Cloning and Expression Analysis of MsSOS1 gene from Medicago sativa

doi:10.11733/j.issn.1007-0435.2018.06.028

Acta Agrestia Sinica ›› 2018, Vol. 26 ›› Issue (6): 1479-1489.DOI: 10.11733/j.issn.1007-0435.2018.06.028

Cloning and Expression Analysis of MsSOS1 gene from Medicago sativa

FANG Zhi-hong¹, Cui Miao-miao², ZHANG Yan¹, REN Bin-lin¹, SHI Yong-hong¹, LIU Jian-ning¹, WANG Yun-qi¹, DONG Kuan-hu³, GAO Hong-wen², WU Xin-ming¹

1. Animal Husbandry and Veterinary Institute, Shanxi Academy of Agricultural Sciences, Taiyuan, Shanxi Province 030032, China;
2. Institute of Aaimal Science, Chinese Academy of Agricultures Sciences, Beijing 100193, China;
3. College of Animal Science and Technology, Shanxi Agricultural University, Taigu, Shanxi Province 030801, China

Received:2018-08-06 Revised:2018-11-23 Online:2018-12-15 Published:2019-01-28

紫花苜蓿MsSOS1基因的克隆与表达分析

方志红¹, 崔苗苗², 张燕¹, 任彬琳¹, 石永红¹, 刘建宁¹, 王运琦¹, 董宽虎³, 高洪文², 吴欣明¹

1. 山西省农业科学院畜牧兽医研究所, 山西太原 030032;
2. 中国农业科学院北京畜牧兽医研究所, 北京 100193;
3. 山西农业大学动物科技学院, 山西太谷 030801

通讯作者: 吴欣明
作者简介:方志红(1983-),女,内蒙古赤峰人,博士,助理研究员,E-mail:fangzhihong2007@126.com
基金资助:
国家国际科技合作（R）专项；山西省农业科学院育种工程（17yzgc113）；中国农科院科技创新工程（ASTIP-IAS10）；现代农业产业技术体系专项资金（CARS-35-25）；山西省农业科学院所长青年引导专项项目（yydzx04）资助

Abstract

Abstract: Salt overly sensitive 1 (SOS1) gene is one of the essential genes in the biological process of plant against salt stress. In this study,a MsSOS1 gene was cloned from Medicago sativa leaves. It encodes 859 amino acids,has one conserved CAP-ED superfamily,one conserved Crp superfamily and one conserved Na⁺/H⁺ Exchanger superfamily. MsSOS1 has identity of 91%,87%,85%,84% and 77% of SOS1 gene from Cicer arietinum,Glycine max,Lupinus angustifolius,Arachis ipaensis and Vitis vinifera, respectively. Real-time quantitative PCR analysis revealed that MsSOS1 was expressed in root,stem,leaf and flower of Medicago sativa,with the highest expression in root and the lowest in flower. Besides,the expression of MsSOS1 gene could be regulated by the treatments of 4℃,PEG and ABA. This paper suggested that MsSOS1 involves in Medicago sativa stress tolerance.

Key words: Medicago sativa, MsSOS1, Adversity stress, Expression analysis

摘要： Na⁺/H⁺逆向转运蛋白基因SOS1（salt overly sensitive 1）是植物在抵御盐胁迫过程中一个重要的必需基因之一。本研究在紫花苜蓿（Medicago sativa）叶片中克隆得到一个MsSOS1基因，编码859个氨基酸，具有一个CAP-ED superfamily结构域、一个Crp superfamily结构域和一个Na⁺/H⁺ Exchanger superfamily结构域。与鹰嘴豆、大豆、羽扇豆、花生和葡萄的一致性分别是91%，87%，85%，84%和77%。实时荧光定量PCR分析表明，MsSOS1基因在根、茎、叶和花中均有表达，其中在根中的表达量最高，花中最低。此外，MsSOS1基因在4℃、PEG和ABA的胁迫中的表达均受到调控，推测该基因的表达与紫花苜蓿的抗逆性有关。

关键词: 紫花苜蓿, MsSOS1, 逆境胁迫, 表达分析

CLC Number:

S963.22+3.3

FANG Zhi-hong, Cui Miao-miao, ZHANG Yan, REN Bin-lin, SHI Yong-hong, LIU Jian-ning, WANG Yun-qi, DONG Kuan-hu, GAO Hong-wen, WU Xin-ming. Cloning and Expression Analysis of MsSOS1 gene from Medicago sativa[J]. Acta Agrestia Sinica, 2018, 26(6): 1479-1489.

方志红, 崔苗苗, 张燕, 任彬琳, 石永红, 刘建宁, 王运琦, 董宽虎, 高洪文, 吴欣明. 紫花苜蓿MsSOS1基因的克隆与表达分析[J]. 草地学报, 2018, 26(6): 1479-1489.

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Cloning and Expression Analysis of MsSOS1 gene from Medicago sativa

紫花苜蓿MsSOS1基因的克隆与表达分析

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