect of Ca²⁺ and calmodulin antagonist W7 on antioxidant systems of alfalfa under drought stress induced by 10% polyethylene glycol (PEG6000) was studied using hydroponics culture. Results showed that alfalfa seedlings pretreated with calmodulin antagonist W7 under PEG stress had lower SOD and POD activities, and higher production rate of O^2-?, MDA and H₂O₂, and the aggravated damage of AsA and GSH. On the contrary, Ca²⁺ treatment enabled alfalfa to keep relatively higher activities of SOD and POD, lower production rate of O^2-穉nd MDA content, at the same time, alleviated the accumulation of AsA and GSH. These results indicated that Ca²⁺-CaM signal transduction might regulate the resistance of alfalfa to PEG stress by affecting the activity of some antioxidant enzymes and the content of antioxidant substance. The experiment showed that Ca²⁺- CaM signal system played an important regulating role in alleviating PEG stress.

Key words: Ca²⁺, Calmodulin antagonist W7, PEG stress, Alfalfa, Antioxidatant system

摘要： 以陇东苜蓿(抗旱型)、BL-02-329 (干旱敏感型) 2 种抗旱性强弱差异较大的紫花苜蓿(Medicago sativa L.)为试材,采用水培法研究Ca²⁺和钙调素拮抗剂W7预处理对干旱胁迫下紫花苜蓿根系抗氧化系统的影响。结果表明:钙调素拮抗剂W7[N-(6-aminohexyl)-5-chloro-1-naphthalene sulfonamide]浸种处理显著提高了不同耐旱型紫花苜蓿丙二醛(MDA)、H₂O₂含量和O^2-·产生速率,抑制了超氧化物歧化酶(SOD)和过氧化物酶(POD)活性,加剧了抗坏血酸(AsA)和还原型谷胱甘肽(GSH)的破坏;而Ca²⁺处理显著降低了PEG胁迫下紫花苜蓿MDA、H₂O₂ 含量和O^2-·产生速率,提高了SOD和POD活性,减轻了干旱胁迫对AsA和GSH的破坏。相同处理条件下,抗旱型品种陇东较干旱敏感型品种BL-02-329伤害程度轻,Ca²⁺-CaM信号系统可缓解干旱胁迫对紫花苜蓿的伤害。

关键词: Ca²⁺处理, 钙调素拮抗剂W7, 干旱胁迫, 紫花苜蓿, 抗氧化系统

CLC Number:

Q945.78

ZHANG Chun-mei, XIE Xiao-rong, LIU Jin-rong, YAN Fang. Effects of Calcium and Calmodulin Antagonist W7 on Antioxidant Systems of Roots of Alfalfa Roots under PEG Stress[J]. Acta Agrestia Sinica, 2012, 20(6): 1072-1076.

张春梅, 谢晓蓉, 刘金荣, 闫芳. Ca²⁺和钙调素拮抗剂W7对PEG胁迫下不同耐旱型紫花苜蓿抗氧化系统的影响[J]. 草地学报, 2012, 20(6): 1072-1076.

0
/ / Recommend

Add to citation manager EndNote|Ris|BibTeX

URL: https://manu40.magtech.com.cn/Jweb_cdxb/EN/10.11733/j.issn.1007-0435.2012.06.014

