Using Microwave Treatment to Construct Endophyte-free Melica transsilvanica Population

doi:10.11733/j.issn.1007-0435.2016.05.013

Acta Agrestia Sinica ›› 2016, Vol. 24 ›› Issue (5): 1016-1021.DOI: 10.11733/j.issn.1007-0435.2016.05.013

Previous Articles Next Articles

Using Microwave Treatment to Construct Endophyte-free Melica transsilvanica Population

SHI Chong, ZHANG Rui-si, HUANG Chang-fu, XING Yuan-tong, AN Sha-zhou

College of Grassland and Environmental Science, Xinjiang Agricultural University, Key Laboratory of Grassland Resources and Ecology of Xinjiang, Urumqi, Xinjiang 830052, China

Received:2015-06-30 Revised:2016-04-14 Online:2016-10-15 Published:2017-01-13

微波处理构建不感染内生真菌德兰臭草种群的方法探讨

施宠, 张蕊思, 黄长福, 邢圆通, 安沙舟

新疆农业大学草业与环境科学学院新疆草地资源与生态重点实验室, 新疆乌鲁木齐 830052

通讯作者: 安沙舟
作者简介:施宠(1979-),女,河北蠡县人,博士,讲师,研究方向为环境微生物,E-mail:shichong98@163.com
基金资助:
新疆农业大学草业科学国家重点学科博士研究生创新基金研究项目（XJCYB-2011-01）；教育部高等学校博士学科点专项科研基金联合资助课题（20126504110001）资助

Abstract

Abstract:

In this study mid-low microwave level treatment was used to construct endophyte-free populations of Melica transsilvanica. The elimination effects of the microwave on endophyte were tested by microscope test combined with Endophyte separation, seeds germination index and plant growth index. The result showed that 30 s microwave treatment was effective in killing the endophyte in the seeds. At the same time, there were no obvious adverse effects on the seeds germination rate, germinating potential and germination index. 10 s and 20 s treatment in microwave could promote plant growth and decrease endophyte infection rate by 10.7% and 25%, respectively. However, longer time treatment, 40 s and more treatment time could inhibit the growth of plant. Therefore, microwave method was an effective way to construct endophyte-free populations of Melica transsilvanica.

Key words: Endophyte, Microwave treatment, Melica transsilvanica, Seed germination index, Plant growth index

摘要：

微波技术会对生物组织产生一定的生物学效应。为了研究微波技术对德兰臭草（Melica transsilvanica）内生真菌的去除效果，采用微波中低档对感染内生真菌的德兰臭草种子进行不同时间的杀菌处理，通过显微镜检结合植株内生真菌分离技术、种子萌发指标、植株生长指标评价内生真菌去除效果。结果表明，输出功率300 W的微波处理30 s能有效杀灭种子中的内生真菌，并对种子发芽率、发芽势和发芽指数均无显著影响；微波处理10 s，20 s分别降低植株带菌率的10.7%，25%，并且对植株生长有促进作用；微波处理较长时间（40 s及以上时间）虽能有效杀灭种子中的内生真菌，但对种子萌发、植株生长有抑制作用。因此，微波处理种子技术是一种有效、简便、可行的内生真菌去除技术。

关键词: 内生真菌, 微波处理, 德兰臭草, 萌发指标, 生长指标

CLC Number:

S543.9

SHI Chong, ZHANG Rui-si, HUANG Chang-fu, XING Yuan-tong, AN Sha-zhou. Using Microwave Treatment to Construct Endophyte-free Melica transsilvanica Population[J]. Acta Agrestia Sinica, 2016, 24(5): 1016-1021.

施宠, 张蕊思, 黄长福, 邢圆通, 安沙舟. 微波处理构建不感染内生真菌德兰臭草种群的方法探讨[J]. 草地学报, 2016, 24(5): 1016-1021.

