Acta Agrestia Sinica ›› 2015, Vol. 23 ›› Issue (1): 14-20.DOI: 10.11733/j.issn.1007-0435.2015.01.003

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Research Progress of Forage Karyotype and Chromosome Banding Analyses

ZHENG Hui-min1, MAO Pei-sheng1, HUANG Ying1, CHEN Ling-ling1,2, YAN Hui-fang1, CHEN Quan-zhu1   

  1. 1. Department of Grassland Science, College of Animal Science and Technology, China Agricultural University Beijing Key Laboratory of Grassland Science, Beijing 100193, China;
    2. Chifeng Academy of Agricultural and Animal Sciences, Institute of Grassland Research, Chifeng, Inner Mongolia 024031, China
  • Received:2014-04-15 Revised:2014-08-23 Online:2015-02-15 Published:2015-01-31

牧草染色体核型和带型分析研究进展

郑慧敏1, 毛培胜1, 黄莺1, 陈玲玲1,2, 闫慧芳1, 陈泉竹1   

  1. 1. 中国农业大学动物科技学院草业科学系 草业科学北京市重点实验室, 北京 100193;
    2. 赤峰市农牧科学研究院草原研究所, 内蒙古 赤峰 024031
  • 通讯作者: 毛培胜
  • 作者简介:郑慧敏(1991-),女,山东菏泽人,硕士研究生,研究方向为牧草种子生理与良种扩繁,E-mail:zhenghuimin5210@163.com
  • 基金资助:

    国家"十二五"科技支撑计划课题(2011BAD17B01-02)资助

Abstract:

The karyotype and chromosome banding analyses of plants played an important role on the construction of genetic map, identification of genetic relationship, preservation of germplasm resources, exploration of evolution process, plant classification and breeding. This paper analyzed the similarities and differences between karyotype and chromosome banding researches systematically, and summarized the research progress about karyotype and chromosome banding analysis of forage plants from the aspects of sampling, pretreatment, stationary, dissociation, squash and dye. However, it is necessary for the analysis methods of karyotype and banding pattern to be further specified and standardized, and it will be the emphasis of karyotype and chromosome banding analysis on the evaluation of germplasm resources and the physiology research of seed deterioration in forage plants.

Key words: Forage, Karyotype, Chromosome banding, Research progress

摘要:

植物染色体的核型和带型分析对物种基因图谱的构建、亲缘关系的确定、种质资源的保存、进化过程的探索、植物学分类及育种等方面的研究具有重要作用.分析了植物染色体核型和带型研究的异同点,并从取材、预处理、固定、解离、制片及染色等方面系统综述了牧草核型和带型分析的研究进展.核型和带型分析方法需要进一步的规范和标准化,在种质资源评价、种子劣变生理等方面将成为牧草核型与带型分析研究的重点.

关键词: 牧草, 核型, 带型, 研究进展

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