https://manu40.magtech.com.cn/Jweb_cdxb/EN/Y2012/V20/I6/1072

References

[1] Bohnert H J, Jensen R G. Strategies for engineering water stress tolerance in plants[J]. Trends in Biotechnology,1996,14(3):89-97
[2] Xiong L M, Schumaker K S, Zhu J K. Cell signaling during cold, drought, and salt stress[J]. The Plant Cell,2002,14(S):165-183
[3] 贾虎森,蔡世英,李德全,等. 土壤干旱胁迫下钙处理对芒果幼苗光合作用的影响[J].果树科学,2000,17(1):52-56
[4] Nayyar H, Kaushal S K. Chilling induced oxidative stress in germinating wheat grains as affected by water stress and calcium[J]. Biologia Plantarum,2002,45(4):601-604
[5] Gong M, vander Liut A, Knight M R, et al. Heat-shock induced changes in intracellular Ca²⁺ level in tobacco seedlings in relation to thermotolerance[J]. Plant Physiology,1998,116(1):429-437
[6] Gong M, Chen S N, Song Y Q, et al. Effect of calcium and calmodulin on intrinsic heat tolerance in relation to antioxidant systems in maize seedlings[J]. Australian Journal of Plant Physiology,1997,24(3):371-379
[7] 马淑英,赵明. 钙对拟南芥耐盐性的调节[J]. 作物学报,2006,32(11):1706-1711
[8] Bush D S. Regulation of cytosolic calcium in plants[J]. Plant Physiology,1993,103(1):7-9
[9] Hiroyoshi Hidaka, Yasuharu Sasaki, Toshio Tanaka, et al. N-(6-aminohexyl)-5-Chloro-l-naphthalenesulfonamide, a calmodulin antagonist, inhibits cell proliferation[J]. Proceedings of the National Academy of Sciences of USA,1981,78(7):4354-4357
[10] 梁颖,王三根. Ca²⁺ 对低温下水稻幼苗膜的保护作用[J]. 作物学报,2001,27(1):59-64
[11] 韩瑞宏,卢欣石,高桂娟,等. 紫花苜蓿抗旱性主成分及隶属函数分析[J]. 草地学报,2006,14(2):142-146
[12] 梁帅克,樊宏,张文明,等. 无机盐溶液引发对紫花苜蓿种子活力及幼苗抗逆性的影响[J]. 草原与草坪,2010,30(3):61-65
[13] Jiang M Y, Zhang J H. Effect of abscisic acid on active oxygen species, antioxidative defence system and oxidative damage in leaves of maize seedlings [J]. Plant Cell Physiology,2001,42(11):1265-1273
[14] Uchida A, Andre T I, Takashi H. Effects of hydrogen peroxide and nitric oxideon both salt and heat stress tolerance in rice[J]. Plant Science,2002,163(3):515-523
[15] Ma F W, Cheng L L. The sun-exposed peel of apple fruit has higher xanthophyll cycle-dependent thermal dissipation and antioxidants of the ascorbate-glutathione pathway than the shade peel[J]. Plant Science,2003,165(4):819-827
[16] Ma F W, Cheng L L. Exposure of the shaded side of apple fruit to full sun leads to up-regulation of both xanthophyll cycle and the ascorbate-glutathione cycle[J]. Plant Science,2004,166(6):1479-1486
[17] 龚吉蕊,赵爱芬,张立新,等. 干旱胁迫下几种荒漠化植物抗氧化能力的比较研究[J]. 西北植物学报,2004,24(9):1570-1577
[18] Sanders D, Brown I C, Harper J F. Communicating with calcium[J]. Plant Cell,1999,11(4):691-706
[19] Rudd J J, Frankling T V E. Unravelling response-specificity in Ca²⁺ signaling pathways in plant cells[J]. New Phytologist,2001,151(1):7-33
[20] Snedden W A, Fromm H. Calmodulin as a versatile calcium signal transducer in plants[J]. New Phytologist,2001,151(1):35-66
[21] 雷江丽,杜永臣,朱德蔚,等. 低温胁迫下不同耐冷性番茄品种幼叶细胞Ca²⁺分布变化的差异[J]. 园艺学报,2000,27(4):269-275
[22] 高洪波,陈贵林. 钙调素拮抗剂与钙对茄子幼苗抗冷性的影响[J]. 园艺学报,2002,29(3):243-246
[23] 陈贵林,贾开志. 钙和钙调素拮抗剂对高温胁迫下茄子幼苗抗氧化系统的影响[J]. 中国农业科学,2005,38(1):197-202
[24] 张宗申,利容千,王建波. 外源Ca²⁺预处理对高温胁迫下辣椒叶片细胞膜透性和GSH、AsA 含量及Ca²⁺分布的影响[J]. 植物生态学报,2001,25(2):230-234
[25] 郭丽红,陈善娜,龚明. 钙对玉米幼苗谷胱甘肽还原酶活性的影响[J]. 植物生理学通讯,2002,38(2):115-117

Effects of Calcium and Calmodulin Antagonist W7 on Antioxidant Systems of Roots of Alfalfa Roots under PEG Stress