0
/ / Recommend

Add to citation manager EndNote|Ris|BibTeX

URL: https://manu40.magtech.com.cn/Jweb_cdxb/EN/10.11733/j.issn.1007-0435.2016.05.013

https://manu40.magtech.com.cn/Jweb_cdxb/EN/Y2016/V24/I5/1016

References

[1] Siegel M R, Latch G C M, Johnson M C. Fungal endophyte of grasses[J]. Annual Review of Phytopathology,1987(25):293-315
[2] 宋梅玲,李春杰,彭清青,等. 温度和水分胁迫下内生真菌对野大麦种子发芽的影响[J]. 草地学报,2010,18(6):833-837
[3] Latch G C M. Physiological interactions of endophyte fungi and their hosts[J]. Ecosystems and Environment,1993(44):143-156
[4] Clay K, Christopher S. Evolutionary origins and ecological consequences of endophyte symbiosis with grasses[J]. The American Naturalist,2002(16):99-127
[5] 南志标,李春杰. 禾草-内生真菌共生体在草地农业系统中的作用[J]. 生态学报,2004,24(3):605-616
[6] 崔乃然. 新疆植物志(第六卷)[M]. 乌鲁木齐:新疆科技卫生出版社(K):1996:54-55
[7] Siegel M R, Latch G C M, Johnson M C. Acremonium fungal endophytes of tall fescue and perennial ryegrass:Significance and control[J]. Plant Disease,1985,69(2):179-183
[8] Siegel M R, Latch G C M, Johnson M C. Fungal endophytes of grass[J]. Annual Review of Phytopathology,1987(25):293-315
[9] 傅荣兴,鲍正发,方欣. 微波处理对水稻种子生理的影响[J]. 种子,1994(6):68-69
[10] 李建忠. 微波对食品微生物的非热生物效应与微波杀菌技术[J]. 西南民族大学学报(自然科学版),2006,32(6):1219-1222
[11] 许志刚. 普通植物病理学(3版)[M]. 北京:中国农业出版社,2003:354-355
[12] Latch C, Christensen M. Artificial infection of grasses with endophytes[J]. Annals of Applied Biology,1985,107(1):17-24
[13] 施宠,王纯利,黄长福,等. 镉胁迫对野燕麦幼苗生长及其生理特性的影响[J]. 草地学报,2015,23(3):526-532
[14] 文卿琳,王兴鹏. 温度对棉花种子萌发的影响[J]. 安徽农业科学,2008,36(9):3513-3515
[15] 白小明,王靖婷,贺佳圆,等. 8个野生早熟禾种子萌发期耐盐性研究[J]. 草地学报,2013,21(3):546-555
[16] 段晓明,林秀华,刘传琴,等. 不同剂量微波处理对稻种发芽率和水稻产量的影响[J]. 湖南农业科学,2010(7):44-45
[17] 韩玉竹,李阳春,刘晓静,等. 微波处理对紫花苜蓿种子萌发特性的影响[J]. 种子,2010,29(10):70-71
[18] 张春香,王立坤,刘准. 微波辐射种子对黄瓜幼苗生长和根活力的影响[J]. 农业技术,1999,19(5):64-66
[19] 刘涛,李丽,曲海霞,等. 小麦网腥黑穗病菌微波处理技术研究[J]. 西南大学学报(自然科学版),2011,33(8):42-45
[20] Latch G C M, Christensen M J. Ryegrass endophyte incidence and control[J]. New Zealand Journal of Agriculture Research,1982,25(3):443-448
[21] 姚祥,李秀璋,朱小晓,等. 两种杀菌剂对中华羊茅种传内生真菌的影响[J]. 草业科学,2013,30(10):1517-1522
[22] Williams M J, Bac km an P A, Clark E M. Seed treatments for control of the tall fescue endophyte Acremonium coenophialum[J]. Plant Disease,1984(68):49-52

Using Microwave Treatment to Construct Endophyte-free Melica transsilvanica Population

