Influence of Simulation Nitrogen Deposition on Soil Microbial Functional Diversity of Calamagrostis angustifolia Wetland in Sanjiang Plain

doi:10.11733/j.issn.1007-0435.2016.06.011

Acta Agrestia Sinica ›› 2016, Vol. 24 ›› Issue (6): 1226-1233.DOI: 10.11733/j.issn.1007-0435.2016.06.011

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Influence of Simulation Nitrogen Deposition on Soil Microbial Functional Diversity of Calamagrostis angustifolia Wetland in Sanjiang Plain

SUI Xin^1,2, ZHANG Rong-tao¹, LIU Ying-nan¹, XU Nan¹, NI Hong-wei¹

1. Institute of Nature & Ecology, Heilongjiang Academy of Sciences, Harbin, Heilongjiang Province 150040, China;
2. Northeast Forestry University, Harbin, Heilongjiang Province 150040, China

Received:2015-12-22 Revised:2016-10-25 Online:2016-12-15 Published:2017-03-18

模拟氮沉降对三江平原小叶章湿地土壤微生物功能多样性的影响

隋心^1,2, 张荣涛¹, 刘赢男¹, 许楠¹, 倪红伟¹

1. 黑龙江省科学院自然与生态研究所, 黑龙江哈尔滨 150040;
2. 东北林业大学, 黑龙江哈尔滨 150040

通讯作者: 倪红伟
作者简介:隋心(1982-),男,黑龙江拜泉人,博士,副研究员,研究方向为生态学,E-mail:xinsui_cool@126.com
基金资助:
国家自然科学基金项目（31470019，31400429）；黑龙江省财政应用技术专项（STJB16-01，STJB2016-07）；黑龙江省科研机构创新能力提升专项计划（GY2014KC0039）；十三五国家重点研发计划（2016YFC0500405）资助

Abstract

Abstract:

To comprehensively understand the influence of nitrogen deposition on soil microbial carbon utilization characteristics of Calamagrostis angustifolia wetlands, the Biolog-Eco method was used. The results showed that there were significant differences in functional diversity of microbial communities at different nitrogen deposition treatments. AWCD increased with the extension of incubation time along with increasing SWC. The utilization intensity of six categories of carbon sources had differences and the amino acids were the most dominant carbon sources. Principal component analysis (PCA) identified that soil microbial carbon utilization at different nitrogen deposition treatments had obvious spatial differentiation, and horizontal zone difference of soil microbial functional diversity reflected in the use of carbohydrates, amino acids and amine, and the amine was particularly prominent. In addition, the correlation analysis of microbial diversity and soil physiochemical properties showed that total nitrogen, nitrate nitrogen, total P had a significant impact on soil microbial composition and functional activity in calamagrostis angustifolia wetlands. These results indicated that the soil microbial functional diversity changed by nitrogen deposition.

Key words: Wetland, Soil microorganism, Functional diversity, Biolog

摘要：

采用Biolog-Eco方法分析了模拟氮沉降对三江平原小叶章（Calamagrostis angustifolia）湿地土壤微生物碳源利用的影响。结果表明：不同氮沉降处理间土壤微生物功能多样性差异显著，AWCD值随培养时间延长而增加。土壤微生物对6大类碳源利用强度存在差异，各处理间土壤微生物对氨基酸类碳源利用率最高，为优势碳源；不同处理间土壤微生物在碳源利用上有明显的空间分异，土壤微生物功能多样性的差异主要体现在对羧酸类、酚酸类和胺类碳源的利用上，其中胺类尤为突出；此外，对不同施氮处理土壤微生物群落功能多样性与土壤理化因子相关分析表明，全氮、铵态氮、全磷会对小叶章湿地土壤微生物组成和功能活性产生显著影响。以上结果表明增加氮沉降改变了土壤微生物功能多样性。

关键词: 小叶章湿地, 土壤微生物, 功能多样性, Biolog

CLC Number:

Q938

SUI Xin, ZHANG Rong-tao, LIU Ying-nan, XU Nan, NI Hong-wei. Influence of Simulation Nitrogen Deposition on Soil Microbial Functional Diversity of Calamagrostis angustifolia Wetland in Sanjiang Plain[J]. Acta Agrestia Sinica, 2016, 24(6): 1226-1233.

隋心, 张荣涛, 刘赢男, 许楠, 倪红伟. 模拟氮沉降对三江平原小叶章湿地土壤微生物功能多样性的影响[J]. 草地学报, 2016, 24(6): 1226-1233.

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Influence of Simulation Nitrogen Deposition on Soil Microbial Functional Diversity of Calamagrostis angustifolia Wetland in Sanjiang Plain

模拟氮沉降对三江平原小叶章湿地土壤微生物功能多样性的影响

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