Ca²⁺和钙调素拮抗剂W7对PEG胁迫下不同耐旱型紫花苜蓿抗氧化系统的影响

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	WANG Jing, LIU Xiao-jing, Cheng Tian-tian, Tong Chang-chun, WANG Xue. Study on Leaf Characteristics and Yield Effect of Alfalfa with Different Nitrogen Efficiency [J]. Acta Agrestia Sinica, 2021, 29(9): 1941-1949.
[2]	MENG Xuan-chen, MA Peng-cheng, MA Jie, DUAN Zhen, HAN Yang-yang, ZHANG Ji-yu. Study on the Effect of Mulching Furrow and Fertilization of Alfalfa in Semi-arid Areas of Loess Plateau [J]. Acta Agrestia Sinica, 2021, 29(9): 2098-2106.
[3]	CHEN Lei, BAO Xue-yan, GUO Gang, HUO Wen-jie, LI Qing-hong, XU Qing-fang, WANG Cong, LIU Qiang. Effects of Tannic Acid and Lactic Acid Bacteria on Alfalfa Silage Quality and Ruminal Fermentation in Vitro [J]. Acta Agrestia Sinica, 2021, 29(8): 1853-1858.
[4]	LI Chun-pin, QIU Yi-hui, XIA Hou-yin, TAN Lu-na, XU Huan-yu, ZHANG Han-wen, GAO Jing-hui. Membership Function Analysis of Low Transpiration and Yield Traits in Alfalfa Multi-paternal Hybrid F₁Generation [J]. Acta Agrestia Sinica, 2021, 29(7): 1416-1422.
[5]	LI Tian-qi, LIN Zhi-ling, LU Xuan, HUANG Jia-yuan, YANG Ning, WO Ye, GAO Kai, LIU Xiao-ni, WANG Xian-guo. Effects of Irrigation Amount on Soil Available Nutrient Distribution and Leaching Loss of Alfalfa in Horqin Sandy Land [J]. Acta Agrestia Sinica, 2021, 29(7): 1454-1461.
[6]	LI Hong, LI Bo, FANG Zhi-jian, YANG Zhao, ZHANG Chao, FENG Cheng-long, JIN Shan-shan, WANG Si-qi, SHI Wan-ying. Physiological Response of Alfalfa Seedlings to Freeze-thaw or Freeze-thaw Versus Soda Saline-alkali Stress [J]. Acta Agrestia Sinica, 2021, 29(7): 1469-1476.
[7]	LI Peng-zhen, ZHAO De-qin, DENG Bo, Yang Jun, Zhao Guo-hua, Zhao Liang. Effects of Biochar Addition and Drought Stress on Photosynthetic Efficiency and Growth of Alfalfa Inoculated with Rhizobia [J]. Acta Agrestia Sinica, 2021, 29(6): 1257-1264.
[8]	WANG Wen-jing, MA Dong-mei, ZHAO Li-juan, MA Qiao-li. Effects of 2,4-table Brassinolide on Enzyme Activity and Root ion Distribution and Absorption in Alfalfa Seedlings [J]. Acta Agrestia Sinica, 2021, 29(6): 1363-1368.
[9]	SUN Lei-lei, ZHAO Gui-qin, CHAI Ji-kuan, LIU Fu-yuan, Nie Xiumei, WANG Jun. Effects of Storage Methods and Time on Mould Quantity and Species in Alfalfa Hay [J]. Acta Agrestia Sinica, 2021, 29(5): 884-893.
[10]	HUANG Rong, YAO Bo, ZHANG Yu-juan, JIA Qian-ying, LI Xiang-yang, ZHANG Hong, ZHANG Zhen-fen. Effect of Pantoea agglomerans CQ10 Lipopolysaccharide on Seed Germination and Seedling Growth of Alfalfa [J]. Acta Agrestia Sinica, 2021, 29(5): 965-971.
[11]	CHEN Wei-dong, ZHANG Yu-xia, CONG Bai-ming, A Ma-er, TIAN Yong-lei, ZHANG Qing-xin, DU Xiao-yan. Effects of Potassium Fertilizer on MDA,SP Content and Antioxidant System of Alfalfa Root Neck [J]. Acta Agrestia Sinica, 2021, 29(4): 717-723.
[12]	XU Hong-yu, ZHEN Li-li, LI Yu-ying, DONG Kuan-hu, LI Xiang-lin. Effect of Freeze-drying Environment on Freezing Tolerance of Alfalfa Crowns [J]. Acta Agrestia Sinica, 2021, 29(4): 724-733.
[13]	LI Man-you, MA Zhong-ren, WANG Bing, YANG Yu-qi, SHEN Xiao-tian, CAO Li-juan, LI Xiao-yun, LAN Jian. Study on the Mixed Planting Mode of Two Alfalfa Varieties in Different Row Ratios in the Yellow River Irrigation Area in Ningxia [J]. Acta Agrestia Sinica, 2021, 29(4): 798-804.
[14]	HOU Hong-yan, SHEN Chen, HUO Wen-jie, LIU Qiang, ZHANG Shuan-lin, WANG Cong, CHEN Lei, XU Qing-fang, WANG Yong-xin, WANG Zhong-hao, GUO Gang. The Effects of Homofermentative Lactobacillus on Ensiling Characteristics and in Vitro Digestibility of Low-moisture Alfalfa Silage [J]. Acta Agrestia Sinica, 2021, 29(4): 835-841.
[15]	CHEN De-kui, GUO Xiang, ZHENG Ming-yang, CHEN Xiao-yang, ZHOU Wei, ZHANG Qing. The Effects of Amomum Villosum Lour. Essential Oil on the Fermentation Quality of Alfalfa [J]. Acta Agrestia Sinica, 2021, 29(4): 855-860.

Effects of Calcium and Calmodulin Antagonist W7 on Antioxidant Systems of Roots of Alfalfa Roots under PEG Stress

Ca2+和钙调素拮抗剂W7对PEG胁迫下不同耐旱型紫花苜蓿抗氧化系统的影响

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

Ca²⁺和钙调素拮抗剂W7对PEG胁迫下不同耐旱型紫花苜蓿抗氧化系统的影响