Preliminary Study on Drought Tolerance of Alfalfa Seedlings Induced by Piriformospora indica Fungi

doi:10.11733/j.issn.1007-0435.2013.06.029

›› 2013, Vol. 21 ›› Issue (6): 1218-1222.DOI: 10.11733/j.issn.1007-0435.2013.06.029

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Preliminary Study on Drought Tolerance of Alfalfa Seedlings Induced by Piriformospora indica Fungi

WU Mei-yan¹, HAO Ruo-chao¹, ZHANG Wen-ying¹, ZHANG Yue-xue², TANG Feng-lan²

1. College of Agriculture, Yangtze University, Jinzhou, Hubei Province 434025, China;
2. Institute of Pratacultural Sciences, Heilongjiang Academy of Agricultural Sciences, Harbin, Heilongjiang Province 150086, China

Received:2013-04-25 Revised:2013-05-09 Online:2013-12-15 Published:2013-11-27

印度梨形孢诱导紫花苜蓿提高抗旱性研究初报

武美燕¹, 蒿若超¹, 张文英¹, 张月学², 唐凤兰²

1. 长江大学农学院, 湖北荆州 434023;
2. 黑龙江省农业科学院草业研究所, 黑龙江哈尔滨 150086

通讯作者: 张文英
作者简介:武美燕（1977-），女，内蒙古呼和浩特人，博士，讲师，主要从事植物营养相关研究，E-mail：wumeiyan2002@163.com
基金资助:
国家公益性行业（农业）科研专项（201203032）；长江大学博士科研启动基金（801180010116）资助

Abstract

Abstract: The colonization of Piriformospora indica fungi in the roots of alfalfa seedlings was examined and the effects of the colonization on the growth and development of alfalfa seedling were investigated under drought stress by pot experiment. Results showed that Piriformospora indica could colonize the epidermal and root hair cells of alfalfa roots. The colonization of Piriformospora indica promoted root growth, aboveground dry matter accumulation and increased lateral root growth, chlorophyll content significantly under drought stress. These results indicated that Piriformospora indica infection could improve the drought resistance of alfalfa seedling and ensure the normal growth of alfalfa seedling.

Key words: Piriformospora indica, Afalfa, Seedling, Drought Resistance

摘要： 盆栽条件下研究了印度梨形孢（Piriformospora indica）在紫花苜蓿（Medicago sativa）幼苗上的定殖以及在干旱胁迫下对紫花苜蓿幼苗生长发育的影响。结果表明：印度梨形孢均能在紫花苜蓿根系中定殖。在干旱胁迫条件下，印度梨形孢真菌能够显著促进紫花苜蓿幼苗根系生长、侧根形成和地上部干物质积累（P<0.05），并增加了叶片叶绿素的含量。说明印度梨形孢真菌能够提高紫花苜蓿幼苗的抗旱能力，保证其在干旱胁迫条件下可以正常生长。

关键词: 印度梨形孢真菌, 苜蓿, 苗期, 抗旱性

CLC Number:

WU Mei-yan, HAO Ruo-chao, ZHANG Wen-ying, ZHANG Yue-xue, TANG Feng-lan. Preliminary Study on Drought Tolerance of Alfalfa Seedlings Induced by Piriformospora indica Fungi[J]. , 2013, 21(6): 1218-1222.

武美燕, 蒿若超, 张文英, 张月学, 唐凤兰. 印度梨形孢诱导紫花苜蓿提高抗旱性研究初报[J]. , 2013, 21(6): 1218-1222.

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References

[1] Verma S, Varma A, Rexer K H, et al. Piriformospora indica, gen. et sp. Nov., a new root-colonizing fungus[J]. Mycologia,1998,90(5):896-903
[2] Varma A, Verma S, Sudha A, et al. Piriformospora indica, acultivable plant growth promoting root endophyte[J]. Applied and Environmental Microbiology,1999,65(6):2741-2744
[3] Singh A, Sharma J, Rexer K H, et al. Plant productivity determinants beyond minerals, water and light: Piriformospora indica - A revolutionary plant growth promoting fungus[J]. Current Science,2000,79(11):1548-1554
[4] Rai M, Acharya D, Singh A, et al. Positive growth responses of the medicinal plants Spilanthes calva and Withania somnifera to inoculation by Piriformospora indica in a field trial[J]. Mycorrhiza,2001,11(3):123-128
[5] Waller F, Achatz B, Baltruschat H, et al. The endophytic fungus Piriformospora indica reprograms barley to salt-stress tolerance, disease resistance, and higher yield[J]. Proceedings of the National Academy of Science of United States of America,2005,102(38):13386-13391
[6] Sun C, Johnson J M, Cai D, et al. Piriformospora indica confers drought tolerance in Chinese cabbage leaves by stimulating antioxidant enzymes, the expression of drought-related genes and the plastid-localized CAS protein[J]. Journal of Plant Physiology,2010,167(12):1009-1017
[7] Sherameti I, Tripathi S, Varma A, et al. The root-colonizing endophyte Pirifomospora indica confers drought tolerance in Arabidopsis by stimulating the expression of drought stress-related genes in leaves[J]. Molecular Plant-Microbe Interactions,2008,21(6):799-807
[8] Hill T W, Kafer E. Improved protocols for Aspergillus minimal medium: Trace element and minimal medium salt stock solutions[J].Fungal Genetics Newsletter,2001,48:20-21
[9] 王凤让,毛克克,李国钧,等. 印度梨形孢及其近似种Sebacina vermifera促进番茄生长发育及磷吸收[J]. 浙江大学学报:农业与生命科学版,2011,37(1):61-68

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Preliminary Study on Drought Tolerance of Alfalfa Seedlings Induced by Piriformospora indica Fungi

印度梨形孢诱导紫花苜蓿提高抗旱性研究初报

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