微波处理构建不感染内生真菌德兰臭草种群的方法探讨

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 13

Recommended Articles

Metrics

Comments

[1]	LIAN He-na, CHEN Tai-xiang, CHEN Zhen-jiang, WEI Xue-kai, LI Chun-jie. Potential Analysis of Achnatherum inebrians Epichloё Endophyte Infestation as an Airfield Grass to Repel Birds [J]. Acta Agrestia Sinica, 2021, 29(8): 1649-1657.
[2]	ZHU Hai-xia, MA Yong-qiang. Development of the Wettable Powder of Fungal Endophyte HL-1 and Evaluation of Its Herbicidal Activity [J]. Acta Agrestia Sinica, 2019, 27(5): 1301-1308.
[3]	GUO Yan-e, Li Ying-de, GAO Ping, WANG Zhi-gang, DUAN Ting-yu. Effects of Claroideoglomus etunicatum and Grass Endophyte on the Growth of Lolium perenne under Different Phosphorus Levels [J]. Acta Agrestia Sinica, 2018, 26(6): 1458-1466.
[4]	SHI Chong, LU Yi, HUANG Chang-fu, ZHANG Rui-si, AN Sha-zhou. Using 70℃ Dry Treatment to Make Endophyte-free Seeds of Melica transsilvanica [J]. Acta Agrestia Sinica, 2016, 24(6): 1318-1322.
[5]	WAN Zhi-wen, WANG Ping, ZHANG Xing-xu, LI Chun-jie. Effects of Manganese and SA on Germination of Achnatherum inebrians Seed Containing or Free of Endophyte [J]. Acta Agrestia Sinica, 2016, 24(1): 107-113.
[6]	CAO Dan-dan, LU Hao, LI Xiao-meng, GUO Ya-zhou, XUE Rui-xu, WANG Huan, QUAN Hai-yun, ZHAO Bao-yu. Isolation, Identification and Diversity Analysis of Fungal Endophyte in Astragalus strictus [J]. Acta Agrestia Sinica, 2015, 23(6): 1252-1258.
[7]	ZHAO Xiao-jing, LI Xiu-zhang, Wang Ping, LI Chun-jie. Effects of Endophytic Fungi on the Seed Physiology of Hordeum brevisubulatum Under Artificial Ageing Treatment [J]. Acta Agrestia Sinica, 2015, 23(6): 1272-1277.
[8]	LIANG Jie, YANG Kai, LI Qin-fan, JIANG Zhen-guo, KONG Xiang-ya, YANG Guo-dong, JIN Yi-min. Isolation and Identification of Hemerocallin-Producing Fungal Endophyte [J]. , 2012, 20(5): 935-939.
[9]	GUO Wei, LI Qin-fan, KONG Xiang-ya, REN Jie, JING Xin-tang. Optimal C/N Ratio for Producing Swainsonine (SW) Using Locoweed Endophytes FEL3 [J]. , 2011, 19(4): 674-677.
[10]	SONG Mei-ling, LI Chun-jie, PENG Qing-qing, LIANG Ying, NAN Zhi-biao. Effects of Neotyphodium Endophyte on Germination of Hordeum brevisubulatum under Temperature and Water Stress Conditions [J]. , 2010, 18(6): 833-837.
[11]	ZHANG Xing-xu, LI Chun-jie, NAN Zhi-biao . Interactive Effect of Storage Time and Endophytic Fungi on Seed Physiology of Achnatherum inebrians(Hance) Keng [J]. , 2010, 18(2): 252-257.
[12]	WANG Zheng-feng, LI Chun-jie, JIN Wen-jin, NAN Zhi-biao. Effect of Neotyphodium Endophyte Infection on Salt Tolerance of Hordeum brevisubulatum(Trin.) Link [J]. , 2009, 17(1): 88-92.
[13]	ZHANG Xing-xu, CHEN Na, LI Chun-jie, NAN Zhi-biao . Physiological Effects of Rhopalosiphum padi on Achnatherum inebrians Keng Infected by Endophyte [J]. , 2008, 16(3): 239-244,261.