Cloning and Expression Analysis of AcABCG1 in Axonopus compressus

doi:10.11733/j.issn.1007-0435.2019.05.005

Acta Agrestia Sinica ›› 2019, Vol. 27 ›› Issue (5): 1147-1153.DOI: 10.11733/j.issn.1007-0435.2019.05.005

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Cloning and Expression Analysis of AcABCG1 in Axonopus compressus

LI Ji-fu¹, HAN Jia-rui¹, JIA Yi-dan¹, ZHANG Lang-zhi¹, WANG Wen-qiang², WANG Zhi-yong¹, CHEN Zhi-jian^1,2

1. Key Laboratory of Genetics and Germplasm Innovation of Tropical Special Forest Trees and Ornamental Plants, Ministry of Education, College of Forestry, Hainan University, Haikou, Hainan Province 570228, China;
2. Institute of Tropical Crop Genetic Resources, Chinese Academy of Tropical Agriculture Sciences/Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China, Ministry of Agriculture and Affairs, Haikou, Hainan Province 571101, China

Received:2019-08-16 Revised:2019-09-01 Online:2019-10-15 Published:2019-11-09

地毯草铝响应基因AcABCG1的克隆与表达分析

李季肤¹, 韩佳芮¹, 贾怡丹¹, 张郎织¹, 王文强², 王志勇¹, 陈志坚^1,2

1. 热带特色林木花卉遗传与种质创新教育部重点实验室/海南大学林学院, 海南海口 570228;
2. 中国热带农业科学院热带作物品种资源研究所/农业农村部华南作物基因资源与种质创制重点实验室, 海南海口 571101

通讯作者: 陈志坚, 王志勇
作者简介:李季肤(1995-),女,海南定安人,硕士研究生,主要从事草坪草抗逆性研究,E-mail:li_jifu@163.com
基金资助:
科技基础资源调查专项（2017FY100601）；中国热带农业科学院热带饲料作物与特色畜牧创新团队（1630032017031）；滇桂黔石漠化地区特色作物产业发展关键技术集成示范项目资助

Abstract

Abstract: Aluminum (Al) toxicity inhibits root growth and thus is considered as one of the important factors limiting crop yield in acid soils. ABC transporters participate in metal ion translocation and abiotic stress responses. In this study,AcABCG1,encoding an ABC transporter,was cloned from carpetgrass (Axonopus compressus) using PCR. Subsequently,the bioinformatic and gene expression analyses of AcABCG1 were performed. Results showed that the full length of AcABCG1 was 2 434 bp which encodes 811 amino acid residues. The molecular weight of AcABCG1 protein was 91.85 kD. Subcellular localization prediction showed that AcABCG1 localized to the plasma membrane. Quantitative PCR analysis showed that the expressions of AcABCG1 were enhanced in roots of carpetgrass being exposed to Al,Cd or La treatments. The transcriptional level of AcABCG1 was found to be regulated by Al treatments of different concentrations and duration. Furthermore,the transcript of AcABCG1 in roots was higher than that in stems and leaves. AcABCG1 was mainly expressed in the root tip with 0 to 1 cm in length. Taken together,this study provides the foundation for further study on the biological function of AcABCG1 gene in carpetgrass adaption to Al toxicity.

Key words: Carpetgrass, Al toxicity, ABC transporter, Gene cloning, Expression analysis

摘要： 铝毒抑制植物根系生长，是酸性土壤上限制作物产量的重要因素之一。ABC转运蛋白在金属离子转运和非生物胁迫应答等方面起作用。本研究以地毯草（Axonopus compressus）为材料，克隆了地毯草编码ABC转运蛋白AcABCG1基因，并对其进行了生物信息学和基因表达模式分析。结果表明，AcABCG1基因cDNA全长为2 434 bp，编码811个氨基酸残基，蛋白分子量为91.85 kD。亚细胞定位预测表明AcABCG1定位于细胞质膜上。定量PCR结果发现，金属铝（Al）、镉（Cd）和镧（La）处理均能显著增强AcABCG1基因在地毯草根系中的表达。不同铝浓度和时间处理进一步表明了AcABCG1基因在转录水平上响应铝胁迫。此外，AcABCG1基因在根中的表达量显著高于茎和叶中的表达量，且AcABCG1基因主要在0~1 cm根尖中表达。本研究结果为进一步挖掘AcABCG1基因参与地毯草耐铝毒的生物学功能奠定基础。

关键词: 地毯草, 铝毒, ABC转运蛋白, 基因克隆, 表达分析

CLC Number:

Q945.78

LI Ji-fu, HAN Jia-rui, JIA Yi-dan, ZHANG Lang-zhi, WANG Wen-qiang, WANG Zhi-yong, CHEN Zhi-jian. Cloning and Expression Analysis of AcABCG1 in Axonopus compressus[J]. Acta Agrestia Sinica, 2019, 27(5): 1147-1153.

李季肤, 韩佳芮, 贾怡丹, 张郎织, 王文强, 王志勇, 陈志坚. 地毯草铝响应基因AcABCG1的克隆与表达分析[J]. 草地学报, 2019, 27(5): 1147-1153.

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Cloning and Expression Analysis of AcABCG1 in Axonopus compressus

地毯草铝响应基因AcABCG1的克隆与表达分析

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