Acta Agrestia Sinica ›› 2021, Vol. 29 ›› Issue (1): 25-32.DOI: 10.11733/j.issn.1007-0435.2021.01.004

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Effects of the Alfalfa Post-harvest Rotation on Soil Bacterial Community in Arid Desert Oasis

NAN Li-li, GUO Quan-en, LIU Xue-qiang   

  1. College of Pratacultural Science, Gansu Agricultural University, Lanzhou, Gansu Province 730070, China
  • Received:2020-02-23 Revised:2020-05-27 Published:2021-01-08

荒漠灌区苜蓿后茬轮作不同作物对土壤细菌群落的影响

南丽丽, 郭全恩, 刘雪强   

  1. 甘肃农业大学草业学院, 甘肃 兰州 730070
  • 作者简介:南丽丽(1979-),女,甘肃天水人,博士,教授,主要从事草种质资源、栽培与育种研究,E-mail:nanll@gsau.edu.cn
  • 基金资助:
    国家自然科学基金(31460630);省部共建草业生态系统教育部重点实验室暨草学甘肃省优势特色学科建设(GAU-XKJS-2018-013);现代农业产业技术体系建设专项(CARS-34)共同资助

Abstract: In order to determine the effects of alfalfa post-harvest rotation on soil bacterial community in arid desert oasis,an experiment was set up with the following five crop rotation treatments:(Ⅰ)control (CK) with six-year-old alfalfa;(Ⅱ) 5-year-old alfalfa-1-year-old wheat (T1);(Ⅲ) 5-year-old alfalfa-1-year-old canola (T2);(Ⅳ) 5-year-old alfalfa-1-year-old sunflower (T3);and (Ⅴ) 5-year-old alfalfa-1-year-old corn (T4). The characteristics of soil bacteria in different treatments were analyzed by high-throughput sequencing (Illumina HiSeq). The results showed that T1and T2 treatments significantly increased soil microbial biomass carbon content. T4treatment significantly increased soil microbial biomass nitrogen content. T3 and T4 treatments significantly improved soil respiration. Soil urease,alkaline phosphatase,catalase,sucrase activities in T1,T2,T3,and T4 treatments were 1.44,1.13,1.36,and 1.54 times,1.53,7.78,4.25,and 7.28 times,1.09,1.28,1.14,and 1.06 times,1.11,1.38,1.14,and 1.74 times higher than that in CK treatment,respectively. Proteobacteria,Acidobacteria,Gemmatimonadetes,and Bacteroidetes were the four dominant bacteria in different treatments,accounting for 82.67% of all bacteria. Compared with CK,Proteobacteria,Gemmatimonadetes,and Actinobacteria decreased while Acidobacteria increased after different crop rotations. The abundance index (ACE and Chao1) of T1 and T2 treatments were significantly higher than CK (P<0.05). In conclusion,different crop rotations increased soil enzyme activities,stabilized bacterial community structure and improved soil productivity.

Key words: Alfalfa, Rotation, Bacterial community structure, High throughtput sequencing, Arid desert oasis

摘要: 为阐明荒漠灌区苜蓿(Medicago sativa)后茬轮作不同作物对土壤细菌群落的影响,本研究以6年生苜蓿地为对照(CK),以5年生苜蓿地翻耕灭茬分别种植1年小麦(Triticum aestivum)(T1)、油菜(Brassica chinensis)(T2)、葵花(Helianthus annuus)(T3)、玉米(Zea mays)(T4)为处理,采用高通量测序技术,分析不同处理下土壤细菌群落的变化特征。结果表明,T1,T2微生物量碳含量显著大于CK,T4微生物量氮含量显著高于CK(P<0.05);T3,T4土壤呼吸显著大于CK(P<0.05);T1,T2,T3,T4脲酶、碱性磷酸酶、过氧化氢酶、蔗糖酶活性分别是CK的1.44,1.13,1.36,1.54倍,1.53,7.78,4.25,7.28倍,1.09,1.28,1.14,1.06倍,1.11,1.38,1.14,1.74倍;与CK相比,轮茬后变形菌门、放线菌门、芽单胞菌门相对丰富呈降低、而酸杆菌门相对丰富呈增加趋势,这4个菌门为不同处理的优势菌门,占所有菌门的82.67%;T1,T2土壤细菌群落丰富度指数(Chao1和ACE)均显著高于CK(P<0.05)。综上,荒漠灌区轮茬较苜蓿连作更有助于提高土壤酶活性,稳定细菌群落结构,提高土地生产力。

关键词: 苜蓿, 轮茬, 细菌群落结构, 高通量测序, 荒漠灌区

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